Human tissue in vivo exists in 3D form, and culturing these in 2D format results in loss of function. Responses of 3D tissues to drugs, chemicals and cosmetics have been demonstrated to more closely predict human in vivo responses.
For more than 15 years, REPROCELL’s Chief Scientific Officer and Durham University Professor of Cell Technology Stefan Przyborski and his team have harnessed our award-winning Alvetex™ 3D scaffold technology to develop assays for testing pharmaceutical compounds in 3D models of human tissue e.g. skin, liver and neuronal aggregates.
|Above: StemRNA™ Neuro iPSC-derived human neurons.|
|Above: Recovery from neurite inhibitionTERA2.SP12 neurospheres transferred onto Alvetex Scaffold coated with four different ECM formulations. Data is from wild-type StemRNA AD Neuons (1.) positive control (laminin only); (2.) inhibitor control (laminin + aggrecan coating); (3.) ROCK rescue (laminin + aggrecan + Y-27632); (4.) Retinoid rescue (laminin + aggrecan + AC261066). Data show mean number of neurites present on the bottom of the Alvetex membrane at the end of the culture, ±SEM, n=3 independent repeats.|
Above: Representative morphology of ReproHepato™ cells after 6 days culture.
Above: (A.) Alvetex Scaffold 96-well plate Product. (B.) Scanning electron micrograph shows the highly porous architecture of Alvetex.
Alcyomics® has developed a full-thickness autologous skin equivalent model from healthy primary human tissue which is representative of normal human skin.
Above: Representative histological staining of a Skimune®3D Skin equivalent, clearly showing dermal compartment and differentiated epidermis with stratum corneum.
By combining the human relevance of REPROCELL’s iPSC-derived cell lines with our award-winning Alvetex™ 3D scaffold technology, our team at the REPROCELL Centre for Predictive Drug Discovery will work with you to develop a solution for your 3D in vitro assays needs. Whether you are an academic or industrial customer, for either contract research or in-house testing.
Predictive Drug Discovery Assay Services
Zara Puckrin, BSc / 17 March 2021
Zara Puckrin, BSc / 9 March 2021
Katerina Boididou, MSc / 3 February 2021
22 March 2021
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