Uterine Relaxation

Uterine Relaxation Assay: Ensuring Comprehensive Evaluation 

At REPROCELL, we offer the unique capability to evaluate both uterine contraction and uterine relaxation in response to your test article. Understanding how compounds affect uterine relaxation is crucial for developing therapies targeting conditions such as preterm labor and dysmenorrhea, where excessive contractions must be controlled to ensure patient safety and comfort. 

Key Features of Our Uterine Relaxation Assay 

• Broad Range of Reference Compounds: To accurately assess relaxation, we employ reference compounds including isoprenaline, isradipine, and sodium nitroprusside (SNP). 

• Precise Analysis: Both IC50 and pA2 values can be calculated, providing detailed insights into the test article’s potency and efficacy. 

• Comprehensive Testing: We test the effects of your compound in human and rat uterine tissue, offering a thorough comparison and validation of results across species. 

• GLP Compliance: All studies are conducted in accordance with GLP standards, ensuring the highest levels of safety and regulatory compliance. 

Our uterine relaxation assays use fresh human uterine tissue to simulate the natural rhythmic contraction and relaxation cycles. This allows us to measure the effect of potential therapeutic compounds on relaxation, providing essential data to guide your drug development process. 

Assay Methodology 

For uterine relaxation studies, we suspend strips of fresh human uterine muscle in organ baths, where the natural spontaneous contractions and relaxations are maintained. Following this, we expose the tissues to your test article, alongside positive controls such as isoprenaline, isradipine, and SNP, to determine the degree of relaxation achieved. 

• Reference Compounds: Isoprenaline, isradipine, and sodium nitroprusside (SNP) are well-known for their relaxing effects on uterine tissue. These compounds serve as benchmarks for evaluating the efficacy of your test article in inducing uterine relaxation. 

• Data Collection: We provide comprehensive data on the effects of the test article, including contraction frequency, amplitude reduction, and overall relaxation percentage. This allows for a complete understanding of the test article’s impact on uterine function. 

Supporting Your Drug Discovery 

At REPROCELL, our uterine relaxation assays deliver crucial insights for your drug discovery and development efforts, especially in the context of treating uterine hyperactivity disorders. Whether you are developing treatments for preterm labor, menstrual cramps, or other conditions involving uterine smooth muscle, our assays provide the precise data you need to move forward with confidence. 

In conjunction with our uterine contractility assays, our uterine relaxation studies ensure a holistic view of how your compound affects uterine tissue, helping you navigate the complexities of drug efficacy and safety with expert support.