Stemgent Protocols
Thawing, Passaging, Culturing, and Cryopreservation of Human MSCs (PDF)- Detection of AP using the Alkaline Phosphatase Staining Kit II (PDF)
- RNA Transfection using the Stemfect RNA Transfection Kit (PDF)
- hES Cell Culture using Stemedia Nutristem XF/FF Culture Medium (PDF)
- Cryopreservation of human ES cells using CryoStem Freezing Medium (PDF)
- Using the hES Cell Cloning and Recovery Supplement to Enhance Thawing and Recovery, Single Cell Cloning, and Passaging of hES Cells (PDF)
- Immunofluorescence Staining of Cells for Flow Cytometry (PDF)
- General Protocol for Immunocytochemistry (PDF)
- General Protocol for ICC Staining of Live Cells (PDF)
- Stemgent® StemRNA™ 3rd Gen Reprogramming Kit for Reprogramming Adult and Neonatal Human Fibroblasts (PDF)
- Protocol: Stemgent StemRNA 3rd Gen Reprogramming Kit for Reprogramming Urine-Derived Progenitor Cells (UPCs) (PDF)
- Protocol: Stemgent StemRNA 3rd Gen Reprogramming Kit for Reprogramming Urine-Derived Progenitor Cells (UPCs) (PDF)
- Stemgent® StemRNA™-NM Reprogramming Kit for Reprogramming Blood-Derived EPCs (PDF)
- Stemgent® StemRNA™-SR Reprogramming Using Blood-Derived EPCs (PDF)
- nippi iMatrix-511 Cell Culture Substrate use with Stemgent® StemRNA™-NM Reprogramming Kit and Stemgent NutriStem™ XF/FF Culture Medium (PDF)
- General Protocol for Western Blot Analysis (PDF)
Alvetex® Protocols
Alvetex Scaffold
1. Basics
- Alvetex Scaffold: quick start
- Media volumes guide for different feeding options in Alvetex Scaffold in well insert formats
- Alvetex Scaffold 96 well plate: quick start
- Alvetex Scaffold 96 well plate: instructions for use and example applications
- Alvetex Scaffold 384 well plate: quick start
- Alvetex Scaffold 384 well plate: instructions for use and example application
2. Coating
- Poly-D or L-lysine coating of Alvetex Scaffold
- Collagen I coating of Alvetex Scaffold
- Collagen I thin gel coating of Alvetex Scaffold
- Matrigel® coating of Alvetex Scaffold
- Fibronectin coating of Alvetex Scaffold
3. Cell-Specific
- Guidance notes and protocols for the culture of primary rat hepatocytes in Alvetex Scaffold in well insert and well plate formats
- Protocol for the culture of primary rat hepatocytes in Alvetex Scaffold 96 well plate format
- Culture of the canine kidney epithelial MDCK cell line on Alvetex Scaffold in well insert and well plate formats
- Example protocol for the culture of the MCF-10A cell line on Alvetex Scaffold (12 well insert format and 96-well plate format)
- Example protocol for the culture of the CaCo2 cell line on Alvetex Scaffold in well insert and well plate formats with or without a collagen I gel layer
- Example protocol for the co-culture of the Caco-2 cell line with the CCD-18co cell line on Alvetex Scaffold in well insert formats
- Example protocol for the culture of the breast carcinoma MDA-MB-231 cell line on Alvetex Scaffold in well insert and well plate formats
- Example protocol for the culture of the human colon fibroblastic CCD-18co cell line on Alvetex Scaffold in well insert and well plate formats
- Example protocol for the culture of the lung carcinoma A549 cell line on Alvetex Scaffold in well insert and well plate formats
- Example protocol for the culture of the breast invasive ductal carcinoma BT-474 cell line on Alvetex Scaffold
- Example protocol for the culture of the PC-3 cell line on Alvetex Scaffold in well plate and well insert formats
- Example protocol for the culture of the LN-229 cell line on Alvetex Scaffold in 96 well plate format
- Example protocol for the culture of the 3T3 cell line on Alvetex Scaffold in well plate and well insert formats
- Example protocol for the culture of the TERA2.cl.SP12 cell line on Alvetex Scaffold
- Example protocol for the culture of the HepG2 cell line on Alvetex Scaffold in well plate and well insert formats
- Example protocols for the culture of the CHO-K1 cell line on Alvetex Scaffold in well plate and well insert formats
- Example preparation of HaCaT epidermal equivalent using Alvetex Scaffold
- Bioengineering of human full thickness skin for in vitro applications, screening and product development
- Example protocol for the culture of the SW480 cell line on Alvetex Scaffold in well insert and well plate formats
- Example protocol for the culture of the SW620 cell line on Alvetex Scaffold in well insert and well plate formats
- Example protocols for the co-culture of either the SW620 or the SW480 cell line with the 3T3 cell line on Alvetex Scaffold in well insert formats
- Example protocol for the culture of the MG63 cell line on Alvetex Scaffold in well insert formats
- Example protocol for the culture of primary rat MSCs on Alvetex Scaffold in well insert formats
- Example protocols for the culture of the MCF-7 cell line on Alvetex Scaffold in well plate and well insert formats
4. Transfection
5. Cell Visualization
- Simple visualisation of cells on Alvetex Scaffold using light microscopy (Neutral Red-Methyl Blue dye)
- Live cell imaging of 3D cultures grown on Alvetex Scaffold using confocal microscopy
- Wheat germ agglutinin staining of 3D cultures grown on Alvetex Scaffold and Alvetex Strata
6. Metabolic Assays
- alamarBlue® cell viability assay of upcyte® hepatocyte cultures grown on Alvetex Scaffold in 3D
- Determination of cell number and growth characteristics within Alvetex Scaffold using the PicoGreen® assay
- XTT cell viability assay for Alvetex Scaffold 96 well plate format
- MTS cell viability assay of upcyte® hepatocyte cultures grown on Alvetex Scaffold in 3D
- MTT cell viability assay of cultures grown on Alvetex Scaffold in 3D
7. Genomics and Proteomics
- Total protein extraction from cells cultured in Alvetex Scaffold in 3D
- Protein extraction from cells cultured in Alvetex Scaffold 96 well plate format
- Extraction of RNA from cells cultured in Alvetex Scaffold in 3D
- Extraction of RNA from Cells Cultured in Alvetex Scaffold in 96-Well Plate Format
8. Histology
- Histology (1): Choosing the Right Fixative to Preserve 3D Cell Cultures
- Histology (2): Processing Alvetex Scaffold 3D Cultures for PFA Fixation and Paraffin Wax Embedding for Immunofluorescence
- Histology (3): Processing Alvetex Scaffold 3D Cultures for Bouin’s Fixation and Paraffin Wax Embedding for Haematoxylin and Eosin Staining
- Histology (4): Processing Alvetex Scaffold 3D Cultures for Resin Embedding and Toluidine Blue Staining for Light Microscopy
- Histology (5): Processing Alvetex Scaffold 3D Cultures for Scanning Electron Microscopy (SEM)
9. Advanced Microscopy
(See also Histology Series Part 5, above.)
- Processing Alvetex Scaffold 3D cultures for cryosectioning
- Immunofluorescence confocal microscopy of 3D cultures grown on Alvetex Scaffold
10. Cell Retrieval
- Retrieval of cells from Alvetex Scaffold
- Protocol for the removal of Alvetex discs from 6 well or 12 well inserts by unclipping the well insert base
11. 3D Perfused Cell Culture
Alvetex Strata
REPROCELL Protocols
- Cell Cultivation Protocol for ReproHepato™ (Cat.# RCDH001N) (PDF)
- Calcium imaging of ReproNeuro™ (Cat.# RCESDN001, RCESDN002, RCDN002N, RCDN003P) (PDF)
- Calcium imaging of ReproNeuro™ cells (Cat.# RCDN002N, RCDN003P, RCDN001N) (PDF)
- Protocol of ReproNeuro™ (Cat.# RCDN002N, RCDN003P) (PDF)
- Protocol of ReproNeuro™ (Cat.# RCDN002N, RCDN003P, RCDN001N) (PDF)
- ReproCardio 2™ (Cat.# RCDC001N) (PDF)
- Cryopreservation with ReproCryo DMSO Free™ (Cat.# RCHEFM002) (PDF)
- Feeder Cells (MEF) (Cat.# RCHEFC003) (PDF)
- Feeder Cells (SL10) (Cat.# RCHEFC001) (PDF)
- Freezing Medium for human ES/iPS Cells (Cat.# RCHEFM001) (PDF)
- Primate ES Cell Medium (Cat.# RCHEMD001) (PDF)
- ReproFF2™ (Cat.# RCHEMD006) (PDF)
- Dissociation Solution for human ES/iPS Cells (Cat.# RCHETP002) (PDF)
- ReproMed™ iPSC Medium (Cat.# RCRM101) (PDF)
- Primary Hepatocyte culture with ReproHP Medium™ (Cat. No. RCRM201 (and RCRM201-S)) (PDF)
Other Brands
ABLE Biott
NeuCyte
SynFire® Users Guide: Human iPSC-Derived Induced Neurons and Co-Culture System (PDF)- SynFire® iN plating protocol and chemical long-term potentiation on MaxWell Biosystems Multi-Well High-Density Microelectrode Array (HD-MEA) [Protocol] (PDF)
- Protocol for MED64 Presto 24-well Sakura MEA plates [Protocol] (PDF)
