Protocols
Stemgent® protocols
Alvetex® protocols
Alvetex® Scaffold
1. Basics
3. Cell-Specific
4. Transfection
5. Cell Visualization
6. Metabolic Assays
7. Genomics and Proteomics
Total protein extraction from cells cultured in Alvetex® Scaffold in 3D
Protein extraction from cells cultured in Alvetex® Scaffold 96 well plate format
Extraction of RNA from cells cultured in Alvetex® Scaffold in 3D
Extraction of RNA from Cells Cultured in Alvetex® Scaffold in 96-Well Plate Format
8. Histology
Histology (1): Choosing the Right Fixative to Preserve 3D Cell Cultures
Histology (2): Processing Alvetex® Scaffold 3D Cultures for PFA Fixation and Paraffin Wax Embedding for Immunofluorescence
Histology (3): Processing Alvetex® Scaffold 3D Cultures for Bouin’s Fixation and Paraffin Wax Embedding for Haematoxylin and Eosin Staining
Histology (4): Processing Alvetex® Scaffold 3D Cultures for Resin Embedding and Toluidine Blue Staining for Light Microscopy
Histology (5): Processing Alvetex® Scaffold 3D Cultures for Scanning Electron Microscopy (SEM)
11. 3D Perfused Cell Culture
Example Protocol using Alvetex® Scaffold 6 Well Inserts for 3D Perfused Cell Culture (Alvetex Perfusion Plate)
Alvetex® Advanced Tissue Bioengineering System
Media Volumes Guide for Different Feeding Options in the Alvetex® Advanced Tissue Bioengineering System
REPROCELL® protocols
Cell Cultivation Protocol for ReproHepato™ (Cat.# RCDH001N)
Calcium imaging of ReproNeuro™ (Cat.# RCESDN001, RCESDN002, RCDN002N, RCDN003P)
Calcium imaging of ReproNeuro™ cells (Cat.# RCDN002N, RCDN003P, RCDN001N)
Protocol of ReproNeuro™ (Cat.# RCDN002N, RCDN003P)
Protocol of ReproNeuro™ (Cat.# RCDN002N, RCDN003P, RCDN001N)
ReproCardio™ 2 (Cat.# RCDC001N)
Cryopreservation with ReproCryo™ DMSO Free (Cat.# RCHEFM002)
Feeder Cells (MEF) (Cat.# RCHEFC003)
Feeder Cells (SL10) (Cat.# RCHEFC001)
Freezing Medium for human ES/iPS Cells (Cat.# RCHEFM001)
Primate ES Cell Medium (Cat.# RCHEMD001)
ReproFF2™ (Cat.# RCHEMD006)
Dissociation Solution for human ES/iPS Cells (Cat.# RCHETP002)
ReproMed™ iPSC Medium (Cat.# RCRM101)
Primary Hepatocyte culture with ReproHP™ Medium (Cat. No. RCRM201 (and RCRM201-S))
Protocols from other brands
ABLE® Biott®
Operating instructions for ABLE® Biott® Bioreactor System