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Oligonucleotide Synthesis Services

India’s leading Oligosynthesis provider is now offering its services globally.

 

Bioserve India oligonucleotide boxes

Bioserve India (part of the REPROCELL Group) is the pioneer for oligonucleotide synthesis in India. Since 2002 Bioserve has been delivering the highest quality products. At our world-class facility, we synthesize large quantities of oligonucleotides using  high-throughput automated machines.
Oligonucleotide vial
We offer a complete line of custom oligonucleotide synthesis and purification services based on customer requirements. Our expertise in nucleic acid chemistry enables us to provide our clients with an extensive selection of oligo dyes, quenchers, modified bases, linkers, spacers, and other related conjugates.

Exceptional Quality Oligonucleotides, Delivered Worldwide

We test and approve ancillary reagents before use in our oligosynthesis production to ensure that they meet our high quality standards.

Our synthesis process is optimized and automated to meet stringent quality requirements.

We work according to GMP procedures. Each of our oligos comes with full traceability by extensive batch production records.

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Long Mer® DNA Oligos

We generate Long Mer DNA Oligonucleotides using proprietary synthesis methods, delivering high-quality Oligos up to 150 bases. These are available singly or as double-stranded and can be provided in tubes or plates.

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Modified DNA Oligos

We offer a wide variety of single and multiple modifications for DNA Oligonucleotides.

  • Fluorescent Labelled
  • Non-Fluorescent Labelled
  • Standard Modifications
  • q-PCR Probes

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NGS Grade® DNA Oligos

Our NGS Grade Oligos, for use in next-generation sequencing, are manufactured by proprietary methods that are proven to reduce index cross-talk and increase the success of multiplex NGS experiments.

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Universal Primer

We also manufacture complementary nucleotide sequences for amplifying very similar genes found in species of the same genus. Universal primers are designed to bind to a wide variety of DNA templates.

Oligo Quantity, Purity, and Modifications

  • We synthesize our oligonucleotides in 10, 20, 25, 50, 100, 200 and 1000 nanomole scales. (Other quantities available on request.)
  • We offer wide range of purification techniques – you choose what you want, based on your needs.
  • We offer an extensive selection of dyes, quenchers, modified bases, linkers, spacers, and other related conjugates.
  • We provide all our oligos desalted and lyophilized, ideal for shipping.
  • We follow stringent synthesis and QC parameters to provide exceptional quality products to our customers.

Oligo Quantities and Purification Techniques

Type of purification Length 5-35 5-35 5-50 5-100 5-120 5-150
Scale (nmole) 10 20 50 100 200 1000
Desalted OD @ 260 nm 5-7 7-8 8-10 15-20 21-25 30-35
OPC OD @ 260 nm 1-3 2-4 2-5 5-8 8-12 12-15
HPLC OD @ 260 nm — — 1-4 2-5 8-10 10-12
PAGE OD @ 260 nm — — 1-3 3-5 5-7 7-10

Standard Oligo Modifications

Modification Labeling position
5ʹ dd 3ʹ
Biotin ✓ 5-7 ✓
inosine ✓ ✓ —
Amino ✓ — ✓
Phosphorylation ✓ — ✓
Thiol ✓ — ✓

Quenchers

5ʹ Label 3ʹ Label
FAM BHQ-1
TET BHQ-1
HEX BHQ-1
TAMRA BHQ-1, BHQ-2
CY5 BHQ-2
CY3 BHQ-1

Fluorescent Dyes

  Labeling Position Purification Option
Fluorescent Dye Abs Em Ext. Coeff. MW 5ʹ Int 3ʹ HPSF HPLC PAGE Alternative to
TET 521 536 73 612.30 ✓ — — — ✓ — CAL Fluor Gold 540
HEX 536 556 73 681.20 ✓ — — — ✓ — CAL Fluor 560 SIMA
CY3 552 570 150 444.60 ✓ — — — ✓ — TYE 563
CY5 649 670 250 470.63 ✓ — — — ✓ — TYE 655
TAMRA 544 576 90 512.58
612.70		 ✓ — ✓ — ✓ — —
FAM 495 520 83 474.50 ✓ — ✓ — ✓ — ATTO 495

Ordering our Oligos is Easy

You can get in contact with us using the form below, and download our Oligo Order Form.

Download oligo order form

(Excel spreadsheet, 2.7 MB)

Fill out the spreadsheet form with your requirements, and return it to us when you place your order. We will send you a conformation, and let you know how soon we expect to complete your order.

When we have made your oligos and performed all the steps required, we will send you an Activity Report by email, and prepare to ship your Oligos to you, anywhere in the world.

Included with your oligos, we will send you the Activity Report. 

What’s in the Activity Report?

  • OD Units
  • Oligo concentrations in picomoles, nanomoles and micrograms
  • Melting Temperature (Tm) of the Oligo
  • Total Yield
  • GC Percentage
  • Molecular Weight
  • Reconstitution volume to get the 100μM concentration

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Ask our experts about our global oligosynthesis services