Oligonucleotide Synthesis Services

Bioserve India (part of the REPROCELL Group) is the pioneer for oligonucleotide synthesis in India. Since 2002 Bioserve has been delivering the highest quality products. At our world-class facility, we synthesize large quantities of oligonucleotides using high-throughput automated machines.

We offer a complete line of custom oligonucleotide synthesis and purification services based on customer requirements. Our expertise in nucleic acid chemistry enables us to provide our clients with an extensive selection of oligo dyes, quenchers, modified bases, linkers, spacers, and other related conjugates.

Exceptional Quality Oligonucleotides, Delivered Worldwide

We test and approve ancillary reagents before use in our oligosynthesis production to ensure that they meet our high quality standards.

Our synthesis process is optimized and automated to meet stringent quality requirements.

We work according to GMP procedures. Each of our oligos comes with full traceability by extensive batch production records.

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Long Mer® DNA Oligos

We generate Long Mer DNA Oligonucleotides using proprietary synthesis methods, delivering high-quality Oligos up to 150 bases. These are available singly or as double-stranded and can be provided in tubes or plates.

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Modified DNA Oligos

We offer a wide variety of single and multiple modifications for DNA Oligonucleotides.

Fluorescent Labelled
Non-Fluorescent Labelled
Standard Modifications
q-PCR Probes

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NGS Grade® DNA Oligos

Our NGS Grade Oligos, for use in next-generation sequencing, are manufactured by proprietary methods that are proven to reduce index cross-talk and increase the success of multiplex NGS experiments.

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Universal Primer

We also manufacture complementary nucleotide sequences for amplifying very similar genes found in species of the same genus. Universal primers are designed to bind to a wide variety of DNA templates.

Oligo Quantity, Purity, and Modifications

We synthesize our oligonucleotides in 10, 20, 25, 50, 100, 200 and 1000 nanomole scales. (Other quantities available on request.)
We offer wide range of purification techniques – you choose what you want, based on your needs.
We offer an extensive selection of dyes, quenchers, modified bases, linkers, spacers, and other related conjugates.
We provide all our oligos desalted and lyophilized, ideal for shipping.
We follow stringent synthesis and QC parameters to provide exceptional quality products to our customers.

Oligo Quantities and Purification Techniques

Type of purification	Length	5-35	5-35	5-50	5-100	5-120	5-150
Type of purification	Scale (nmole)	10	20	50	100	200	1000
Desalted	OD @ 260 nm	5-7	7-8	8-10	15-20	21-25	30-35
OPC	OD @ 260 nm	1-3	2-4	2-5	5-8	8-12	12-15
HPLC	OD @ 260 nm	—	—	1-4	2-5	8-10	10-12
PAGE	OD @ 260 nm	—	—	1-3	3-5	5-7	7-10

Standard Oligo Modifications

Modification	Labeling position
Modification	5ʹ	dd	3ʹ
Biotin	✓	5-7	✓
inosine	✓	✓	—
Amino	✓	—	✓
Phosphorylation	✓	—	✓
Thiol	✓	—	✓

Quenchers

5ʹ Label	3ʹ Label
FAM	BHQ-1
TET	BHQ-1
HEX	BHQ-1
TAMRA	BHQ-1, BHQ-2
CY5	BHQ-2
CY3	BHQ-1

Fluorescent Dyes

					Labeling Position			Purification Option
Fluorescent Dye	Abs	Em	Ext. Coeff.	MW	5ʹ	Int	3ʹ	HPSF	HPLC	PAGE	Alternative to
TET	521	536	73	612.30	✓	—	—	—	✓	—	CAL Fluor Gold 540
HEX	536	556	73	681.20	✓	—	—	—	✓	—	CAL Fluor 560 SIMA
CY3	552	570	150	444.60	✓	—	—	—	✓	—	TYE 563
CY5	649	670	250	470.63	✓	—	—	—	✓	—	TYE 655
TAMRA	544	576	90	512.58 612.70	✓	—	✓	—	✓	—	—
FAM	495	520	83	474.50	✓	—	✓	—	✓	—	ATTO 495

You can get in contact with us using the form below, and download our Oligo Order Form.

Download oligo order form

(Excel spreadsheet, 2.7 MB)

Fill out the spreadsheet form with your requirements, and return it to us when you place your order. We will send you a conformation, and let you know how soon we expect to complete your order.

When we have made your oligos and performed all the steps required, we will send you an Activity Report by email, and prepare to ship your Oligos to you, anywhere in the world.

Included with your oligos, we will send you the Activity Report.