REPROCELL Brand: Alvetex

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13-16 June: BIO International Convention 2022

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15-17 June: ISSCR Annual Meeting 2022

Cells grown in Alvetex maintain their in vivo morphology, behaviour and responsiveness within an in vitro model system.

Alvetex enables cells to maintain their natural shape and to freely interact with neighbouring cells in 3D so that they function in a more physiologically relevant manner.

Presented as a 200 µm thick membrane, Alvetex has been adapted to fit a variety of conventional cell culture plasticware formats. Each product unit has been terminally sterilised by gamma irradiation and remains sterile until its blister pack is opened. Alvetex requires an ethanol wash prior to use to render it hydrophilic. Alvetex does not degrade during normal use.

Main image above: scanning electron microscope (SEM) image of Alvetex Scaffold.

Alvetex enhances the biological relevance of your cell culture research

Typical mammalian cells are around 10-25 µm in size and are rarely further than 0-50 µm from another cell or 100-200 µm from a source of nutrients via a blood capillary. By recreating this complex cellular organisation and environment exprienced by cells within their native tissues, Alvetex 3D cell culture enables more accurate investigation into the study of cell behaviour and function compared with conventional 2D model systems.

Cells grow and divide occupying the 3D space within Alvetex (or in many instances, on top of Alvetex Strata), maintaining their natural shape and forming complex interactions with one another in a manner that closely mimics normal growth in tissues. The cells may lay down extra-cellular matrix which often leads to the formation of “mini-slab” tissue-like structures. Alvetex is compatible with a broad range of standard molecular, cellular and histological techniques.

Discover the science of Alvetex →

Growth of mammalian cells in 3D has been practiced for many decades using various materials and approaches. The Alvetex membrane was originally pioneered in the laboratory of Professor Stefan Przyborski at Durham University (UK).

Stefan has over 30 years of research experience within the fields of cell and tissue biology with specialization in stem cell science, cell differentiation and the development of advanced technologies that enable the construction of human tissues in vitro.

Prof. Przyborski’s interdisciplinary research at the boundaries of physical science and biology led to the development of Alvetex in the early 2000’s. He founded the company Reinnervate Ltd in 2002 with the purpose of commercializing and further developing Alvetex products and services.

Alvetex was named among the winners of The Scientist magazine’s “Top 10 Life Science Innovations of 2010” and was voted one of the Top 100 Innovative Products of 2011 at the R&D 100 Awards. Several other awards have since followed. Alvetex is arguably the market leading physical scaffold product that supports robust and routine 3D cell culture.

In 2013, Alvetex was chosen by a research group at Massachusetts General Hospital (USA) for 3D culturing of murine osteocytes on the International Space Station (ISS) for the study of bone loss during space flight. These microgravity experiments took place onboard the ISS in 2015.

Recognizing the future of 3D cell culture applications and tissue engineered in vitro models for drug discovery and regenerative medicine, REPROCELL Inc. (Japan) acquired Reinnervate in 2014 and merged it with Biopta Ltd. (Glasgow, UK) to form REPROCELL Europe Ltd. in 2016.

Reinnervate (formerly), the REPROCELL Group, Professor Przyborski’s academic lab at Durham University, and many scientists around the world have used Alvetex with success in their research. This research has generated significant amounts of data which has been published extensively in peer-reviewed journals. This body of scientific literature continues to grow year on year, and it exemplifies the use of Alvetex in multiple applications with many cell and tissue types.

Stefan Przyborski has remained active in the organization and currently serves as Chief Scientific Officer of the REPROCELL Europe Ltd, in addition to his academic role as Professor of Cell Technology in the department of Biosciences at Durham University (UK). He runs an active research laboratory at Durham University consisting of post-doctoral and postgraduate researchers. His group is well funded and regularly publishes their work in peer reviewed journals.

In both his Durham University lab and in REPROCELL Europe, Prof. Przyborski continues to lead research and development of new applications for 3D cell culture technologies and the generation of novel human tissue models that can be used in academia and industry for basic research, drug screening and safety assessment.

Alvetex 3D cell culture technology continues to be developed, produced and distributed by REPROCELL worldwide. And with our own expertise and experience, REPROCELL also now offers worldwide 3D cell culture and bioengineered human tissue model services built around the Alvetex platform.