Features and capabilities |
Alvetex 3D Cell Culture System |
Alvetex Advanced Tissue Bioengineering System |
Alvetex® Strata |
Alvetex® Scaffold |
Alvetex® Scaffold |
Supports routine 3D cell culture |
✓ |
✓ |
✓ |
Cells maintain their natural shape and can interact with neighbouring cells |
✓ |
✓ |
✓ |
Fibroblasts produce endogenous ECM negating need for additional coatings |
✓ |
✓ |
✓ |
Compatible with a wide range of standard molecular, cellular and histological techniques used in cell biology and bioedical laboratories |
✓ |
✓ |
✓ |
Inserts compatible with existing commercial culture plates |
✓ |
✓ |
✓ |
Mammalian cells adhere and grow primarily on the surface of the scaffold |
✓ |
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Mammalian cells grow primarily within the scaffold and on its suface |
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✓ |
✓ |
Designed to enable bioengineering of tissue constructs (e.g. human skin) |
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✓ |
✓✓✓ |
Unique set of inserts, handles, plates allows users great flexibility in experimental design, customising setup bespoke for specific applications |
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✓✓✓ |
Live culture models can be readily transferred between different plasticware formats |
✓✓ |
✓✓ |
✓✓✓ |
Compartible with measurement instrumentation (e.g. TEER, pH) |
|
✓ |
✓✓ |
Clinical / cosmetic testing instruments can be used on in vitro skin models |
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✓ |
✓✓✓ |
Dimensions of baseplates consistent with existing commercial plasticware enabling compatibility with robotics platforms, imaging setups, readers, etc. |
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✓✓✓ |
Unique design enables 3D culture models to be easily released for subsequent analysis by unclipping the bespoke insert and releasing the Alvetex disc |
✓ |
✓ |
✓ |
Live unclipped cultures and be re-assembled post-analysis and culture continued |
✓ |
✓ |
✓ |
Control of media volumes above and below culture model |
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✓ |
✓✓✓ |
Range of single well and multi-welled plates allowing greater control over media volumes |
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✓✓✓ |
Novel single welled plates allow sharing of media and paracrine signals between models promoting crosstalk during tissue differentiation and function |
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✓✓✓ |
Ability to handle and change media of multiple models simultaneously |
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✓✓✓ |
Easily raise and lower the inserts while in culture, e.g. to air interface |
✓ |
✓ |
✓✓✓ |
Enhanced perimeter seal around the model enabling more advanced barrier permation assays and testing passage of substances between upper and lower compartments |
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✓ |
Surface of the tissue construct readily accessible enabling application of test articles, measurements, imaging, wounding to surface, occulsion, etc. |
✓ |
✓ |
✓✓✓ |
Ability to access surface of the tissue construct in isolation of the growth medium below, minimising chances of media contamination |
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✓✓✓ |
Single welled plates and deep multi-welled plate can be used for general cell culture applications alone |
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✓✓✓ |