Alvetex Publications: 3D Cell Culture

Engineering a Pigmented Skin Equivalent That Is Responsive to External Stimuli

Paola De Los Santos Gomez, Kirsty Goncalves, Victoria Maltman, Lucy Smith & Stefan Przyborski
Skin Tissue Engineering
11 April 2025
DOI: 10.1007/978-1-0716-4510-9_9

Engineering a Multilayered Skin Equivalent: The Importance of Endogenous Extracellular Matrix and Model Flexibility for Nuanced and Accurate Skin Studies

Lucy Smith, Victoria Maltman, Paola De Los Santos Gomez, Kirsty Goncalves & Stefan Przyborski
Skin Tissue Engineering
11 April 2025
DOI: 10.1007/978-1-0716-4510-9_12

New Insights in Microplastic Cellular Uptake Through a Cell-Based Organotypic Rainbow-Trout (Oncorhynchus mykiss) Intestinal Platform

Nicole Verdile, Nico Cattaneo, Federica Camin, Matteo Zarantoniello, Federico Conti, Gloriana Cardinaletti, Tiziana A. L. Brevini, Ike Olivotto, and Fulvio Gandolfi
Cells
3 January 2025
DOI: 10.3390/cells14010044

Gastroesophageal circulating tumor cell crosstalk with peripheral immune system guides CTC survival and proliferation

Tania Rossi, Martina Valgiusti, Maurizio Puccetti, Giacomo Miserocchi, Michele Zanoni, Davide Angeli, Chiara Arienti, Ilaria Pace, Cristian Bassi, Ivan Vannini, Mattia Melloni, Erika Bandini, Milena Urbini, Massimo Negrini, Massimiliano Bonafè, Manuela Ferracin & Giulia Gallerani
Cell Death & Disease
29 March 2025
DOI: 10.1038/s41419-025-07530-2

Can Treatment with Human Mesenchymal Stem Cells Rescue the Degenerative Disc Phenotype? An in Vitro Pilot Study of Induced Cytokine Expression

Jonathan Dalton MD, Rajkishen Narayanan MD, Robert J Oris BS, Teeto Ezeonu BA, Evan Bradley BS, Jose A. Canseco MD PhD, Alexander R. Vaccaro MD PhD MBA, John D. Koerner MD, Dessislava Markova PhD, Christopher Kepler MD MBA
The Spine Journal
26 March 2025
DOI: 10.1016/j.spinee.2025.03.026

RNAi-based ALOX15B silencing augments keratinocyte inflammation in vitro via EGFR/STAT1/JAK1 signalling

Megan A. Palmer, Rebecca Kirchhoff, Claudia Buerger, Yvonne Benatzy, Nils Helge Schebb & Bernhard Brüne
Cell Death & Disease
22 January 2025
DOI: 10.1038/s41419-025-07357-x

A Bioengineered Model of the Human Cornea for Preclinical Assessment of Human Ocular Exposure to Environmental Toxicants

Se-jeong Kim, Ning Guo, Zong Yao Tan, Xing Gao, Pouria Fattahi, Haijiao Liu, Jeehan Chang, Mousa Younesi, Selice Jung, Yewhan Chung, Minkyung Song, Michelle Jung, Dan Dongeun Huh
Advanced Healthcare Materials
21 January 2025
DOI: 10.1002/adhm.202402408

Effects of Exosomes from MSC-iPSC on Fibroblasts in vitro in the Presence of Platelet-rich Plasma [PDF]

Andreea (Tibeică) Iordan, C. I. Crețu, S. C. Tibeică, Norina Consuela Forna, M. Costuleanu
Med. Surg. J. – Rev. Med. Chir. Soc. Med. Nat., Iaşi
2024
DOI: 10.22551/MSJ.2024.04.17

Disruption of epithelial barrier integrity via altered GILZ/c-Rel/RACK1 signaling in inflammatory bowel disease (IBD)

Erica Buoso, Mirco Masi, Roberta Valeria Limosani, Francesca Fagiani, Chiara Oliviero, Giorgia Colombo, Luigi Cari, Marco Gentili, Eleonora Lusenti, Lucrezia Rosati, Federica Pisati, Alessandra Pasini, Marco Vincenzo Lenti, Antonio Di Sabatino, Claire Louise Mobbs, Stefan Przyborski, Simona Ronchetti, Cristina Travelli, Marco Racchi
Journal of Crohn's and Colitis
18 Dec 2024
DOI: 10.1093/ecco-jcc/jjae191

Assessing the impact of airborne particulate pollution on human skin utilizing a novel human skin equivalent containing MUTZ-3-derived Langerhans cells

Amy Simpson, Teresa DiColandrea, Stefan Przyborski
Bioengineering and Translational Medicine
13 Dec 2024
DOI: 10.1002/btm2.10738

Comparative analysis of the effects of different hypoxia mimetic agents on the secretome contents of conditioned medium obtained from mesenchymal stem/stromal cells cultured in 2 or 3-dimensional cell culture systems

Serbay Ozkan, Basak Isildar & Meral Koyuturk
Cytotechnology
7 Dec 2024
DOI: 10.1007/s10616-024-00659-6

Bioengineering the Human Intestinal Mucosa and the Importance of Stromal Support for Pharmacological Evaluation In Vitro

Matthew Freer, Jim Cooper, Kirsty Goncalves, and Stefan Przyborski
Cells
8 Nov 2024
DOI: 10.3390/cells13221859

P13.06. A evaluation of BBB permeable WEE1 and CHK1 inhibitors in combination with standard of care for the treatment of Glioblastoma

E L Scholefield, N Gomez-Roman
Neuro-Oncology
5 Oct 2024
DOI: 10.1093/neuonc/noae144.239

Ank-mediated pyrophosphate regulates shear stress-induced small extracellular vesicle production in 3D-cultured osteocytes

Su Jeong Lee, Deuk Kju Jung, Soomin Im, Changkook You, Jung-Eun Kim, Jong-Sup Bae, Mee-seon Kim, Kyungmoo Yea & Eui Kyun Park
Animal Cells and Systems
22 Oct 2024
DOI: 10.1080/19768354.2024.2409460

Investigation into the significant role of dermal-epidermal interactions in skin ageing utilising a bioengineered skin construct

Lydia Costello, Kirsty Goncalves, Paola De Los Santos Gomez, Ben Hulette, Teresa Dicolandrea, Michael J. Flagler, Robert Isfort, John Oblong, Charlie Bascom, Stefan Przyborski
Journal of Cellular Physiology
8 Oct 2024
DOI: 10.1002/jcp.31463

Role of mesenchymal stem cell conditioned medium on wound healing using a developed 3D skin model

Moyassar Al-Shaibani, Xiao-nong Wang, Asif Tulah, Rachel E Crossland, Anne M Dickinson, Penny E Lovat
Journal of Wound Care
20 Aug 2024
DOI: 10.12968/jowc.2021.0397

Modulation of the Nogo signaling pathway to overcome amyloid-β-mediated neurite inhibition in human pluripotent stem cell-derived neurites

Kirsty Goncalves, Stefan Przyborski
Neural Regeneration Research
29 July 2024
DOI: 10.4103/NRR.NRR-D-23-01628

Exploring the Surface: Sampling of Potential Skin Cancer Biomarkers Kynurenine and Tryptophan, Studied on 3D Melanocyte and Melanoma Models

Sylwia Hasterok, Skaidre Jankovskaja, Ruzica Miletic Dahlström, Zdenka Prgomet, Lars Ohlsson, Sebastian Björklund, and Anna Gustafsson
Biomolecules
9 July 2024
DOI: 10.3390/biom14070815

Development of human pancreatic cancer avatars as a model for dynamic immune landscape profiling and personalized therapy

Deniel Hughes, Alice Evans, Simei Go, Michael Eyres, Liuliu Pan, Somnath Mukherjee, Zahir Soonawalla, Frances Willenbrock, Eric O’Neill
Science Advances
5 July 2024
DOI: 10.1126/sciadv.adm9071

The β-arrestin1/endothelin axis bolsters ovarian fibroblast-dependent invadosome activity and cancer cell metastatic potential

Danila Del Rio, Ilenia Masi, Valentina Caprara, Flavia Ottavi, Gabriele Albertini Petroni, Erica Salvati, Daniela Trisciuoglio, Sara Maria Giannitelli, Anna Bagnato, Emanuele Mauri, Francesca Spadaro & Laura Rosanò
Cell Death and Disease
22 May 2024
DOI: 10.1038/s41419-024-06730-6

Cell Senescence-Independent Changes of Human Skin Fibroblasts with Age

Nicola Fullard, James Wordsworth, Ciaran Welsh, Victoria Maltman, Charlie Bascom, Ryan Tasseff, Robert Isfort, Lydia Costello, Rebekah-Louise Scanlan, Stefan Przyborski, and Daryl Shanley
Cells
9 April 2024
DOI: 10.3390/cells13080659

Development of a 3D Brain Model to Study Sex-Specific Neuroinflammation After Hemorrhagic Stroke

Rezwanul Islam, Hadi Hasan Choudhary, Hritik Mehta, Feng Zhang, Tudor G. Jovin & Khalid A. Hanafy
Translational Stroke Research
16 March 2024
DOI: 10.1007/s12975-024-01243-y

Comparative analysis of effects of conditioned mediums obtained from 2D or 3D cultured mesenchymal stem cells on kidney functions of diabetic rats: Early intervention could potentiate transdifferentiation of parietal epithelial cell into podocyte precursors

Serbay Ozkan, Basak Isildar, Hakan Sahin, Halil Ibrahim Saygi, Dildar Konukoglu, Meral Koyuturk
Life Sciences
11 March 2024
DOI: 10.1016/j.lfs.2024.122543

Development and Optimisation of Tumour Treating Fields (TTFields) Delivery within 3D Primary Glioma Stem Cell-like Models of Spatial Heterogeneity

Callum G. Jones, Aurelie Vanderlinden, Ola Rominiyi, and Spencer J. Collis
Cancers
16 February 2024
DOI: 10.3390/cancers16050863

An in vitro model to study immune activation, epithelial disruption and stromal remodelling in inflammatory bowel disease and fistulising Crohn’s disease

Claire L. Mobbs, Nicole J. Darling and Stefan Przyborski
Frontiers in Immunology
12 February 2024
DOI: 10.3389/fimmu.2024.1357690

BRD9 status is a major contributor for cysteine metabolic remodeling through MST and EAAT3 modulation in malignant melanoma

Ana Hipólito, Renato Xavier, Cheila Brito, Ana Tomás, Isabel Lemos, Luís C. Cabaço, Fernanda Silva, Abel Oliva, Duarte C. Barral, João B. Vicente, Luís G. Gonçalves, Marta Pojo, Jacinta Serpa
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease
7 Decmber 2023
DOI: 10.1016/j.bbadis.2023.166983

3D human foreskin model for testing topical formulations of sildenafil citrate

Greta Camilla Magnano, Marika Quadri, Elisabetta Palazzo, Roberta Lotti, Francesca Loschi, Stefano Dall'Acqua, Michela Abrami, Francesca Larese Filon, Alessandra Marconi, Dritan Hasa
International Journal of Pharmaceutics
21 November 2023
DOI: 10.1016/j.ijpharm.2023.123612

Multi-tissue proteogenomic analysis for mechanistic toxicology studies in non-model species

MS Lin, MS Varunjikar, KK Lie, L Søfteland, L Dellafiora, R Ørnsrud, M Sanden, MHG Berntssen, JLCM Dorne, V Bafna, JD Rasinger
Environment International
7 November 2023
DOI: 10.1016/j.envint.2023.108309

Development of an ex vivo porcine skin model for the preclinical evaluation of subcutaneously injected biomacromolecules

Si Gou, Maria Lapteva, Romain Brusini, Killian Flegeau, François Bourdon, Gürkan Kaya, Jimmy Faivre, Yogeshvar N. Kalia
International Journal of Pharmaceutics
30 October 2023
DOI: 10.1016/j.ijpharm.2023.123562

An extramedullary bone model to study metastatic dormancy reveals a piecemeal model for metastatic reactivation.

Ilaria Malanchi, Stefania Di Blasio, Tatiana Rizou, Laurie Gay, Nicolas Rabas, Probir Chakravarty, Thomas Snoeks, Adam Karoutas, Felipe Rodrigues, Victoria Bridgeman, Xuanxuan Fan, Dominique Bonnet, Ander Abarrategi
Research Square
27 July 2023
DOI: 10.21203/rs.3.rs-3173228/v1

Resolvin D1 improves airway inflammation and exercise capacity in cystic fibrosis lung disease

Giulia Ferri, Matteo Serano, Elisa Isopi, Matteo Mucci, Domenico Mattoscio, Romina Pecce, Feliciano Protasi, Marcus A. Mall, Mario Romano, Antonio Recchiuti
FASEB Journal
12 October 2023
DOI: 10.1096/fj.202301495R

Evaluation of rainbow trout (Oncorhynchus mykiss) organotypic intestinal platforms: cellular responses after long-term exposure to in vitro digested feed

Nicole Verdile, Federica Camin, Marcelo A. Chacon, Rolando Pasquariello, Radmila Pavlovic, David Peggs, Ramon Fontanillas, Amos Tandler, Trond M. Kortner, Amir Bitan, Tiziana A. Brevini and Fulvio Gandolfi
Front. Mar. Sci.
7 July 2023
DOI: 10.3389/fmars.2023.1239682

A novel study of melatonin diffusion in a 3-D cell culture model

Francisco Artime-Naveda, Lucas Alves-Pérez, David Hevia, Sergio Alcón-Rodríguez, Sheila Fernández-Vega, Alejandro Alvarez-Artime, Isabel Quirós-González, Rafael Cernuda, Rosa María Sainz, Juan Carlos Mayo
Melatonin Research
13 June 2023
DOI: 10.32794/mr112500148

The NF–Y splicing signature controls hybrid EMT and ECM-related pathways to promote aggressiveness of colon cancer

Giovanna Rigillo, Silvia Belluti, Virginia Campani, Gregorio Ragazzini, Mirko Ronzio, Giacomo Miserocchi, Beatrice Bighi, Laura Cuoghi, Valentina Mularoni, Vincenzo Zappavigna, Diletta Dolfini, Laura Mercatali, Andrea Alessandrini, Carol Imbriano
Cancer Letters
10 June 2023
DOI: 10.1016/j.canlet.2023.216262

Cruciferous vegetable-derived indole-3-carbinol prevents coronavirus cell egression mechanisms in tracheal and intestinal 3D in vitro models.

Georgia Pennarossa, Sharon Arcuri, Rolando Pasquariello, Fulvio Gandolfi, Margherita Maranesi, Tiziana A.L. Brevini
Phytochemistry
12 May 2023
DOI: 10.1016/j.phytochem.2023.113713

Generation of a novel three-dimensional scaffold-based model of the bovine endometrium

MC. Díez, S. Przyborski, A. del Cerro, M. Alonso-Guervós, T. Iglesias-Cabo, S. Carrocera, MA. García, M. Fernández, L. Alonso & M. Muñoz
Veterinary Research Communications
8 May 2023
DOI: 10.1007/s11259-023-10130-0

Exploitation of In Vivo-Emulated In Vitro System in Advanced Food Science

Yonglu Li, Ping Li, Xin Yu, Xiaodong Zheng, and Qing Gu
J Agric Food Chem
6 April 2023
DOI: 10.1021/acs.jafc.2c07289

Safety of Gold Nanoparticles: From In Vitro to In Vivo Testing Array Checklist

Joana Lopes, Tânia Ferreira-Gonçalves, Lia Ascensão, Ana S. Viana, Lina Carvalho, José Catarino, Pedro Faísca, Abel Oliva, Dragana P. C. de Barros, Cecília M. P. Rodrigues, Maria Manuela Gaspar and Catarina Pinto Reis
J Pharmaceutics
31 March 2023
DOI: 10.3390/pharmaceutics15041120

Type I interferon-dependent IFIT3 signaling is critical for viral clearance in airway neutrophils

Amit Gaggar, Camilla Margaroli, Timothy Fram, Nirmal Sharma, Siddharth Patel, Jennifer Tipper, Sarah Robison, Derek Russell, Seth Fortmann, Mudassir Banday, Tarek Abdalla, Sawanan Saitornuang, Matthew Madison, Sixto Leal, Kevin Harrod, Nathaniel Erdmann
Research Square
21 March 2023
DOI: 10.21203/rs.3.rs-1812836/v1 (pre-print)

Abstract: Neutrophilic inflammation characterizes several respiratory viral infections including COVID-19-related ARDS, although its contribution to disease pathogenesis remains poorly understood. Here, we identified two neutrophil subpopulations (A1 and A2) in the airway compartment of 52 severe COVID-19 subjects, where loss of the A2 subset correlated with increased viral burden and reduced 30-days survival. A2 neutrophils showcased a discrete antiviral response with an increased interferon signature. Blockade of type I interferon attenuated viral clearance in A2 neutrophils and downregulated IFIT3 and key catabolic genes, demonstrating direct antiviral neutrophil function. Knockdown of IFIT3 in A2 neutrophils led to loss of IRF3 phosphorylation with consequent reduced viral catabolism, providing the first discrete mechanism of type I interferon signaling in neutrophils. The identification of this novel neutrophil phenotype and its association with severe COVID-19 outcomes emphasizes its likely importance in other respiratory viral infections and potential for new therapeutic approaches in viral illness.

Regulation of epidermal proliferation and hair follicle cycling by synthetic photostable retinoid EC23

Arto Määttä, Rebecca Nixon, Neil Robinson, Carrie A Ambler, Kirsty Goncalves, Victoria Maltman, Stefan Przyborski
J Cosmet Dermatol
03 January 2023
DOI: 10.1111/jocd.15629

The interaction of β-arrestin1 with talin1 driven by endothelin A receptor as a feature of α5β1 integrin activation in high-grade serous ovarian cancer

Ilenia Masi, Flavia Ottavi, Danila Del Rio, Valentina Caprara, Cristina Vastarelli, Sara Maria Giannitelli, Giulia Fianco, Pamela Mozetic, Marianna Buttarelli, Gabriella Ferrandina, Giovanni Scambia, Daniela Gallo, Alberto Rainer, Anna Bagnato, Francesca Spadaro & Laura Rosanò
Cell Death & Disease
30 January 2023
DOI: 10.1038/s41419-023-05612-7

Biomaterial-based in vitro 3D modeling of glioblastoma multiforme

Tanvir Ahmed
Cancer Pathogenesis and Therapy
9 January 2023
DOI: 10.1016/j.cpt.2023.01.002

Development of a mammalian neurosensory full-thickness skin equivalent and its application to screen sensitizing stimuli

Freer M, Darling N, Goncalves K, Mills KJ, Przyborski S.
Bioeng Transl Med
January 2023
DOI: 10.1002/btm2.10484

2D and 3D cultured human umbilical cord-derived mesenchymal stem cell-conditioned medium has a dual effect in type 1 diabetes model in rats: immunomodulation and beta-cell regeneration

Basak Isildar, Serbay Ozkan, Merve Ercin, Selda Gezginci-Oktayoglu, Mahmut Oncul & Meral Koyuturk
Inflammation and Regeneration
30 November 2022
DOI: 10.1186/s41232-022-00241-7

Application of biomimetic surfaces and 3D culture technology to study the role of extracellular matrix interactions in neurite outgrowth and inhibition

K.E.Goncalves, S.Phillips, D.S.H.Shah, D.Athey, S.A.Przyborski
Biomaterials Advances
23 November 2022
DOI: 10.1016/j.bioadv.2022.213204

Investigation into the effect of skin tone modulators and exogenous stress on skin pigmentation utilizing a novel bioengineered skin equivalent

Kirsty Goncalves, Paola De Los Santos Gomez, Lydia Costello, Lucy Smith, Hugh Mead, Amy Simpson, Stefan Przyborski
Bioengineering and Translational Medicine
26 September 2022
DOI: 10.1002/btm2.10415

Early functional mismatch between breast cancer cells and their tumour microenvironment suppresses long term growth

Anna Perdrix Rosell, Oscar Maiques, Joshua Alexander James Martin, Probir Chakravarty, Luigi Ombrato, Victoria Sanz-Moreno, Ilaria Malanchi
Cancer Letters
28 September 2022
DOI: 10.1016/j.canlet.2022.215800

Macrophage PD-1 associates with neutrophilia and reduced bacterial killing in early cystic fibrosis airway disease

Camilla Margaroli, Hamed Horati, Luke W.Garratt, Vincent D. Giacalone, Craig Schofield, A. Susanne Dittrich, Tim Rosenow, Brian S.Dobosh, Hong S.Lim, Dario L.Frey, Mieke Veltman, George L.Silva, Milton R.Brown, Carsten Schultz, Harm A.W.M.Tiddens, Sarath Ranganathang, Joshua D.Chandler, Peng Qiu, Limin Peng, Bob J.Scholte, Marcus A.Mall, Anthony Kicic, Lokesh Guglani, Stephen M.Stick, Hettie M.Janssens, Rabindra Tirouvanziam
Journal of Cystic Fibrosis
19 June 2022
DOI: 10.1016/j.jcf.2022.06.001

Modelling liver cancer microenvironment using a novel 3D culture system

Ala’a Al Hrout, Karla Cervantes-Gracia, Richard Chahwan & Amr Amin
Scientific Reports 12, Article number: 8003 (2022)
14 May 2022
DOI: 10.1038/s41598-022-11641-7

Recreating Tissue Structures Representative of Teratomas In Vitro Using a Combination of 3D Cell Culture Technology and Human Embryonic Stem Cells

Alejandro Hidalgo Aguilar, Lucy Smith, Dominic Owens, Rebecca Quelch, and Stefan Przyborski
Bioengineering 2022, 9(5), 185
22 April 2022
DOI: 10.3390/bioengineering9050185

In vitro toxicity of glyphosate in Atlantic salmon evaluated with a 3D hepatocyte-kidney co-culture model

L.Søfteland and P.A.Olsvik
Food and Chemical Toxicology, Volumn 164
14 April 2022
DOI: 10.1016/j.fct.2022.113012

Biomimetic Full-Thickness Skin-on-a-Chip Based on a Fibroblast-Derived Matrix

Patrícia Zoio, Sara Lopes-Ventura, and Abel Oliva
Micro (MDPI)
12 March 2022
DOI: 10.3390/micro2010013

A Novel Coculture System for Assessing Respiratory Sensitizing Potential by IL-4 in T Cells (PDF)

Izuru Mizoguchi, Yasuhiro Katahira, Shinya Inoue, Eri Sakamoto, Aruma Watanabe, Yuma Furusaka, Atsushi Irie, Satoru Senju, Yasuharu Nishimura, Shusaku Mizukami, Kenji Hirayama, Sou Nakamura, Koji Eto, Hideaki Hasegawa and Takayuki Yoshimoto
ALTEX
24 February 2022
DOI: 10.14573/altex.2111181

Radiation exposure elicits a neutrophil-driven response in healthy lung tissue that enhances metastatic colonization

Emma Nolan, Victoria Louise Bridgeman, Luigi Ombrato, Adam Karoutas, Nicolas Rabas, Celine Angeli Natascha Sewneth, Marcos Vasquez, Felipe Silva Rodrigues, Stuart Horswell, Peter Faull, Rebecca Carter & Ilaria Malanchi
Nat Cancer 3, 173–187 (2022)
24 February 2022
DOI: 10.1038/s43018-022-00336-7

Neutrophil-derived extracellular vesicles promote feed-forward inflammasome signaling in cystic fibrosis airways

Osric A Forrest, Brian Dobosh, Sarah A Ingersoll, Sanjana Rao, Alexander Rojas, Julie Laval, Jessica A Alvarez, Milton R Brown, Vin Tangpricha, Rabindra Tirouvanziam
J Leuk Biol
16 February 2022
DOI: 10.1002/JLB.3AB0321-149R

Transcriptomic and Functional Evidence Show Similarities between Human Amniotic Epithelial StemCells and Keratinocytes

Li-Ping Liu, Dong-Xu Zheng, Zheng-Fang Xu, Hu-Cheng Zhou, Yun-Cong Wang, Hang Zhou, Jian-Yun Ge, Daisuke Sako, Mi Li, Kazunori Akimoto, Yu-Mei Li, and Yun-Wen Zheng
Cells 2022, 11(1), 70
27 December 2021
DOI: 10.3390/cells11010070

miR-378d suppresses malignant phenotype of ESCC cells through AKT signaling

Jie Peng, Susu Shi, Juan Yu, Jianli Liu, Haixiang Wei, Haixia Song, Shaoqiang Wang, Zhejie Li, Shujin He, Lei Li, Hongyan Zhang, Zhizhen Yan, Ran Zhao, Yukun Liu, Yanrong Liu, Junjun Li, Renya Zhang and Wei Wang
Cancer Cell International volume 21, Article number: 702 (2021)
22 December 2021
DOI: 10.1186/s12935-021-02403-y

Mass production of human airway-like neutrophils via transmigration in an organotypic model of human airways

Brian Dobosh, Vincent D Giacalone, Camilla Margaroli, Rabindra Tirouvanziam
Star Protocols. Volume 2, Issue 4
17 December 2021
DOI: 10.1016/j.xpro.2021.100892

Mechanistic analysis and significance of sphingomyelinasemediated decreases in transepithelial CFTR currents in nHBEs

Kirsten A Cottrill, Vincent D Giacalone, Camilla Margaroli, Robert J Bridges, Michael Koval, Rabindra Tirouvanziam, Nael A McCarty
Physiological Reports.
September 2021
DOI: 10.14814/phy2.15023

Derivation of Adult Human Cortical Organotypic Slice Cultures for Coculture with Reprogrammed Neuronal Cells

Giedre Kvist
Methods in Molecular Biology (MIMB), volume 2352
30 July 2021
DOI: 10.1007/978-1-0716-1601-7_17

Barrier-on-a-Chip with a Modular Architecture and Integrated Sensors for Real-Time Measurement of Biological Barrier Function

Patrícia Zoio, Sara Lopes-Ventura and Abel Oliva
Micromachines 2021, 12(7), 816
12 July 2021
DOI: 10.3390/mi12070816

Pigmented full-thickness human skin model based on a fibroblast-derived matrix for long-term studies

Mrs Patrícia Zoio, Mrs Sara Ventura, Mrs Mafalda Leite, and Dr Abel Oliva
Tissue Engineering Part C: Methods
19 June 2021
DOI: 10.1089/ten.TEC.2021.0069

miR-378d regulates polyploidization and malignant phenotype of tumor cells through AKT and RhoA

Jie Peng, Susu Shi, Juan Yu, Jianli Liu, Haixiang Wei, Haixia Song, Shaoqiang Wang, Daolu Guo, Zhejie Li, Shujin He, Lei Li, Hongyan Zhang, Zhizhen Yan, Ran Zhao, Yukun Liu, Yanrong Liu, Junjun Li, Renya Zhang, Wei Wang
Preprints 2021, 2021060443
16 June 2021
DOI: 10.20944/preprints202106.0443.v1

Immune modulatory effects of Idelalisib in stromal cells of chronic lymphocytic leukemia

Sarah Handl, Franziska von Heydebrand, Simon Voelkl, Robert A J Oostendorp, Jochen Wilke, Anita N Kremer, Andreas Mackensen, and Gloria Lutzny-Geier
Leukemia and Lymphoma
17 May 2021
DOI: 10.1080/10428194.2021.1927019

Using Advanced Cell Culture Techniques to Differentiate Pluripotent Stem Cells and Recreate Tissue Structures Representative of Teratoma Xenografts

L A Smith, A Hidalgo Aguilar, D D G Owens, R H Quelch, E Knight and S A Przyborski
Frontiers in Cell Development and Biology
06 May 2021
DOI: 10.3389/fcell.2021.667246

Extracellular Vesicles From a Three-Dimensional Culture of Perivascular Cells Accelerate Skin Wound Healing in a Rat

Min Ho Kim, Changho Chung, Mun Ho Oh, Jin Hyun Jun, Yong Ko and Jong Hun Lee
Aesthetic Plastic Surgery
05 April 2021
DOI: 10.1007/s00266-021-02254-y

SIRT1 Inhibits Apoptosis by Promoting Autophagic Flux in Human Nucleus Pulposus Cells in the Key Stage of Degeneration via ERK Signal Pathway

Fei He, Qingshu Li, Bo Sheng, Haitao Yang, and Wei Jiang
BioMed Research International, vol. 2021, Article ID 8818713.
DOI: 10.1155/2021/8818713

Use of Porous Polystyrene Scaffolds to Bioengineer Human Epithelial Tissues In Vitro

Costello L, Darling N, Freer M, Bradbury S, Mobbs C, Przyborski S
Methods in Molecular Biology (Clifton, N.J.)
1 January 2021
DOI: 10.1007/978-1-0716-1246-0_20

Generation of Keratinocytes from Human Induced Pluripotent Stem Cells Under Defined Culture Conditions

Shyam Kishor Sah, Jitendra K Kanaujiya, I-Ping Chen, and Ernst J Reichenberger
Cellular Reprogramming
29 December 2020
DOI: 10.1089/cell.2020.0046

The human bone marrow harbors a CD45⁻CD11B⁺ cell progenitor permitting rapid microglia-like cell derivative approaches

Andreas Bruzelius Isabel Hidalgo Antonio Boza-Serrano Anna-Giorgia Hjelmér Amelie Tison Tomas Deierborg Johan Bengzon Tania Ramos-Moreno
Stem Cells Translational Medicine
09 December 2020
DOI: 10.1002/sctm.20-0127

Exposure of the SH-SY5Y Human Neuroblastoma Cells to 50-Hz Magnetic Field: Comparison Between Two-Dimensional (2D) and Three-Dimensional (3D) In Vitro Cultures

Claudia Consales, Alessio Butera, Caterina Merla, Emanuela Pasquali, Vanni Lopresto, Rosanna Pinto, Maria Pierdomenico, Mariateresa Mancuso, Carmela Marino and Barbara Benassi
Molecular Neurobiology
24 November 2020
DOI: 10.1007/s12035-020-02192-x

Developing a novel 3D model of the intestinal epithelium as a tool to study the pharmacokinetics of new chemical entities

Matthew A Freer
Doctoral Thesis, Durham University, UK
25 September 2020

Bioengineering Novel in vitro Co-culture Models That Represent the Human Intestinal Mucosa With Improved Caco-2 Structure and Barrier Function

Nicole J Darling, Claire L Mobbs, Ariana L González-Hau, Matthew Freer and Stefan Przyborski
Frontiers in Bioengineering and Biotechnology
31 August 2020
DOI: 10.3389/fbioe.2020.00992

E7 oncoprotein from human papillomavirus 16 alters claudins expression and the sealing of epithelial tight junctions

Perla Yaceli Uc, Jael Miranda, Arturo Raya-Sandino, Lourdes Alarcón, María Luisa, Roldán Rodolfo, Ocadiz-Delgado, Enoc Mariano Cortés-Malagón, Bibiana Chávez-Munguía, Georgina Ramírez, René Asomoza, Liora Shoshani, Patricio Gariglio, Lorenza González-Mariscal
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Targeting of Beta Adrenergic Receptors Results in Therapeutic Efficacy against Models of Hemangioendothelioma and Angiosarcoma

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Public Library of Science. PLoS ONE 8(3): e60021
28 March 2013
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Characterization of liver specific functions of upcyte® hepatocytes grown in 3D and co-cultured with upcyte® endothelial cells

Dähn C, Hewitt N, Maltman D, Talas G, Przyborski S, Heinz S, Nörenberg A, Scheller K, Braspenning J
Z Gastroenterol, 2012; 50 - P2_05
2012
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Generation of proliferating human hepatocytes using upcyte® technology: characterisation and applications in induction and cytotoxicity assays

Alexandra Burkard, Caroline Dähn, Stefan Heinz, Anne Zutavern, Vera Sonntag-Buck, Daniel Maltman, Stefan Przyborski, Nicola J. Hewitt, and Joris Braspenning
Xenobiotica
October 2012
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The autophagy-associated factors DRAM1 and p62 regulate cell migration and invasion in glioblastoma stem cells

Galavotti S, Bartesaghi S, Faccenda D, Shaked-Rabi M, Sanzone S, McEvoy A, Dinsdale D, Condorelli F, Brandner S, Campanella M, Grose R, Jones C, Salomoni P
Oncogene
23 April 2012
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Rat primary hepatocytes show enhanced performance and sensitivity to acetaminophen during three-dimensional culture on a polystyrene scaffold designed for routine use

Schutte M, Fox B, Baradez M, Devonshire A, Minguez J, Bokhari M, Przyborski S, Marshall D
Assay and Drug Development Technologies
October 2011
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Dysregulated TRK signalling is a therapeutic target in CYLD defective tumours

Rajan N, Elliott R, Clewes O, Mackay A, Reis-Filho JS, Burn J, Langtry J, Sieber-Blum M, Lord CJ, Ashworth A
Oncogene
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Adipose tissue-derived stem cells display a proangiogenic phenotype on 3D scaffolds.

Neofytou EA, Chang E, Patlola B, Joubert LM, Rajadas J, Gambhir SS, Cheng Z, Robbins RC, Beygui RE
Journal Biomedical Materials Research Part A
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Alvetex: Polystyrene scaffold technology for routine three dimensional cell culture.

Knight E, Murray B, Carnachan R, Przyborski SA
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Validation of reference gene stability for APAP hepatotoxicity studies in different in vitro systems and identification of novel potential toxicity biomarkers

Bridget C Fox, Alison S Devonshire, Maaike E Schutte, Carole A Foy, Jesus Minguez, Stefan Przyborski, Daniel Maltman, Maria Bokhari, Damian Marshall
Toxicology In Vitro, Volume 24, Issue 7, October 2010, Pages 1962-1970
October 2010
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Developments in three dimensional cell culture technology aimed at improving the accuracy of in vitro analyses

Maltman D, Przyborski SA
Biochemical Society Transactions (2010), 38(4), 1072-5
August 2010
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Emulsion-templated porous polymers as scaffolds for three dimensional cell culture: effect of synthesis parameters on scaffold formation and homogeneity

Carnachan RJ, Bokhari M, Maatta A, Cameron NR, Przyborski SA

Journal or Materials Chemistry
12 Jul 2007
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Culture of HepG2 liver cells on three dimensional polystyrene scaffolds enhances cell structure and function during toxicological challenge

Bokhari M, Carnachan R, Cameron NR, Przyborski SA
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Effect of synthesis parameters on emulsion-templated porous polymer formation and evaluation for 3D cell culture scaffolds

Bokhari M, Carnachan R, Przyborski SA, Cameron NR
Journal of Materials Chemistry (2007), 17, 4088-4094
12 July 2007
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Novel cell culture device enabling three-dimensional cell growth and improved cell function

Bokhari M, Carnachan R, Cameron NR, Przyborski, SA
Biochemical and Biophysical Research Communications (2007), 354, 1095-1100
6 January 2007
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Tailoring the morphology of emulsion-templated porous polymers

Carnachan RJ, Bokhari M, Przyborski SA, Cameron NR
Soft Matter (2006), 2, 608-616
23 May 2006
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Porous polymers by emulsion templating

Barbetta A, Carnachan RJ, Smith KH, Zhao C, Cameron NR, Kataky R, Hayman M, Przyborski SA, Swan M
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Growth of human stem cell-derived neurons on solid three dimensional polymers

Hayman MW, Smith KH, Cameron NR, Przyborski SA
Journal of Biochemical and Biophysical Methods (2005), 62, 231-240
31 March 2005
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Enhanced neurite outgrowth by human neurons grown on solid three-dimensional scaffolds

Hayman MW, Smith KH, Cameron NR, Przyborski SA
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