Alvetex Publications: 3D Cell Culture

Application of biomimetic surfaces and 3D culture technology to study the role of extracellular matrix interactions in neurite outgrowth and inhibition

K.E.Goncalves, S.Phillips, D.S.H.Shah, D.Athey, S.A.Przyborski
Biomaterials Advances
23 November 2022
DOI: 10.1016/j.bioadv.2022.213204

Investigation into the effect of skin tone modulators and exogenous stress on skin pigmentation utilizing a novel bioengineered skin equivalent

Kirsty Goncalves, Paola De Los Santos Gomez, Lydia Costello, Lucy Smith, Hugh Mead, Amy Simpson, Stefan Przyborski
Bioengineering and Translational Medicine
26 September 2022
DOI: 10.1002/btm2.10415

Early functional mismatch between breast cancer cells and their tumour microenvironment suppresses long term growth

Anna Perdrix Rosell, Oscar Maiques, Joshua Alexander James Martin, Probir Chakravarty, Luigi Ombrato, Victoria Sanz-Moreno, Ilaria Malanchi
Cancer Letters
28 September 2022
DOI: 10.1016/j.canlet.2022.215800

Macrophage PD-1 associates with neutrophilia and reduced bacterial killing in early cystic fibrosis airway disease

Camilla Margaroli, Hamed Horati, Luke W.Garratt, Vincent D. Giacalone, Craig Schofield, A. Susanne Dittrich, Tim Rosenow, Brian S.Dobosh, Hong S.Lim, Dario L.Frey, Mieke Veltman, George L.Silva, Milton R.Brown, Carsten Schultz, Harm A.W.M.Tiddens, Sarath Ranganathang, Joshua D.Chandler, Peng Qiu, Limin Peng, Bob J.Scholte, Marcus A.Mall, Anthony Kicic, Lokesh Guglani, Stephen M.Stick, Hettie M.Janssens, Rabindra Tirouvanziam
Journal of Cystic Fibrosis
19 June 2022
DOI: 10.1016/j.jcf.2022.06.001

Modelling liver cancer microenvironment using a novel 3D culture system

Ala’a Al Hrout, Karla Cervantes-Gracia, Richard Chahwan & Amr Amin
Scientific Reports 12, Article number: 8003 (2022)
14 May 2022
DOI: 10.1038/s41598-022-11641-7

Recreating Tissue Structures Representative of Teratomas In Vitro Using a Combination of 3D Cell Culture Technology and Human Embryonic Stem Cells

Alejandro Hidalgo Aguilar, Lucy Smith, Dominic Owens, Rebecca Quelch, and Stefan Przyborski
Bioengineering 2022, 9(5), 185
22 April 2022
DOI: 10.3390/bioengineering9050185

In vitro toxicity of glyphosate in Atlantic salmon evaluated with a 3D hepatocyte-kidney co-culture model

L.Søfteland and P.A.Olsvik
Food and Chemical Toxicology, Volumn 164
14 April 2022
DOI: 10.1016/j.fct.2022.113012

Biomimetic Full-Thickness Skin-on-a-Chip Based on a Fibroblast-Derived Matrix

Patrícia Zoio, Sara Lopes-Ventura, and Abel Oliva
Micro (MDPI)
12 March 2022
DOI: 10.3390/micro2010013

A Novel Coculture System for Assessing Respiratory Sensitizing Potential by IL-4 in T Cells (PDF)

Izuru Mizoguchi, Yasuhiro Katahira, Shinya Inoue, Eri Sakamoto, Aruma Watanabe, Yuma Furusaka, Atsushi Irie, Satoru Senju, Yasuharu Nishimura, Shusaku Mizukami, Kenji Hirayama, Sou Nakamura, Koji Eto, Hideaki Hasegawa and Takayuki Yoshimoto
ALTEX
24 February 2022
DOI: 10.14573/altex.2111181

Radiation exposure elicits a neutrophil-driven response in healthy lung tissue that enhances metastatic colonization

Emma Nolan, Victoria Louise Bridgeman, Luigi Ombrato, Adam Karoutas, Nicolas Rabas, Celine Angeli Natascha Sewneth, Marcos Vasquez, Felipe Silva Rodrigues, Stuart Horswell, Peter Faull, Rebecca Carter & Ilaria Malanchi
Nat Cancer 3, 173–187 (2022)
24 February 2022
DOI: 10.1038/s43018-022-00336-7

Neutrophil-derived extracellular vesicles promote feed-forward inflammasome signaling in cystic fibrosis airways

Osric A Forrest, Brian Dobosh, Sarah A Ingersoll, Sanjana Rao, Alexander Rojas, Julie Laval, Jessica A Alvarez, Milton R Brown, Vin Tangpricha, Rabindra Tirouvanziam
J Leuk Biol
16 February 2022
DOI: 10.1002/JLB.3AB0321-149R

Transcriptomic and Functional Evidence Show Similarities between Human Amniotic Epithelial StemCells and Keratinocytes

Li-Ping Liu, Dong-Xu Zheng, Zheng-Fang Xu, Hu-Cheng Zhou, Yun-Cong Wang, Hang Zhou, Jian-Yun Ge, Daisuke Sako, Mi Li, Kazunori Akimoto, Yu-Mei Li, and Yun-Wen Zheng
Cells 2022, 11(1), 70
27 December 2021
DOI: 10.3390/cells11010070

miR-378d suppresses malignant phenotype of ESCC cells through AKT signaling

Jie Peng, Susu Shi, Juan Yu, Jianli Liu, Haixiang Wei, Haixia Song, Shaoqiang Wang, Zhejie Li, Shujin He, Lei Li, Hongyan Zhang, Zhizhen Yan, Ran Zhao, Yukun Liu, Yanrong Liu, Junjun Li, Renya Zhang and Wei Wang
Cancer Cell International volume 21, Article number: 702 (2021)
22 December 2021
DOI: 10.1186/s12935-021-02403-y

Mass production of human airway-like neutrophils via transmigration in an organotypic model of human airways

Brian Dobosh, Vincent D Giacalone, Camilla Margaroli, Rabindra Tirouvanziam
Star Protocols. Volume 2, Issue 4
17 December 2021
DOI: 10.1016/j.xpro.2021.100892

Mechanistic analysis and significance of sphingomyelinasemediated decreases in transepithelial CFTR currents in nHBEs

Kirsten A Cottrill, Vincent D Giacalone, Camilla Margaroli, Robert J Bridges, Michael Koval, Rabindra Tirouvanziam, Nael A McCarty
Physiological Reports.
September 2021
DOI: 10.14814/phy2.15023

Derivation of Adult Human Cortical Organotypic Slice Cultures for Coculture with Reprogrammed Neuronal Cells

Giedre Kvist
Methods in Molecular Biology (MIMB), volume 2352
30 July 2021
DOI: 10.1007/978-1-0716-1601-7_17

Barrier-on-a-Chip with a Modular Architecture and Integrated Sensors for Real-Time Measurement of Biological Barrier Function

Patrícia Zoio, Sara Lopes-Ventura and Abel Oliva
Micromachines 2021, 12(7), 816
12 July 2021
DOI: 10.3390/mi12070816

Pigmented full-thickness human skin model based on a fibroblast-derived matrix for long-term studies

Mrs Patrícia Zoio, Mrs Sara Ventura, Mrs Mafalda Leite, and Dr Abel Oliva
Tissue Engineering Part C: Methods
19 June 2021
DOI: 10.1089/ten.TEC.2021.0069

miR-378d regulates polyploidization and malignant phenotype of tumor cells through AKT and RhoA

Jie Peng, Susu Shi, Juan Yu, Jianli Liu, Haixiang Wei, Haixia Song, Shaoqiang Wang, Daolu Guo, Zhejie Li, Shujin He, Lei Li, Hongyan Zhang, Zhizhen Yan, Ran Zhao, Yukun Liu, Yanrong Liu, Junjun Li, Renya Zhang, Wei Wang
Preprints 2021, 2021060443
16 June 2021
DOI: 10.20944/preprints202106.0443.v1

Immune modulatory effects of Idelalisib in stromal cells of chronic lymphocytic leukemia

Sarah Handl, Franziska von Heydebrand, Simon Voelkl, Robert A J Oostendorp, Jochen Wilke, Anita N Kremer, Andreas Mackensen, and Gloria Lutzny-Geier
Leukemia and Lymphoma
17 May 2021
DOI: 10.1080/10428194.2021.1927019

Using Advanced Cell Culture Techniques to Differentiate Pluripotent Stem Cells and Recreate Tissue Structures Representative of Teratoma Xenografts

L A Smith, A Hidalgo Aguilar, D D G Owens, R H Quelch, E Knight and S A Przyborski
Frontiers in Cell Development and Biology
06 May 2021
DOI: 10.3389/fcell.2021.667246

Extracellular Vesicles From a Three-Dimensional Culture of Perivascular Cells Accelerate Skin Wound Healing in a Rat

Min Ho Kim, Changho Chung, Mun Ho Oh, Jin Hyun Jun, Yong Ko and Jong Hun Lee
Aesthetic Plastic Surgery
05 April 2021
DOI: 10.1007/s00266-021-02254-y

SIRT1 Inhibits Apoptosis by Promoting Autophagic Flux in Human Nucleus Pulposus Cells in the Key Stage of Degeneration via ERK Signal Pathway

Fei He, Qingshu Li, Bo Sheng, Haitao Yang, and Wei Jiang
BioMed Research International, vol. 2021, Article ID 8818713.
DOI: 10.1155/2021/8818713

Use of Porous Polystyrene Scaffolds to Bioengineer Human Epithelial Tissues In Vitro

Costello L, Darling N, Freer M, Bradbury S, Mobbs C, Przyborski S
Methods in Molecular Biology (Clifton, N.J.)
1 January 2021
DOI: 10.1007/978-1-0716-1246-0_20

Generation of Keratinocytes from Human Induced Pluripotent Stem Cells Under Defined Culture Conditions

Shyam Kishor Sah, Jitendra K Kanaujiya, I-Ping Chen, and Ernst J Reichenberger
Cellular Reprogramming
29 December 2020
DOI: 10.1089/cell.2020.0046

The human bone marrow harbors a CD45⁻CD11B⁺ cell progenitor permitting rapid microglia-like cell derivative approaches

Andreas Bruzelius Isabel Hidalgo Antonio Boza-Serrano Anna-Giorgia Hjelmér Amelie Tison Tomas Deierborg Johan Bengzon Tania Ramos-Moreno
Stem Cells Translational Medicine
09 December 2020
DOI: 10.1002/sctm.20-0127

Exposure of the SH-SY5Y Human Neuroblastoma Cells to 50-Hz Magnetic Field: Comparison Between Two-Dimensional (2D) and Three-Dimensional (3D) In Vitro Cultures

Claudia Consales, Alessio Butera, Caterina Merla, Emanuela Pasquali, Vanni Lopresto, Rosanna Pinto, Maria Pierdomenico, Mariateresa Mancuso, Carmela Marino and Barbara Benassi
Molecular Neurobiology
24 November 2020
DOI: 10.1007/s12035-020-02192-x

Developing a novel 3D model of the intestinal epithelium as a tool to study the pharmacokinetics of new chemical entities

Matthew A Freer
Doctoral Thesis, Durham University, UK
25 September 2020

Bioengineering Novel in vitro Co-culture Models That Represent the Human Intestinal Mucosa With Improved Caco-2 Structure and Barrier Function

Nicole J Darling, Claire L Mobbs, Ariana L González-Hau, Matthew Freer and Stefan Przyborski
Frontiers in Bioengineering and Biotechnology
31 August 2020
DOI: 10.3389/fbioe.2020.00992

E7 oncoprotein from human papillomavirus 16 alters claudins expression and the sealing of epithelial tight junctions

Perla Yaceli Uc, Jael Miranda, Arturo Raya-Sandino, Lourdes Alarcón, María Luisa, Roldán Rodolfo, Ocadiz-Delgado, Enoc Mariano Cortés-Malagón, Bibiana Chávez-Munguía, Georgina Ramírez, René Asomoza, Liora Shoshani, Patricio Gariglio, Lorenza González-Mariscal
International Journal of Oncology
29 July 2020
DOI: 10.3892/ijo.2020.5105

Spheroid Trapping and Calcium Spike Estimation Techniques toward Automation of 3D Culture

Kenneth Ndyabawe, Mark Haidekker, Amish Asthana, William S Kisaalita
SLAS Technology
16 July 2020
DOI: 10.1177/2472630320938319

Grafted human pluripotent stem cell-derived cortical neurons integrate into adult human cortical neural circuitry

Marita Grønning Hansen Cecilia Laterza Sara Palma-Tortosa Giedre Kvist Emanuela Monni Oleg Tsupykov Daniel Tornero Naomi Uoshima Jordi Soriano Johan Bengzon Gianvito Martino Galyna Skibo Olle Lindvall Zaal Kokaia
Stem Cells — Translational Medicine
29 June 2020
DOI: 10.1002/sctm.20-0134

Effect of donor age and 3D-cultivation on osteogenic differentiation capacity of adipose-derived mesenchymal stem cells

Stephan Payr, Tim Schuseil, Marina Unger, Claudine Seeliger, Thomas Tiefenboeck, Elizabeth R Balmayor and Martijn van Griensven
Nature – Scientific Reports 10, Article number: 10408 (2020)
26 June 2020
DOI: 10.1038/s41598-020-67254-5

Recent developments of 3D models of the tumor microenvironment for cutaneous melanoma: Bridging the gap between the bench and the bedside?

Johanna Maria Hölken and Nicole Elisabeth Teusch
Microorganisms Volume 7: 1-7
7 May 2020
DOI: 10.15761/JTS.1000388

Novel 2D and 3D Assays to Determine the Activity of Anti-Leishmanial Drugs

Alec O’Keeffe, Christine Hale, James A. Cotton, Vanessa Yardley, Kapish Gupta, Abhishek Ananthanarayanan, Sudaxshina Murdan, and Simon L Croft
Microorganisms 2020, 8(6), 831
1 June 2020
DOI: 10.3390/microorganisms8060831

Modelling human CNS injury with human neural stem cells in 2- and 3-Dimensional cultures

Barbora Vagaska, Olivia Gillham and Patrizia Ferretti
Scientific Reports 10, Article number: 6785(2020)
22 April 2020
DOI: 10.1038/s41598-020-62906-y

Applications of novel bioreactor technology to enhance the viability and function of cultured cells and tissues

H W Hoyle, L A Smith, R J Williams and S A Przyborski
The Royal Society — Interface Focus
14 February 2020
DOI: 10.1098/rsfs.2019.0090

Apelin Promotes ECM Synthesis by Enhancing Autophagy Flux via TFEB in Human Degenerative NP Cells under Oxidative Stress

Wei Jiang, Pei Zhao, and Xuemei Zhang
Biomed Research Journal
14 February 2020
DOI: 10.1155/2020/4897170

Dataset supporting “Multiple scattering in scanning helium microscopy”

Lambrick S, Vozdecky L, Bergin M, Halpin J E, MacLaren D A, Dastoor P C, Przyborski S A, et al.
University of Cambridge, Apollo Repository
31 January 2020
DOI: 10.17863/CAM.48316

Micropatterning Method for Porous Materials Using the Difference of the Glass Transition Temperature between Exposed and Unexposed Areas of a Thick-Photoresist

Hidetaka Ueno, Kiichi Sato, Kou Yamada and Takaaki Suzuki
Micromachines
2020. 11(1), 54.
DOI: 10.3390/mi11010054

Could 3D models of cancer enhance drug screening?

Virginia Brancato, Joaquim Miguel Oliveira, Vitor Manuel Correlo, Rui Luis Reis, Subhas C.Kunduab
Biomaterials
26 December 2019.
DOI: 10.1016/j.biomaterials.2019.119744

Metastatic-niche labelling reveals parenchymal cells with stem features

Luigi Ombrato, Emma Nolan, Ivana Kurelac, Antranik Mavousian, Victoria Louise Bridgeman, Ivonne Heinze, Probir Chakravarty, Stuart Horswell, Estela Gonzalez-Gualda, Giulia Matacchione, Anne Weston, Joanna Kirkpatrick, Ehab Husain, Valerie Speirs, Lucy Collinson, Alessandro Ori, Joo-Hyeon Lee and Ilaria Malanchi
Nature
volume 572, pages 603-608 (2019)
DOI: 10.1038/s41586-019-1487-6

Emulsion templated scaffolds manufactured from photocurable polycaprolactone

Betül Aldemir Dikici, Colin Sherborne, Gwendolen C.Reilly, Frederik Claeyssens
Science Direct
26 June 2019
DOI: 10.1016/j.polymer.2019.05.023

Engineering a Multilayered Skin Equivalent: The Importance of Endogenous Extracellular Matrix Maturation to Provide Robustness and Reproducibility

Lydia Costello, Nicola Fullard, Mathilde Roger, Steven Bradbury, Teresa Dicolandrea, Robert Isfort, Charles Bascom, Stefan Przyborski
Skin Tissue Engineering
31 May 2019
DOI: 10.1007/978-1-4939-9473-1_9

Osteogenic differentiation of human adipose-derived stem cells in 3D conditions - comparison of spheroids and polystyrene scaffolds

Rumiński S, Kalaszczyńska I, Długosz A, Lewandowska-Szumieł M.
Eur Cell Mater
17 May 2019
DOI: 10.22203/eCM.v037a23

CAFs and TGF-β Signaling Activation by Mast Cells Contribute to Resistance to Gemcitabine/Nabpaclitaxel in Pancreatic Cancer

Letizia Porcelli, Rosa Maria Iacobazzi, Roberta Di Fonte, Simona Serratì, Angelica Intini, Antonio Giovanni Solimando, Oronzo Brunetti, Angela Calabrese, Francesco Leonetti, Amalia Azzariti and Nicola Silvestris
Cancers 2019, 11, 330
7 March 2019
DOI: 10.3390/cancers11030330

Neutrophil Dysfunction in the Airways of Children with Acute Respiratory Failure Due to Lower Respiratory Tract Viral and Bacterial Coinfections

Jocelyn R Grunwell, Vincent D Giacalone, Susan Stephenson, Camilla Margaroli, Brian S Dobosh, Milton R Brown, Anne M Fitzpatrick and Rabindra Tirouvanziam
Nature – Scientific Reports 9, Article number: 2874 (2019)
27 February 2019
DOI: 10.1038/s41598-019-39726-w

Bioengineering the microanatomy of human skin

Mathilde Roger, Nicola Fullard, Lydia Costello, Steven Bradbury, Ewa Markiewicz, Steven O'Reilly, Nicole Darling, Pamela Ritchie, Arto Määttä, Iakowos Karakesisoglou, Glyn Nelson, Thomas von Zglinicki, Teresa Dicolandrea, Robert Isfort, Charles Bascom, Stefan Przyborski
Journal of Anatomy
10 February 2019
DOI: 10.1111/joa.12942

A multifunctional drug nanocarrier for efficient anticancer therapy

Carmen Teijeiro-Valiño, Ramon Novoa-Carballal, Erea Borrajo, Anxo Vidal, Marta Alonso-Nocelo, Maríade la Fuente Freire, Pedro P Lopez-Casas, Manuel Hidalgo, Noémi Csaba, María José Alonso
Journal of Controlled Release.
28 January 2019.
DOI: 10.1016/j.jconrel.2018.12.002

Preparation of 3D porous hydroxyapatite cell scaffolds using polystyrene templates under biomimetic conditions

Kazutoshi IIJIMA, Ayako IIZUKA, Ryo SUZUKI, Hitomi UENO-YOKOHATA3, Nobutaka KIYOKAWA, Mineo HASHIZUME
Journal of the Ceramic Society of Japan 126 [11] 956-958 2018
November 2018
DOI: 10.2109/jcersj2.18144

Glioblastoma’s Next Top Model: novel culture systems for brain cancer radiotherapy research

Seamus P Caragher, Anthony J Chalmers MD/PhD, Natividad Gomez-Roman PhD
Preprints 2018, 2018100697
30 October 2018
DOI: 10.20944/preprints201810.0697.v1

Regulation of human glioma cell migration, tumor growth, and stemness gene expression using a Lck targeted inhibitor

J P Zepecki, K M Snyder, M M Moreno, E Fajardo, A Fiser, J Ness, A Sarkar, S A Toms and N Tapinos
Oncogene
23 October 2018
DOI: 10.1038/s41388-018-0546-z

Transcription factor programming of human ES cells generates functional neurons expressing both upper and deep layer cortical markers

Giedre Miskinyte, Marita Grønning Hansen, Emanuela Monni, Matti Lam, Johan Bengzon, Olle Lindvall, Henrik Ahlenius, Zaal Kokaia
ACS Omega 2018, 3 (8), pp 10180-10187
11 October 2018
DOI: 10.1371/journal.pone.0204688

Osteogenic Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells in Electrospun Silica Nonwoven Fabrics

Kazutoshi Iijima, Shohei Ishikawa, Kohei Sasaki, Mineo Hashizume, Masaaki Kawabe, and Hidenori Otsuka
ACS Omega 2018, 3 (8), pp 10180-10187
30 August 2018
DOI: 10.1021/acsomega.8b01139

The tyrosine phosphorylated pro-survival form of Fas intensifies the EGF-induced signal in colorectal cancer cells through the nuclear EGFR/STAT3-mediated pathway

Ngoc Ly Ta, Krittalak Chakrabandhu, Sébastien Huault and Anne-Odile Hueber
Scientific Reports volume 8, Article number: 12424 (2018)
20 August 2018
DOI:10.1038/s41598-018-30804-z

Host-pathogen-interaction reconstituted in 3-dimensional cocultures of mucosa and C. albicans

Mrs Romina Buchs, Mr Bruno Lehner, Dr Phillippe Meuwly, Prof Bruno Schnyder
Tissue Engineering Part C: Methods
14 June 2018
DOI: 10.1089/ten.TEC.2018.0042

Frontline Science: Pathological conditioning of human neutrophils recruited to the airway milieu in cystic fibrosis

Osric A Forrest, Sarah A Ingersoll, Marcela K Preininger, Julie Laval, Dominique H Limoli, Milton R Brown, Frances E Lee, Brahmchetna Bedi, Ruxana T Sadikot, Joanna B Goldberg, Vin Tangpricha, Amit Gaggar, Rabindra Tirouvanziam
Journal of Leukocyte Biology
09 May 2018
DOI: 10.1002/JLB.5HI1117-454RR

Differentiation Patterns of Immortalized Human Meibomian Gland Epithelial Cells in Three-Dimensional Culture

Nagayoshi Asano, Ulrike Hampel, Fabian Garreis, Antje Schröder, Martin Schicht, Christian M Hammer, Friedrich Paulsen
Investigative Ophthalmology and Visual Science, Vol.59, 1343-1353
March 2018
DOI: 10.1167/iovs.17-23266

Development of a microfluidic perfusion 3-dimensional cell culture system

Dong Hyeok Park, Hyeong Jin Jeon, Moon Jeong Kim, Xuan Don Nguyen, Karl Morten and Jeung Sang Go
Journal of Micromechanics and Microengineering
17 January 2018
DOI: 10.1088/1361-6439/aaa877

Substrate Microarchitecture Shapes the Paracrine Crosstalk of Stem Cells with Endothelial Cells and Osteoblasts

Francisco Martín-Saavedra, Lara Crespo, Clara Escudero-Duch, Laura Saldaña, Enrique Gómez-Barrena and Nuria Vilaboa
Scientific Reports 7 Article number: 15182
9 November 2017
DOI: 10.1038/s41598-017-15036-x

Paracrine interactions between mesenchymal stem cells and macrophages are regulated by 1,25-dihydroxyvitamin D3

Laura Saldaña, Gema Vallés, Fátima Bensiamar, Francisco José Mancebo, Eduardo García-Rey and Nuria Vilaboa
Scientific Reports 7, Article number: 14618
3 November 2017
doi:10.1038/s41598-017-15217-8

Effect of_the Scaffold Microenvironment on Cell Polarizability and Capacitance Determined by Probabilistic Computations

Pazmino Betancourt B, Florczyk S, Simon M, Juba D, Douglas JF, Keyrouz W, Bajcsy P, Lee C, Simon CG
Biomedical Materials
26 October 2017
DOI: 10.1088/1748-605X/aa9650

Cale Dobrosak, Jonathan H Gooi
J. Bone Reports
16 October 2017
DOI: 10.1016/j.bonr.2017.10.002

Revisiting the Road Map of Medullary Thymic Epithelial Cell Differentiation

Michel C, Miller CN, Küchler R, Brors B, Anderson MS, Kyewski B, Pinto S
J Immunol. pii: ji1700203
9 October 2017
doi: 10.4049/jimmunol.1700203

Autocrine and Paracrine Regulation of the Murine Skeleton by Osteocyte-Derived Parathyroid Hormone-Related Protein

Ansari N, Ho PW, Crimeen-Irwin B, Poulton IJ, Brunt AR, Forwood MR, Divieti Pajevic P, Gooi JH, Martin TJ, Sims NA
J Bone Miner Res
15 September 2017
DOI: 10.1002/jbmr.3291

S Florczyk, M Simon, D Juba, S Pine, S Sarkar, D Chen, P Baker, S Bodhak, A Cardone, M Brady, P Bajcsy, C G Simon
ACS Biomater Sci Eng
31 August 2017
DOI: 10.1021/acsbiomaterials.7b00473

Galectin-1 from conditioned medium of three-dimensional culture of adipose-derived stem cells accelerates migration and proliferation of human keratinocytes and fibroblasts

MH Kim, WH Wu, JH Choi, JH Kim, JH Jun, Y Ko and JH Lee MD
Wound Repair and Regeneration
30 August 2017
DOI: 10.1111/wrr.12579

SHH signalling mediates astrocyte crosstalk with neurons to confer neuroprotection

Ugbode CI, Smith I, Whalley BJ, Hirst WD, Rattray M
J Neurochem
9 May 2017
doi: 10.1111/jnc.14064

Dedifferentiation into blastomere-like cancer stem cells via formation of polyploid giant cancer cells

Niu N, Mercado-Uribe I, Liu J
Oncogene
24 April 2017
DOI: 10.1038/onc.2017.72

Osteocyte secreted factors inhibit skeletal muscle differentiation

Wood CL, Divieti Pajevic P, Gooi JH
Bone Reports. p74-80.
6 June 2017
DOI: 10.1016/j.bonr.2017.02.007

Inhibition of glioblastoma dispersal by the MEK inhibitor PD0325901

Shannon S, Jia D, Entersz I, Beelen P, Yu M, Carcione C, Carcione J, Mahtabfar A, Vaca C, Weaver M, Shreiber D, Zahn JD, Liu L, Lin H, Foty RA
BMC Cancer. 17(1):121.
10 February 2017
doi: 10.1186/s12885-017-3107-x

A robust and reproducible human pluripotent stem cell derived model of neurite outgrowth in a three-dimensional culture system and its application to study neurite inhibition

Clarke KE, Tams DM, Henderson AP, Roger MF, Whiting A, Przyborski SA
Neurochem Int. pii: S0197-0186(16)30507-1
20 December 2016
DOI: 10.1016/j.neuint.2016.12.009

Synthesis of Near-Infrared Fluorescent Two-Photon-Absorbing Fluorenyl Benzothiadiazole and Benzoselenadiazole Derivatives

Sheng Yao, Bosung Kim, Xiling Yue, Maria Y. Colon Gomez, Mykhailo V. Bondar, and Kevin D. Belfield
ACS Omega, 2016, 1 (6), pp 1149-1156
7 December 2016
DOI: 10.1021/acsomega.6b00289

A novel 3D human glioblastoma cell culture system for modeling drug and radiation responses

Natividad Gomez-Roman, Katrina Stevenson, Lesley Gilmour, Graham Hamilton and Anthony J Chalmers
Neuro Oncology
30 August 2016
DOI: 10.1093/neuonc/now164

Bcl-xL is an oncogenic driver in colorectal cancer

Scherr AL, Gdynia G, Salou M, Radhakrishnan P, Duglova K, Heller A, Keim S, Kautz N, Jassowicz A, Elssner C, He YW, Jaeger D, Heikenwalder M, Schneider M, Weber A, Roth W, Schulze-Bergkamen H, Koehler BC
Cell Death Dis. 7(8):e2342
18 August 2016
DOI: 10.1038/cddis.2016.233

The dual role of asporin in breast cancer progression

Simkova D, Kharaishvili G, Korinkova G, Ozdian T, Suchánková-Kleplová T, Soukup T, Krupka M, Galandakova A, Dzubak P, Janikova M, Navratil J, Kahounova Z, Soucek K, Bouchal J
Oncotarget
7 July 2016
DOI: 10.18632/oncotarget.10471

TDP-43 regulates endogenous retrovirus-K viral protein accumulation

Manghera M, Ferguson-Parry J, Douville RN
Neurobiol Dis. 94:226-36
October 2016
DOI: 10.1016/j.nbd.2016.06.017

Influence of Matrices on 3D-Cultured Prostate Cancer Cells’ Drug Response and Expression of Drug-Action Associated Proteins

Edmondson R, Adcock AF, Yang L
Public Library of Science. PloS ONE 11(6):e0158116
28 June 2016
DOI: 10.1371/journal.pone.0158116

Structural Analysis of Three-dimensional Human Neural Tissue derived from Induced Pluripotent Stem Cells

Patrick Terrence Brooks, Mikkel Aabech Rasmussen and Poul Hyttel
J Stem Cell Res Ther 6:4
2016
DOI: 10.4172/2157-7633.1000337

Astrocytes Grown in Alvetex Three Dimensional Scaffolds Retain a Non-reactive Phenotype

Ugbode CI, Hirst WD, Rattray M
Neurochem Res. 41(8):1857-67
21 April 2016
doi: 10.1007/s11064-016-1911-3

3D Reconstruction of the Human Airway Mucosa In Vitro as an Experimental Model to Study NTHi Infections

Pasquale Marrazzo, Silvia Maccari, Annarita Taddei, Luke Bevan, John Telford, Marco Soriani, Alfredo Pezzicoli
Public Library of Science. PLoS ONE
April 21, 2016
DOI: 10.1371/journal.pone.0153985

Comparison of the Osteogenic Potential of Mineral Trioxide Aggregate and Endosequence Root Repair Material in a 3-dimensional Culture System

Rifaey HS, Villa M, Zhu Q, Wang YH, Safavi K, Chen IP
J Endod. 2016 42(5):760-5
16 March 2016
DOI: 10.1016/j.joen.2016.02.001

Preclinical evaluation of the AR inhibitor enzalutamide in triple-negative breast cancer cells

Caiazza F, Murray A, Madden SF, Synnott NC, Ryan EJ, O’Donovan N, Crown J, Duffy MJ
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James Jenkins, Ruslan I Dmitriev, Karl Morten, Kieran W McDermott, Dmitri B Papkovsky
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Novel Three Dimensional Human Endocervix Cultures respond to 28-day hormone treatment

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Capturing complex tumour biology in vitro: histological and molecular characterisation of precision cut slices

Emma J Davies, Meng Dong, Matthias Gutekunst, Katja Närhi, Hanneke JAA van Zoggel, Sami Blom, Ashwini Nagaraj, Tauno Metsalu, Eva Oswald, Sigrun Erkens-Schulze, Juan A Delgado San Martin, Riku Turkki, Stephen R Wedge, Taija M af Hällström, Julia Schueler, Wytske M van Weerden, Emmy W Verschuren, Simon T Barry, Heiko van der Kuip and John A Hickman
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Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Lara Stevanato, Caroline Hicks, John D. Sinden
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Heterogeneity between triple negative breast cancer cells due to differential activation of Wnt and PI3K/AKT pathways

Gabriela Martínez-Revollar, Erika Garay, Dolores Martin-Tapia, Porfirio Nava, Miriam Huerta, Esther Lopez-Bayghen, Noemí Meraz-Cruz, José Segovia, Lorenza González-Mariscal
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Neuronal-glial populations form functional networks in a biocompatible 3D scaffold

Imogen Smith, Marcus Haag, Christopher Ugbode, Daniel Tams, Marcus Rattray, Stefan Przyborski, Angela Bithell, Benjamin J Whalley
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A Novel Fully Humanized 3D Skin Equivalent to Model Early Melanoma Invasion

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Dexamethasone-Mediated Activation of Fibronectin Matrix Assembly Reduces Dispersal of Primary Human Glioblastoma Cells

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A method for the evaluation of thousands of automated 3D stem cell segmentations

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Two and three dimensional graphene substrates to magnify osteogenic differentiation of periodontal ligament stem cells

Han Xie, Tong Cao, José Viana Gomes, Antônio Hélio Castro Neto, Vinicius Rosa
Carbon. Volume 93
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The Wnt-inhibitor Sclerostin is Up-regulated by Mechanical Unloading in Osteocytes in vitro

Jordan M Spatz, Marc N Wein, Jonathan H Gooi, Yili Qu, Jenna L Garr, Shawn Liu, Kevin J Barry, Yuhei Uda, Forest Lai, Christopher Dedic, Mercedes Balcells-Camps, Henry M Kronenberg, Philip Babij, Paola Divieti Pajevic
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Increased survival and exocytosis of toxic primary granules by neutrophils in an in vitro model of Cystic Fibrosis airway disease (INC6P.310)

Osric Forrest, Sarah Ingersoll, Marcela Preininger, Julie Laval, Milton Brown and Rabindra Tirouvanziam
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Targeting ADAM-17 with an inhibitory monoclonal antibody has antitumour effects in triple negative breast cancer cells

F Caiazza, P M McGowan, M Mullooly, A Murray, N Synnott, N O’Donovan, L Flanagan, C J Tape, G Murphy, J Crown and M J Duffy
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26 May 2015
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Human neural stem cell-derived cultures in three-dimensional substrates form spontaneously functional neuronal networks

Imogen Smith, Vasco Silveirinha, Jason L Stein, Luis de la Torre-Ubieta, Jonathan A Farrimond, Elizabeth M Williamson and Benjamin J Whalley
Journal of Tissue Engineering and Regenerative Medicine
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Assessing Na⁺/H⁺exchange and cell effector functionality in metastatic breast cancer

Schammim Ray Amith, Jodi Marie Wilkinson, Larry Fliegel
J Biochimie Open. 6. 2 (2016) 16-23
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Imaging oxygen in neural cell and tissue models by means of anionic cell-permeable phosphorescent nanoparticles

Ruslan I Dmitriev, Sergey M Borisov, Alina V Kondrashina, Janelle MP Pakan, Ujval Anilkumar, Jochen H M Prehn, Alexander V Zhdanov, Kieran W McDermott, Ingo Klimant, Dmitri B Papkovsky
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Development of positive control tissue for in situ hybridisation using Alvetex scaffolds

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Topographical cues regulate the crosstalk between MSCs and macrophages

Gema Vallés, Fátima Bensiamar, Lara Crespo, Manuel Arruebo, Nuria Vilaboa, Laura Saldaña
Biomaterials
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Pan-Bcl-2 Inhibitor Obatoclax Delays Cell Cycle Progression and Blocks Migration of Colorectal Cancer Cells

Bruno Christian Koehler, Anna-Lena Scherr, Stephan Lorenz, Christin Elssner, Nicole Kautz, Stefan Welte, Dirk Jaeger, Toni Urbanik, Henning Schulze-Bergkamen
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Chondrogenic potential of human articular chondrocytes and skeletal stem cells: A comparative study

Siwei Li, Bram G Sengers, Richard OC Oreffo and Rahul S Tare
Journal of Biomaterials Applications
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Morphology and functions of astrocytes cultured on water-repellent fractal tripalmitin surfaces

Wei-wei Hu, Zhe Wang, Shan-shan Zhang, Lei Jiang, Jing Zhang, Xiangnan Zhang, Qun-fang Lei, Hyun-Joo Park, Wen-jun Fang, Zhong Chen
Biomaterials 2014
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The role of high cell density in the promotion of neuroendocrine transdifferentiation of prostate cancer cells

Zuzana Pernicová, Eva Slabáková, Radek Fedr, Šárka Šimečková, Josef Jaroš, Tereza Suchánková, Jan Bouchal, Gvantsa Kharaishvili, Milan Král, Alois Kozubík and Karel Souček
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The effects of microRNAs on human neural stem cell differentiation in two- and three-dimensional cultures

Lara Stevanato and John D Sinden
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Small molecule phosphorescent probes for O₂ imaging in 3D tissue models

Ruslan I Dmitriev, Alina V Kondrashina, Klaus Koren, Ingo Klimant, Alexander V Zhdanov, Janelle MP Pakan, Kieran W McDermott and Dmitri B Papkovsky
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Hanging drop: An in vitro air toxic exposure model using human lung cells in 2D and 3D structures

Faye F Liu, Cheng Peng, Beate I Escher, Emmanuelle Fantino, Cindy Giles, Stephen Were, Lesley Duffy, Jack C. Ng
Journal of Hazardous Materials
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Beyond Cell Death - Antiapoptotic Bcl-2 Proteins Regulate Migration and Invasion of Colorectal Cancer Cells In Vitro

Bruno Christian Koehler, Anna-Lena Scherr, Stephan Lorenz, Toni Urbanik, Nicole Kautz, Christin Elssner, Stefan Welte, Justo Lorenzo Bermejo, Dirk Jäger, Henning Schulze-Bergkamen
Public Library of Science. PLoS ONE 8(10): e76446
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Effect of PEG Surface Conformation on Anticancer Activity and Blood Circulation of Nanoemulsions Loaded with Tocotrienol-Rich Fraction of Palm Oil

Alaadin Alayoubi, Saeed Alqahtani, Amal Kaddoumi, Sami Nazzal
The AAPS Journal
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Overlapping gene coexpression patterns in human medullary thymic epithelial cells generate self-antigen diversity

Sheena Pinto, Chloé Michela, Hannah Schmidt-Glenewinkel, Nathalie Harder, Karl Rohr, Stefan Wild, Benedikt Brorse, and Bruno Kyewskia
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Epidermal-like architecture obtained from equine keratinocytes in three-dimensional cultures

Ruchi Sharma, Safia Z Barakzai, Sarah E Taylor, F Xavier Donadeu
Journal of Tissue Engineering and Regenerative Medicine
30 July 2013
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Targeting of Beta Adrenergic Receptors Results in Therapeutic Efficacy against Models of Hemangioendothelioma and Angiosarcoma

Jessica M Stiles, Clarissa Amaya, Steven Rains, Dolores Diaz, Robert Pham, James Battiste, Jaime F Modiano, Victor Kokta, Laura E Boucheron, Dianne C Mitchell, Brad A Bryan
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Characterization of liver specific functions of upcyte® hepatocytes grown in 3D and co-cultured with upcyte® endothelial cells

Dähn C, Hewitt N, Maltman D, Talas G, Przyborski S, Heinz S, Nörenberg A, Scheller K, Braspenning J
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Generation of proliferating human hepatocytes using upcyte® technology: characterisation and applications in induction and cytotoxicity assays

Alexandra Burkard, Caroline Dähn, Stefan Heinz, Anne Zutavern, Vera Sonntag-Buck, Daniel Maltman, Stefan Przyborski, Nicola J. Hewitt, and Joris Braspenning
Xenobiotica
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The autophagy-associated factors DRAM1 and p62 regulate cell migration and invasion in glioblastoma stem cells

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Rat primary hepatocytes show enhanced performance and sensitivity to acetaminophen during three-dimensional culture on a polystyrene scaffold designed for routine use

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Dysregulated TRK signalling is a therapeutic target in CYLD defective tumours

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Adipose tissue-derived stem cells display a proangiogenic phenotype on 3D scaffolds.

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Alvetex: Polystyrene scaffold technology for routine three dimensional cell culture.

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Validation of reference gene stability for APAP hepatotoxicity studies in different in vitro systems and identification of novel potential toxicity biomarkers

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Developments in three dimensional cell culture technology aimed at improving the accuracy of in vitro analyses

Maltman D, Przyborski SA
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Emulsion-templated porous scaffolds enabling three dimensional cell culture. Polymer Preprints

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Culture of HepG2 liver cells on three dimensional polystyrene scaffolds enhances cell structure and function during toxicological challenge

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Effect of synthesis parameters on emulsion-templated porous polymer formation and evaluation for 3D cell culture scaffolds

Bokhari M, Carnachan R, Przyborski SA, Cameron NR
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Novel cell culture device enabling three-dimensional cell growth and improved cell function

Bokhari M, Carnachan R, Cameron NR, Przyborski, SA
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Tailoring the morphology of emulsion-templated porous polymers

Carnachan RJ, Bokhari M, Przyborski SA, Cameron NR
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Porous polymers by emulsion templating

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Growth of human stem cell-derived neurons on solid three dimensional polymers

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Enhanced neurite outgrowth by human neurons grown on solid three-dimensional scaffolds

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