Image: One of our scientists dissecting subcutaneous resistance arteries.
Why Choose Isolated Arteries for Vascular Safety Assessment?
- Proven Methodology: The most significant discoveries about vascular biology, particularly the endothelium and smooth muscle, have come from studies using isolated arteries and arterioles. These studies have advanced our understanding of key neurohormonal regulators like nitric oxide (NO), endothelin, and endothelium-derived hyperpolarizing factor (EDHF).
- Human and Animal Models: While animal studies remain vital, they don't fully capture human vascular biology. Our assays offer the unique advantage of using isolated human tissues, providing more accurate predictions of drug effects in humans. Human tissue obtained from operating theatres allows direct comparisons with animal tissue, giving valuable insights into therapeutic drug activity.
- Regulatory Compliance: The data generated from our assays can be used in regulatory submissions to assess the propensity of drugs to cause vasoconstriction or vasodilatation. We offer studies conducted in accordance with Good Laboratory Practice (GLP) regulations, ensuring the highest standards of data integrity.
Key Features of Our Subcutaneous Arteries Assay
- Detailed Vascular Assessment: We measure drug-induced vasoconstriction and vasodilatation to evaluate the potential impact on blood pressure. Our assays are designed to mimic in vivo conditions as closely as possible, ensuring that in vitro results are predictive of in vivo outcomes.
- Comprehensive Comparisons: Our assays allow for direct comparison with reference vasodilators and vasoconstrictors, providing a thorough evaluation of your test articles.
- Advanced Techniques: We use cutting-edge technologies, such as wire myographs, to measure changes in artery tone under isometric conditions. This setup enables precise measurements of micro-Newton changes in force, reflecting active vasoconstriction or relaxation of the artery.
Customizable Protocols and Experimental Set-up
Understanding that every research project has unique needs, we offer customized protocols tailored to your specific requirements. Whether you need a particular assay configuration or additional analysis, our expert scientists are ready to collaborate with you to ensure your study's success.
Image: The myograph consists of two stainless steel “jaws”. One jaw is connected to a moveable micrometer, the other to a highly sensitive force transducer capable of measuring micro-Newton changes in force.
