Products: SynFire Induced Neurons

SynFire is a brand name for iPSC-derived Neurons

SynFire neurons from Neucyte Labs are pure and ready-to-use iPSC-derived glutamatergic or GABAergic induced neurons (iNs) and astroglia. This platform most closely resembles real human neurobiology observed in primary cultures, providing the ability to effectively study the function of human neurons in vitro.

Advantages of SynFire iNs

Real Human Biology – These cells more closely resemble real human biology than alternatives such as common animal models or other iPSC-based systems. SynFire iNs provide better suitability to predict responses to test agents.

Rapid and Homogeneous Maturation – SynFire iNs develop mature synaptic network activity within 3-4 weeks. These iNs show synchronous bursting phenotypes similar to rodent primary cultures.

Reliable, Robust, and Ready-to-Use Cultures – This differentiation approach results in a highly defined in vitro system with lot-to-lot consistency that provides reproducible results.

Flexible Modular System – The user can control subtype seeding densities and ratios to customize cultures to specific projects. Custom kit configurations are available.

Applications of SynFire iNs

Drug Discovery

In vitro neural disease modeling
Neuronal cell-based assays
Targeted phenotypic drug screening
Neural subtype-specific biochemistry
Target ID and validation in biologically relevant tissue

CNS Safety / Neurotoxicity

Neural cytotoxicity (apoptosis, mitochondrial activity, etc.)
Cell Stress tests
Neural network physiology assessment (MEA)
Seizurogenic potential testing
Neurite outgrowth and morphology evaluation
Mechanism of Action prediction by gene expression profiling

Product Selection Guide

Cat. No.	Procuct Name	Glutamatergic Induced Neurons	GABAergic Induced Neurons	Astroglia	Media
NC1002-10	SynFire GABAergic Induced Neuron Kit		✔	✔	✔
NC1001-10	SynFire Glutamatergic Induced Neuron Kit	✔		✔	✔
NC1010-1.5	Synfire Induced Neuron Co-Culture Kit	✔	✔	✔	✔
NC2010-10	SynFire Induced Neuron Media Kit				✔

SynFire GABAergic Induced Neuron Kit View product

Co-Culture kits containing GABAergic induced neurons, astroglia, and all of the media necessary to establish a co-culture model for functional neuron studies. Available in two sizes, small (Cat. No. NC1001-10) and large (Cat. No. NC1001-50).	NC1002-10	1 kit (small)
	NC1002-20	1 vial (1.5 million cells)
	NC1002-40	1 kit (large)
	NC1002-50	1 vial (3.5 million cells)

SynFire Glutamatergic Induced Neuron Kit View product

Co-culture kits containing glutamatergic induced neurons, astroglia, and all of the media necessary to establish a co-culture model for functional neuron studies. Available in two sizes, small (Cat. No. NC1001-10) and large (Cat. No. NC1001-50).	NC1001-10	1 kit (small)
	NC1001-20	1 vial (1.5 million cells)
	NC1001-50	1 kit (large)
	NC1001-60	1 vial (3.5 million cells)

SynFire Induced Neuron Co-Culture Kit View product

Glutamatergic and GABAergic induced neuron co-culture kits (with astrocytes) are available in two configurations suitable for general culture (Cat. No. NC1010-1.5) or MEA assays (Cat. No. NC1010-7.5). Please see the specification for kit contents.	NC1010-1.5	1 basic kit
	NC1010-7.5	1 MEA kit

SynFire Induced Neuron Media View product

Cat. No. NC2010-10 SynFire Complete Media Kit (small)
— Seeding (10 mL), short-term (20 mL), and long-term (60 mL) media and supplements.

NC2010-10

1 kit (small)

Cat. No. NC2010-20 SynFire Complete Media Kit (large)
— Seeding (20 mL), short-term (40 mL), and long-term (120 mL) media and supplements.

NC2010-20

1 kit (large)

Cat. No. NC2003-1 SynFire Media (for long term maintenance)
— Long term media (120 mL) for extended culture.

These media kits provide additional media (over what is included in the neuron kit) to support the culture of GABAergic (Cat. No. NC1002-10), glutamatergic (Cat. No. NC1001-10), and mixed induced neurons (Cat. No. NC1010-1.5). Custom media configurations are also available: please inquire at info-us@reprocell.com.

To support custom plating conditions or extended culture, additional SynFire media is available. See specifications for kit components.

NC2003-1

1 kit (LT)