SynFire® GABAergic Induced Neuron Kit

SynFire iN (Induced Neurons) cells are suitable for a variety of biochemical assays. For example, the effect of compounds on neuronal survival, axonal outgrowth, or dendritic arborization can be measured by standard assessment of viability, or image-based analysis of labeled cells, respectively. When co-cultured with glial cells, effects on synapse formation and composition, transcriptional programs, and electrophysiology can be tested. Neuronal subtypes can be mixed in different ratios for making a defined co-culture for different experimental purposes.

Specifications

Kit Contents

 SynFire GABAergic Induced Neuron Kit (Small)    (Cat. No. NC1002-10)

• Part No. 1002-1.5: GABAergic induced neurons (>1.5x10⁶ cells/vial)
• Part No. 1003-1.5: Astroglia (>1.5x10⁶ cells/vial)
• Part No. 2001-10: Seeding Basal Media (Enough for 10 mL)
• Part No. 2001S-10: Seeding Supplement (Enough for 10 mL)
• Part No. 2002-20: Short-term Basal Media (Enough for 20 mL)
• Part No. 2002S-20: Short-term Supplement (Enough for 20 mL)
• Part No. 2003-60: Long-term Basal Media (Enough for 60 mL)
• Part No. 2003S-60: Long-term Supplement (Enough for 60 mL)

 SynFire GABAergic Induced Neuron Kit (Large)    (Cat. No. NC1002-50)

• Part No. 1002-3.5: GABAergic induced neurons (>3.5x10⁶ cells/vial)
• Part No. 1003-3.5: Astroglia (>3.5x10⁶ cells/vial)
• Part No. 2001-20: Seeding Basal Media (Enough for 20 mL)
• Part No. 2001S-20: Seeding Supplement (Enough for 20 mL)
• Part No. 2002-40: Short-term Basal Media (Enough for 40 mL)
• Part No. 2002S-40: Short-term Supplement (Enough for 40 mL)
• Part No. 2003-120: Long-term Basal Media (Enough for 120 mL)
• Part No. 2003S-120: Long-term Supplement (Enough for 120 mL)

SynFire GABAergic Induced Neurons(Small) (Cat. No. NC1002-20) Part No. 1002-1.5: GABAergic induced neurons (>1.5x10⁶ cells/vial)

 SynFire GABAergic Induced Neurons(Large) (Cat. No. NC1002-60) Part No. 1002-3.5: GABAergic induced neurons (>3.5x10⁶cells/vial)

Storage and Stability

Store in cells in liquid nitrogen (Astroglia) or -80°C freezer (iNs) immediately upon receipt. Store other reagents at temperature indicated on the label.

This product is stable for at least 6 months from the date of receiving when stored as directed.

QC

Functional validation of maturation and neural network formation potential of iPSC derived induced neurons has been done on MEAs. All cells have been tested for chromosomal integrity and the absence of pathogens (i.e. mycoplasma, etc). Full characterization included in the Certificate of Analysis.

Restricted Use

For Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures.

Documents

User Guide

• SynFire Users Guide

Application Notes

Long-term functional characterization of SynFire® iNs and astrocytes co-cultured on MaxTwo Multi-Well High-Density Microelectrode Array [Application note]

Application Protocol

SynFire® iN plating protocol and chemical long-term potentiation on MaxWell Biosystems Multi-Well High-Density Microelectrode Array (HD-MEA) [Protocol]

Protocol for MED64 Presto 24-well Sakura MEA plates [Protocol]

Publications using these products 

• Ishibashi Y et al. Responses to antibiotics in human iPSC-derived neurons based on the clinical antibiotic associated encephalopathy classification. J. Toxicol. Sci. (2022).
• Zaer H et al. Non-Ablative Doses of Focal Ionizing Radiation Alters Function of Central Neural Circuits. Brain Stimulation (2022).
• Pediaditakis I et al. A Microengineered Brain-Chip to Model Neuroinflammation in Humans. iSCIENCE (2022).
• Saavedra L et al. Comparison of Acute Effects of Neurotoxic Compounds on Network Activity in Human and Rodent Neural Cultures. Toxicol Sci. (2021). 
• SosciaD et al. A flexible 3-dimensional micro-electrode array for in vitro brain models. Lab Chip (2020).
• Tukker A et al. Towards Animal-Free Neurotoxicity Screening: Applicability of hiPSC-Derived Neuronal Models for In Vitro Seizure Liability Assessment. ALTEX (2020).