SynFire® Glutamatergic Induced Neuron Kit
NC1001-10 / NC1001-20 / NC1001-50 / NC1001-60
Brand: SynFire
Co-culture kits containing glutamatergic induced neurons, astroglia, and all of the media necessary to establish a co-culture model for functional neuron studies. Available in two sizes, small (Cat. No. NC1001-10) and large (Cat. No. NC1001-50). Please see the specifications for kit contents.
| Product name | Product code | Pack size | Price (USD) | Price (GBP) | Price (EUR) |
|---|---|---|---|---|---|
| SynFire® Glutamatergic Induced Neuron Kit | NC1001-10 | 1 kit (small) | $ 1,975.00 | £ 1,762.30 | € 2,058.36 |
| SynFire® Glutamatergic Induced Neuron Kit | NC1001-20 | 1 vial (1.5 million cells) | $ 745.00 | £ 717.21 | € 837.70 |
| SynFire® Glutamatergic Induced Neuron Kit | NC1001-50 | 1 kit (large) | $ 2,950.00 | £ 2,602.46 | € 3,039.67 |
| SynFire® Glutamatergic Induced Neuron Kit | NC1001-60 | 1 vial (7.5 million cells) | $ 1,475.00 | £ 1,307.38 | € 1,527.02 |
Note: prices shown do not include shipping and handling charges.
Product Information
Human SynFire Glutamatergic Induced Neurons co-culture studies
Co-Culture kits contain Glutamatergic, Astroglia, and all of the media necessary to establish a co-culture model for functional neuron studies. Kits containing glutamatergic and GABAergic neurons, astroglia and media Cat. No. NC1010-1.5), or only Glutamatergic neurons, astroglia, and media (Cat. No. NC1002-10) are also available.
Glutamatergic Induced Neuron Kits are available in two sizes, small (Cat. No. NC1001-10) and large (Cat. No. NC1001-50). In addition, the Glutamatergic Induced Neurons are available separately in small (Cat. No. NC1001-20) and large (Cat. No. MC1001-60) sizes. Please see the specification for kit contents.
SynFire iNs (Induced Neurons) are suitable for a variety of biochemical assays. For example, the effect of compounds on neuronal survival, axonal outgrowth, or dendritic arborization can be measured by standard assessment of viability, or image-based analysis of labeled cells, respectively. When co-cultured with glial cells, effects on synapse formation and composition, transcriptional programs, and electrophysiology can be tested. Neuronal subtypes can be mixed in different ratios for making a defined co-culture for different experimental purposes.
Videos: see the NeuCyte Webinar Series online.
NeuCyte company name and logo and SynFire brand name are the property of NeuCyte Inc., USA.
Kit Contents
SynFire Glutamatergic Induced Neuron Kit (Small) (Cat. No. NC1001-10)
- Part No. 1001-1.5: Glutamatergic induced neurons (>1.5x106 cells/vial)
- Part No. 1003-1.5: Astroglia (>1.5x106 cells/vial)
- Part No. 2001-10: Seeding Basal Media (Enough for 10 mL)
- Part No. 2001S-10: Seeding Supplement (Enough for 10 mL)
- Part No. 2002-20: Short-term Basal Media (Enough for 20 mL)
- Part No. 2002S-20: Short-term Supplement (Enough for 20 mL)
- Part No. 2003-60: Long-term Basal Media (Enough for 60 mL)
- Part No. 2003S-60: Long-term Supplement (Enough for 60 mL)
SynFire Glutamatergic Induced Neuron Kit (Large) (Cat. No. NC1001-50)
- Part No. 1001-7.5: Glutamatergic induced neurons (>7.5x106 cells/vial)
- Part No. 1003-3.5: Astroglia (>3.5x106 cells/vial)
- Part No. 2001-20: Seeding Basal Media (Enough for 20 mL)
- Part No. 2001S-20: Seeding Supplement (Enough for 20 mL)
- Part No. 2002-40: Short-term Basal Media (Enough for 40 mL)
- Part No. 2002S-40: Short-term Supplement (Enough for 40 mL)
- Part No. 2003-120: Long-term Basal Media (Enough for 120 mL)
- Part No. 2003S-120: Long-term Supplement (Enough for 120 mL)
SynFire Glutamatergic Induced Neurons(Small) (Cat. No. NC1001-20) Part No. 1002-1.5: GABAergic induced neurons (>1.5x106 cells/vial)
SynFire Glutamatergic Induced Neurons(Large) (Cat. No. NC1001-60) Part No. 1002-7.5: GABAergic induced neurons (>7.5x106cells/vial)
Storage and Stability
Store in cells in liquid nitrogen (Astroglia) or -80°C freezer (iNs) immediately upon receipt. Store other reagents at temperature indicated on the label.
This product is stable for at least 6 months from the date of receiving when stored as directed.
QC
Functional validation of maturation and neural network formation potential of iPSC derived induced neurons has been done on MEAs. All cells have been tested for chromosomal integrity and the absence of pathogens (i.e. mycoplasma, etc). Full characterization included in the Certificate of Analysis.
Restricted Use
For Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures.
User guide:
Application notes:
Application protocols:
- SynFire® iN plating protocol and chemical long-term potentiation on MaxWell Biosystems Multi-Well High-Density Microelectrode Array (HD-MEA) [Protocol]
- Protocol for MED64 Presto 24-well Sakura MEA plates [Protocol]
Videos:
Publications using these products
- Ishibashi Y et al. Responses to antibiotics in human iPSC-derived neurons based on the clinical antibiotic associated encephalopathy classification. J. Toxicol. Sci. (2022).
- Zaer H et al. Non-Ablative Doses of Focal Ionizing Radiation Alters Function of Central Neural Circuits. Brain Stimulation (2022).
- Pediaditakis I et al. A Microengineered Brain-Chip to Model Neuroinflammation in Humans. iSCIENCE (2022).
- Saavedra L et al. Comparison of Acute Effects of Neurotoxic Compounds on Network Activity in Human and Rodent Neural Cultures. Toxicol Sci. (2021).
- SosciaD et al. A flexible 3-dimensional micro-electrode array for in vitro brain models. Lab Chip (2020).
- Tukker A et al. Towards Animal-Free Neurotoxicity Screening: Applicability of hiPSC-Derived Neuronal Models for In Vitro Seizure Liability Assessment. ALTEX (2020).