Additional Media for SynFire Induced Neuron Culture
These media kits provide additional media (over what is included in the neuron kit) to support the culture of GABAergic (Cat. No. NC1002-10), glutamatergic (Cat. No. NC1001-10), and mixed induced neurons (Cat. No. NC1010-1.5).
| Product Name | Catalog number | Summary Description |
| SynFire Complete Media Kit (small) | NC2010-10 | Seeding (10 mL), short-term (20 mL), and long-term (60 mL) media and supplements |
| SynFire Complete Media Kit (large) | NC2010-20 | Seeding (20 mL), short-term (40 mL), and long-term (120 mL) media and supplements |
| SynFire Media (for long term maintenance) | NC2003-1 | Long term media (120 mL) for extended culture |
Custom media configurations are also available: please inquire at info-us@reprocell.com or info-emea@reprocell.com.
Specifications
Kit Contents
SynFire Complete Media Kit (Small) (Cat. No. NC2010-10)
- Part No. 2001-10: Seeding Basal Media (Enough for 10 mL)
- Part No. 2001S-10: Seeding Supplement (Enough for 10 mL)
- Part No. 2002-20: Short-term Basal Media (Enough for 20 mL)
- Part No. 2002S-20: Short-term Supplement (Enough for 20 mL)
- Part No. 2003-60: Long-term Basal Media (Enough for 60 mL)
- Part No. 2003S-60: Long-term Supplement (Enough for 60 mL)
SynFire Complete Media Kit (Large) (Cat. No. NC2010-20)
- Part No. 2001-20: Seeding Basal Media (Enough for 20 mL)
- Part No. 2001S-20: Seeding Supplement (Enough for 20 mL)
- Part No. 2002-40: Short-term Basal Media (Enough for 40 mL)
- Part No. 2002S-40: Short-term Supplement (Enough for 40 mL)
- Part No. 2003-120: Long-term Basal Media (Enough for 120 mL)
- Part No. 2003S-120: Long-term Supplement (Enough for 120 mL)\
SynFire Media (Long Term Maintenance) (Cat. No. NC2003-1)
- Part No. 2003-120: Long-term Basal Media (Enough for 120 mL)
- Part No. 2003S-120: Long-term Supplement (Enough for 120 mL)
Storage and Stability
Store reagents at the temperature indicated on the label.
This product is stable for at least 6 months from the date of receiving when stored as directed.
QC
Full characterization included in the Certificate of Analysis.
Restricted Use
For Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures.
Documents
User Guide
Application Notes
Application Protocols
Protocol for MED64 Presto 24-well Sakura MEA plates [Protocol]
Publications using these products
- Ishibashi Y et al. Responses to antibiotics in human iPSC-derived neurons based on the clinical antibiotic associated encephalopathy classification. J. Toxicol. Sci. (2022).
- Zaer H et al. Non-Ablative Doses of Focal Ionizing Radiation Alters Function of Central Neural Circuits. Brain Stimulation (2022).
- Pediaditakis I et al. A Microengineered Brain-Chip to Model Neuroinflammation in Humans. iSCIENCE (2022).
- Saavedra L et al. Comparison of Acute Effects of Neurotoxic Compounds on Network Activity in Human and Rodent Neural Cultures. Toxicol Sci. (2021).
- SosciaD et al. A flexible 3-dimensional micro-electrode array for in vitro brain models. Lab Chip (2020).
- Tukker A et al. Towards Animal-Free Neurotoxicity Screening: Applicability of hiPSC-Derived Neuronal Models for In Vitro Seizure Liability Assessment. ALTEX (2020).