LDN-193189 is a cell permeable small molecule inhibitor of bone morphogenetic protein (BMP) type I receptors ALK2 and ALK3 (IC50 = 5nM and 30nM respectively)1. LDN-193189 was derived from structure-activity relationship studies of dorsomorphin and functions primarily through prevention of Smad1, Smad5, and Smad8 phosphorylation1-3. LDN-193189 only weakly inhibits ALK4, ALK5, and ALK71. BMP signaling coordinates developmental patterning and has essential physiological roles in mature organisms4,5. LDN-193189has been used to reduce ectopic ossification in a mouse model of fibrodysplasia ossificans progressiva1. Stemolecule LDN-193189 is a hydrochloride salt.
Also available as a 10 mM stock solution (Cat. No. 04-0074-02)
Discontinued Product Cat. No. 04-0019:
Stemolecule LDN-193189 is the suggested replacement for the discontinued product Stemolecule LDN-193189 Free Base (Cat.No. 04-0019). While the free base form was only soluble in chloroform, this salt-based form is soluble in DMSO for your convenience.
Greater than 96% by HPLC analysis
Reconstitute in DMSO to the desired concentration. For reconstitution instructions please reference product specifications sheet.
Storage and Stability
Store powder at 4 °C protected from light. Information about the stability of Stemolecules in solution is largely not available. As a general guideline, we recommend that stock solution be freshly made and stored in aliquots at −20 °C, protected from light. The effect of storage of stock solutions should be verified for each application.
The purity of LDN-193189 was determined by HPLC analysis. The accurate mass was determined by mass spectrometry. No acute cytotoxicity was observed in mouse embryonic stem cells following a 6 hour exposure to 1 nM — 1 µm of LDN-193189.