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Stemolecule™ PD0325901

04-0006 / 04-0006-10 / 04-0006-02

Brand: Stemgent™

PD03225901 inhibits mitogen-activated protein kinase (MAPK/ERK kinase or MEK) and demonstrates potential antineoplastic activity.


Note: prices shown do not include shipping and handling charges.

Product code Pack size The Americas   UK, Europe, Middle East, Africa
(USD)   UK (GBP) Europe (EUR)

04-0006
04-0006-10
04-0006-02

2 mg
10 mg
2 mg in solution (10 mM)

$ 214.00
$ 839.00
$ 251.00

 

£ 165.13
£ 725.92
£ 194.29

€ 187.43
€ 823.94
€ 220.52

  USA store  
The Americas
Product code Pack size USD

04-0006
04-0006-10
04-0006-02

2 mg
10 mg
2 mg in solution (10 mM)

$ 214.00
$ 839.00
$ 251.00

USA store
UK, Europe, Middle East, Africa
Product code Pack size UK (GBP)

04-0006
04-0006-10
04-0006-02

2 mg
10 mg
2 mg in solution (10 mM)

£ 165.13
£ 725.92
£ 194.29

Europe (EUR)

04-0006
04-0006-10
04-0006-02

2 mg
10 mg
2 mg in solution (10 mM)

€ 187.43
€ 823.94
€ 220.52


Stemolecule-04-0006

PD0325901 is a small molecule targeting mitogen-activated protein kinase (MAPK/ERK kinase or MEK) with potential antineoplastic activity. PD0325901, a derivative of MEK inhibitor CI-1040, selectively binds to and inhibits MEK, which may result in the inhibition of the phosphorylation and activation of MAPK/ERK and the inhibition of tumor cell proliferation1,2. Along with the ALK5 inhibitor SB431542, PD0325901 has also been shown to increase the efficiency of reprogramming human primary fibroblasts into induced pluripotent stem (iPS) cells3.

Stemgent conveniently provides all the components comprising the 5i/L/A media supplement necessary to support naive pluripotent stem cell applications.

5i (inhibitors):

L: Stemfactor LIF, Human Recombinant (03-0016), and

A: Stemfactor Activin A, Human Recombinant (03-0001)

Specifications

Product Name: Stemolecule PD0325901

Catalog Number: 04-0006

Size

  • 2 mg (Cat. No. 04-0006)
  • 10 mg (Cat. No. 04-0006-10)
  • 2 mg in 10 mM DMSO (Cat. No. 04-0006-02)

Alternate Name(s): N-[(2R)-2,3-dihydroxypropoxy]-3,4-difluoro-2-[(2-fluoro-4-iodophenyl)amino]-benzamide

Chemical Formula: C16H14F3IN2O4

Molecular Weight: 482.19

CAS Number: 391210-10-9

Purity: Greater than 95 % by HPLC analysis

Formulation: Pale purple solid

Solubility: Reconstitute in DMSO to the desired concentration. For reconstitution instructions please reference product specifications sheet.

Storage and Stability: Store powder at 4 °C protected from light. Information about the stability of Stemolecules in solution is largely not available. As a general guideline, we recommend that stock solution be freshly made and stored in aliquots at −20 °C, protected from light. The effect of storage of stock solutions should be verified for each application.

Quality Control: The purity of PD0325901 was determined by HPLC analysis. The accurate mass was determined by mass spectrometry. No acute cytotoxicity was observed in mouse embryonic stem cells following a 6 hour exposure to 1 nM — 100µm of PD0325901.

Handling: Before opening, briefly centrifuge the vial to ensure full recovery of sample. Aliquotting the stock solution is recommended to avoid repetitive freeze-thaw cycles. For cell culture, the media should be prewarmed prior to adding the reconstituted compound. Note: for most cells, the maximum tolerance to DMSO is less than 0.5%.

References

  1. Bain, J., Plater, L., Elliott, M., Hastie, C.J., McLauchlan, H., Klevernic, I., Arthur, J.S., Alessi, D.R., and Cohen, P. The selectivity of protein kinase inhibitors: a further update. Biochem J 408: 297-315 (2007).
  2. Sebolt-Leopold, J.S. and Herrera, R. Targeting the mitogen-activated protein kinase cascade to treat cancer. Nat Rev Cancer 4: 937-947 (2004).
  3. Lin, T., Ambasudhan, R., Yuan, X., Li, W., Hilcove, S., Abujarour, R., Lin, X., Hahm, H.S., Hao, E., Hayek, A., and Ding, S. A chemical platform for improved induction of human iPSCs. Nat Methods 6: 805-808 (2009).

Additional Publications