XAV939 is an inhibitor of the Wnt / ?-catenin pathway which modulates a number of stem cell behaviors.
|Product name||Product code||Pack size||Price (USD)||Price (GBP)||Price (EUR)|
|Stemolecule™ XAV939||04-0046||2 mg||$ 230.00||£ 214.25||€ 250.24|
Note: prices shown do not include shipping and handling charges.
Stemolecule XAV939 is a cell-permeable small molecule inhibitor of the Wnt / β-catenin pathway. XAV939 inhibits tankyrase 1 and tankyrase 2, thus stabilizing axin and stimulating β-catenin degradation1. Current research suggests that Wnt proteins act to maintain stem cells in an undifferentiated, self-renewing state2. Wnt proteins act on a variety of stem cells that include neural, mammary and embryonic stem cells. In addition, XAV939 inhibits growth of DLD-1 cells, an APC-deficient colorectal cancer cell line1.
Stemgent and the Stemolecule brand name are trademarks of REPROCELL Inc., Japan.
Product Name: Stemolecule XAV939
Catalog Number: 04-0046
Size: 2 mg
Alternate Name(s): 2-(4-(trifluoromethyl)phenyl)-7,8-dihydro-5H-thiopyrano[4,3-d]pyrimidin-4-ol
Chemical Formula: C14H11F3N2OS
Molecular Weight: 312.31
CAS Number: 284028-89-3
Purity: Greater than 95 % by HPLC analysis
Formulation: White powder
Solubility: XAV939 is reported to be soluble in DMSO at 100 mM.
Reconstitution: For a 10 mM concentrated stock solution of XAV939, reconstitute the compound by adding 640.4 µL of DMSO to the entire contents of the vial. If precipitate is observed, warm the solution to 37 °C for 2 to 5 minutes. For use in cell culture, warm the media prior to adding reconstituted compound. Note: for most cells, the maximum tolerance to DMSO is less than 0.5%.
Storage and Stability: Store powder at −20 °C protected from light. Information about the stability of Stemolecules in solution is largely not available. As a general guideline, we recommend that stock solution be freshly made and stored in aliquots at −20 °C, protected from light. The effect of storage of stock solutions should be verified for each application.
Quality Control: The purity of XAV939 is determined by HPLC analysis. The molecular weight is determined by mass spectrometry. No acute cytotoxicity is observed in mouse embryonic stem cells following a 6 hour exposure to 1 nM — 100 uM of XAV939.
Safety Data Sheets:
- Huang, S.M., Mishina, Y.M., Liu, S., Cheung, A., Stegmeier, F., Michaud, G.A., Charlat, O., Wiellette, E., Zhang, Y., Wiessner, S., Hild, M., Shi, X., Wilson, C.J., Mickanin, C., Myer, V., Fazal, A., Tomlinson, R., Serluca, F., Shao, W., Cheng, H., Schultz, M., Rau, C., Schirle, M., Schlegl, J., Ghidelli, S., Fawell, S., Lu, C., Curtis, D., Kirschner, M.W., Lengauer, C., Finan, P.M., Tallarico, J.A., Bouwmeester, T., Porter, J.A., Bauer, A., and Cong, F. Tankyrase inhibition stabilizes axin and antagonizes Wnt signaling. Nature 461: 614-620 (2009)
- Nusse, R. Wnt signaling and stem cell control. Cell Research 18: 523-527 (2008).
- Zhang A; Sokolva I; Domissy A; Davis J; rao L; Utami KH; Wang Y; Hagerman RJ; Pouladi MA; Sanna P; Bolad MH; Loring JF. Maturation Delay of Human GABAergic Neurogenesis in Fragile X Syndrome Pluripotent Stem Cells. Stem Cells Transl Med 11:613 (2022).
- Wang M; Li W; Hao J; Conzales A; Zhao Z; Flores RS; Kuang X; Mu X; Ching T; Tang G; Luo Z; Garciamendez-Mijares EC; Sahoo JK; Wells MF; Niu G; Agrawal P; Quiñonoes-Hinojosa A; Eggan K; Zhang YS. Molecularly cleavable bioinks facilitate high-performance digital light processing-based bioprinting of functional volumetric soft tissues. Nature Commun 13:3317 (2022).
- Li J; Chen : Li D; Lu M; Huang X; Han X; Chen H. Electroacupuncture Promotes the Survival of the Grafted Human MGE Neural Progenitors in Rats with Cerebral Ischemia by Promoting Angiogenesis and Inhibiting Inflammation. Neural Plasticity 2021:4894881 (2021).
- Ressler AK. Reading the Disease Leaves: Signals, signatures and synchrony in neurodevelopmental disorders. Ph. D. Thesis, Columbia University : (2021).
- Susco SG; Arias-Garçia MA; López-Huerta VG; Beccard A; Bara AM; Moffitt J; Korn J; Fu Z; Barrett LE. FMR1 loss in a human stem cell model reveals early changes to intrinsic membrane excitability. Develop Biol 468:93-100 (2020).
- Chu J; Fitzgerald ML; Sehgal M; Manley W; Fitzgerald S; Naung H; Goldberg EM; Anderson SA. Enhanced maturation of human stem cell derived interneurons by mTOR activation . bioRxiv :dx.doi.org/10.1101/777714 (2019).
- Graß T. Modeling familial Alzheimer's disease utilizing gene targeted human induced pluripotent stem cells. Doctoral Thesis, Freie Univerität Berlin : (2018).
- Ooi J; Langley SR; Xu X; Utami KH; Sim B; Huang Y; Harmston NP; Tay YL; Ziaei A; Zeng R; Low D; Aminkeng F; Sobota RM; Ginhoux F; Petretto E; Pouladi MA. Unbiased Profiling of Isogenic Huntington Disease hPSC-Derived CNS and Peripheral Cells Reveals Strong Cell-Type Specificity of CAG Length Effectsty of CAG Length Effects. Cell Reports 26:2494-508.e7 (2019).
- Duong TT; Lim J; Vasireddy V; Papp T; Nguyen H; Leo L; Pan J; Zhou S; Chen HI; Bennet J; Mills JA. Comparative AAV-eGFP Transgene Expression Using Vector Serotypes 1–9, 7m8, and 8b in Human Pluripotent Stem Cells, RPEs, and Human and Rat Cortical Neurons. Stem Cells Int:Article ID: 7281912 (2019).
- Ye P; Chiang YJ; Qi Z; Li Y; Wang S; Liu Y; Li X; Chen Y-G. Tankyrases maintain homeostasis of intestinal epithelium by preventing cell death. PLoS Genetic 14(9):E1007697 (2018).
- Tu J; Cap D; Li L; Cheung H-H; Chan W-Y. MicroRNA profiling during directed differentiation of cortical interneurons from human-induced pluripotent stem cells. FEBS Open Bio 8:502-512 (2018).
- Magadum A; Ding Y; He L; Kim T; Vasudevarao MD; Long Q; Yang K Wickramasinghe N; Renikunta HV; Dubois N; Veidinger G; Yang Q; Engel FB. Live cell screening platform identifies PPARγ as a regulator of cardiomyocyte proliferation and cardiac repair. Cell Res 27:1002 (2017).
- Xu X; Radulescu CI; Utami KH; and Pouladi MA. Obtaining Multi-electrode Array Recordings from Human Induced Pluripotent Stem Cell−Derived Neurons. Bio-protocol 7:e2609 (2017).
- Itokazu T; Hayano Y; Takahashi R; Yamashita T. Involvement of Wnt/β-catenin signaling in the development of neuropathic pain. Neurosci Res 79:34 (2014).
- Voutilainen M; Lindfors PH; Trela EW; Lonnblad D; Shirokova V; Ela T; Rysit R; Schmidt-Ulrich R; Schneider P; Mikkola ML. Ectodysplasin/NF-κB promotes mammary cell fate via Wnt/β-catenin pathway. PLoS Genet 11(11): e1005676. doi:10.1371/journal.pgen.1005676 (2015).