LDN-193189 is a cell permeable small molecule inhibitor of bone morphogenetic protein (BMP) type I receptors ALK2 and ALK3 (IC50 = 5nM and 30nM respectively)1. LDN-193189 was derived from structure-activity relationship studies of dorsomorphin and functions primarily through prevention of Smad1, Smad5, and Smad8 phosphorylation1-3. LDN-193189 only weakly inhibits ALK4, ALK5, and ALK71. BMP signaling coordinates developmental patterning and has essential physiological roles in mature organisms4,5. LDN-193189has been used to reduce ectopic ossification in a mouse model of fibrodysplasia ossificans progressiva1. Stemolecule LDN-193189 is a hydrochloride salt.
Discontinued Product Cat. No. 04-0019: Stemolecule LDN-193189 is the suggested replacement for the discontinued product Stemolecule LDN-193189 Free Base (Cat.No. 04-0019). While the free base form was only soluble in chloroform, this salt-based form is soluble in DMSO for your convenience.
Specifications
Product Name: Stemolecule LDN-193189
Catalog Number: 04-0074
Size:
- 2 mg (Cat. No. 04-0074)
- 10 mg (Cat. No. 04-0074-10)
- 2 mg as a 10 mM Stock Solution in DMSO (Cat. No. 04-0074-02)
Alternate Name(s):
- LDN193189
- 4-(6-(4-(piperazin-1-yl)phenyl)pyrazolo[1,5-a]pyrimidin-3-yl)quinoline hydrochloride
Chemical Formula: C25H22N6·HCl
Molecular Weight: 442.94
CAS Number: 1062368-24-4
Purity: Greater than 96 % by HPLC analysis
Formulation: Yellow powder
Solubility: Reconstitute in DMSO to the desired concentration. For reconstitution instructions please reference product specifications sheet.
Storage and Stability: Store powder at 4 °C protected from light. Information about the stability of Stemolecules in solution is largely not available. As a general guideline, we recommend that stock solution be freshly made and stored in aliquots at −20 °C, protected from light. The effect of storage of stock solutions should be verified for each application.
Quality Control: The purity of LDN-193189 was determined by HPLC analysis. The accurate mass was determined by mass spectrometry. No acute cytotoxicity was observed in mouse embryonic stem cells following a 6 hour exposure to 1 nM — 1 µm of LDN-193189.
Documents
Specification Sheets:
Safety Data Sheets:
References
- Yu, P.B., Deng, D.Y., Lai, C.S., Hong, C.C., Cuny, G.D., Bouxsein, M.L., Hong, D.W., McManus, P.M., Katagiri, T., Sachidanandan, C., Kamiya, N., Fukuda, T., Mishina, Y., Peterson, R.T., and Bloch, K.D. BMP type I receptor inhibition reduces heterotopic [corrected] ossification. Nat Med 14: 1363-1369 (2008).
- Yu, P.B., Hong, C.C., Sachidanandan, C., Babitt, J.L., Deng, D.Y., Hoyng, S.A., Lin, H.Y., Bloch, K.D., and Peterson, R.T. Dorsomorphin inhibits BMP signals required for embryogenesis and iron metabolism. Nat Chem Biol 4: 33-41 (2008).
- Cuny, G.D., Yu, P.B., Laha, J.K., Xing, X., Liu, J.F., Lai, C.S., Deng, D.Y., Sachidanandan, C., Bloch, K.D., and Peterson, R.T. Structure-activity relationship study of bone morphogenetic protein (BMP) signaling inhibitors. Bioorg Med Chem Lett 18: 4388-4392 (2008).
- Heisenberg, C.P., and Solnica-Krezel, L. Back and forth between cell fate specification and movement during vertebrate gastrulation. Curr Opin Genet Dev 18: 311-316 (2008).
- Cain, J.E., Hartwig, S., Bertram, J.F., and Rosenblum, N.D. Bone morphogenetic protein signaling in the developing kidney: present and future. Differentiation 76: 831-842 (2008).
Additional Publications
- Wang M; Li W; Hao J; Conzales A; Zhao Z; Flores RS; Kuang X; Mu X; Ching T; Tang G; Luo Z; Garciamendez-Mijares EC; Sahoo JK; Wells MF; Niu G; Agrawal P; Quiñonoes-Hinojosa A; Eggan K; Zhang YS. Molecularly cleavable bioinks facilitate high-performance digital light processing-based bioprinting of functional volumetric soft tissues. Nature Commun 13:3317 (2022).
- Nie J; Ueda Y; Solivais AJ; Hashino E. CHD7 regulates otic lineage specification and hair cell differentiation in human inner ear organoids. Nature Commun 13:7053 (2022).
- Takao T; Yamada D; Takarda T. A protocol to induce expandable limb-bud mesenchymal cells from human pluripotent stem cells. Star Protocols 3:101786 (2022).
- Cohen B; McPhie D; Ren B; Garcia-Leon J; Verfaille C. SOX10 Expression Levels may be a Critical Mediator of White Matter Deficits in Schizophrenia. BioRivX https://doi.org/10.21203/rs.3.rs-1808873/v1: (2022).
- Gonzàlez BJ; Zhao H; Niu J; Williams DJ; Lee J; Goulbourne CN' Xing Y; Wang Y' Oberholzer J; Blumenkrantz MH; Chen X; LeDuc CA; Chung WK; Colecraft HM; Gromada J; ShenY; Goland RS; Leibel RL; Egli D. Reduced calcium levels and accumulation of abnormal insulin granules in stem cell models of HNF1A deficiency. Commun Biology 5:779 (2022).
- Liang L ;Tian Y; Feng L; Wang C; Feng G; Stacey GN; Shyh-Change N; Wu J; Hu B; Li W: Hao J; Wang L; Wang Y. Single-cell transcriptomics reveals the cell fate transitions of human dopaminergic progenitors derived from hESCs. Stem Cell Res Therap 13:412 (2022).
- Lu V; Doan MT; Roy IJ; Torres A; Teitell MA;. Protocol for germ lineage differentiation of primed human pluripotent stem cells using chemically defined, nutrient-balanced media. Star Protocols 3:101568 (2022).
- Ateaque S. Neurotrophin-3 signalling in neurons derived from human embryonic stem cells. PhD Thesis, Cardiff University : (2022).
- Budjan C; Liu S; Ranga A; Gayen S; Pourquiè. Paraxial mesoderm organoids model development of human somites. eLife 11:e68925 (2022).
- Li Z; Lui KN-C; Lau S-T; Lai FP-L; Li P; Chung P; Wong K; Tam P; Cracia-Barcelo M-M; Hui C-c; Sham P; Ngan E. Hedgehog-induced-oxidative-phosphorylation rescues neuronal differentiation defect of human enteric neural crest cells underlying Hirschsprung disease. Research Square :doi.org/10.21203/rs.3.rs-1433241/v1 (2022).
- Hiller BM; Marmion DJ; Thompson CA; Elliott NA; Eferoff H; Brundin P; Vattis VB; McMahon CW; Kordower JH. Optimizing maturity and dose of iPSC-derived dopamine progenitor cell therapy for Parkinson’s disease. npj Regen Med 7:24 (2022).
- Wen J; Song J; Bai Y; Liu T; Cai X; Mei L ;Ma L ; He C; Feng Y. A Model of Waardenburg Syndrome Using Patient-Derived iPSCs With a SOX10 Mutation Displays Compromised Maturation and Function of the Neural Crest That Involves Inner Ear Development. Front Cell Dev Biol 9:720858 (2022).
- Lu V. The Role of Glutamine in Primed Human Pluripotent Stem Cell Fate. Ph.D. Theses, University of California, Los Angeles : (2022).
- Adachi H; Morizane A; Torikoshi S; Raudzus F; Taniguchi Y; Miyamoto S; Sekiguchi K; Takahashi J. Pretreatment with Perlecan-Conjugated Laminin-E8 Fragment Enhances Maturation of Grafted Dopaminergic Progenitors in Parkinson’s Disease Model. Stem Cell Transl Med 11:767 (2022).
- Khoury-Beaumont N; Prakasam R; Pondugula S; Lagas JS; Matkovich S; Gontarz P' Yang L; Yano H; Kim AH; Rubin JB; Kroll KL. The H3K27M mutation alters stem cell growth, epigenetic regulation, and differentiation potential. BMC Biology 20:124 (2022).
- Habekost M; Qvist P; Denham M; Holm IE; Jørgensen AL. Directly Reprogrammed Neurons Express MAPT and APP Splice Variants Pertinent to Ageing and Neurodegeneration. Molecular Neurobiol 176:1 (2021).
- Sakota, S; Shimokawa F; Funaba M; Murakami M. Isolation of the canine inhibinβB subunit gene and characterization of signalling mediated by canine inhibin βB. Cell Biochem Function 2021:1 (2021).
- Parent AV; Faleo G; Chavez J; Saxton M; Berrios DI; Kerper NR; Tang Q; Hebrok M. Selective deletion of human leukocyte antigens protects stem cell-derived islets from immune rejection. Cell Rep 36:109538 (2021).
- Nakajima T; Nakahata A; Yamada N; Yoshizawa K; Kato TM; Iwassaki M; Zhao C; Kuroki H; Ikeya M. Grafting of iPS cell-derived tenocytes promotes motor function recovery after Achilles tendon rupture. Nature Commun 37:5012 (2021).
- Chapkin RS; Davidson LA; Park H; Jin U-H; Fan Y-Y; Cheng Y; Hensel ME; Landrock KK; Allred C; Menon R; Klemashevich C; Jayaraman A; Safe S. Role of the aryl hydrocarbon teceptor (AhR) in mediating the effects of coffee in the colon. Mol Nutrition Food Res 65:2100539 (2021).
- Peteri U-K; Pitkonen J; de Toma I; Nieminen O; Utami KH; Strandin TM; Corcoran P; Roybon L Vaheri A; Ethell I; Casarotto P; Pouladi MA; Castrén ML. Urokinase plasminogen activator mediates changes in human astrocytes modeling fragile X syndrome. Glia 69:2947 (2021).
- Khalili M; Zarebkohan A; Dianat-Moghadam H; Panahi M; Andre H; Alizadeh E. Corneal endothelial cell sheet bioengineering from neural crest cell-derived adipose stem cells on novel thermo-responsive elastin-mimetic dendrimers decorated with RGD. Chem Engr J 429:132523 (2022).
- Singh T; Jiao Y; Ferrando LM; Yablonska S; Li F; Horoszko EC; Lacomis D; Friedlander RM; Carlisle DL. Neuronal mitochondrial dysfunction in sporadic amyotrophic lateral sclerosis is developmentally regulated. Sci Reports 11:18916 (2021).
- Seo BA; Kim D; Hwang H; Kim MS; Ma S-H; Kweon SH; Wang J; Yoo JM; Choi S; Kwon SH; Kange S-U; Kam T-I; Kim K; Karuppagounder SS; Kang BG; Lee S; Park H; Kim S; Yan W; Li YS; Kuo SH; Redding-Ochoa J; Pletnikova O; Troncosco JC; Lee G' Mao X; Dawson VL; Dawson TM; Ko HS. TRIP12 ubiquitination of glucocerebrosidase contributes to neurodegeneration in Parkinson’s disease. Neuron 23:3753-74.e11 (2021).
- Xi N; Wu J; Ortiz-Vitali JL; Li Y; Darabi R. Directed Differentiation of Human Pluripotent Stem Cells toward Skeletal Myogenic Progenitors and Their Purification Using Surface Markers. Cells 10:2746 (2021).
- Fernandes HJR; Patikas M; Foskolou S; Field SF; Park J-E; Myrne ML; Bassett AR; Metzakopian E. Single-Cell Transcriptomics of Parkinson’s Disease Human In Vitro Models Reveals Dopamine Neuron-Specific Stress Responses. Cell Reports 33:108263 (2020).
- Barbar L; Rusielewica T; Zimmer M; Kalpana K; Fossati V. Isolation of Human CD49f+ Astrocytes and In Vitro iPSC-Based Neurotoxicity Assays. STAR Protocols :doi.org/10.1016/j.xpro.2020.100172 (2020).
- Coyne AN; Zaepfel BL; Hayes L; Fitchman B; Salzberg Y; Luo E-C; Bowen K; Trost H; Aigner S; Rigo F; Yeo GW; Harel A; Csvendsen CN; Sareen D; Rothstein JD. G4C2 Repeat RNA Initiates a POM121-Mediated Reduction in Specific Nucleoporins in C9orf72 ALS/FTD. Neuron 107:1124-1140.e11 (2020).
- Habekost M; Jørgensen AL; Qvist P; Denham M. MicroRNAs and Ascl1 facilitate direct conversion of porcine fibroblasts into induced neurons. Stem Cell Res 48:101984 (2020).
- von Troyer M. Establishment of an alginate based 3D culture system for the generation of dopaminergic neurons. Masters Thesis, Univ Innsbruck : (2020).
- Susco SG; Arias-Garçia MA; López-Huerta VG; Beccard A; Bara AM; Moffitt J; Korn J; Fu Z; Barrett LE. FMR1 loss in a human stem cell model reveals early changes to intrinsic membrane excitability. Develop Biol 468:93-100 (2020).
- Hiller BM; Marmion DJ; Gross RM; Thomson CA; Chavez CA; Brundin P; Wakeman DR; McMahon CW; Kordower JH. Mitomycin-C treatment during differentiation of induced pluripotent stem cell-derived dopamine neurons reduces proliferation without compromising survival or function in vivo. Stem Cells Translational Medicine :DOI: 10.1002/sctm.20-0014 (2020).
- Chen M; Miamaitili M; Habekost M; Gill KP; Mermet-Joret N; Nabavi S; Febbbraro F; Denham M. Rapid generation of regionally specified CNS neurons by sequential patterning and conversion of human induced pluripotent stem cells. Stem Cell Res :https://doi.org/10.1016/j.scr.2020.101945 (2020).
- Chavali VRM; Haider M: Rathi S; Vrathasha V; Alapati T; He J; Gill K; Nikonov R; Duong TT; McDouald DS; Nikonov S; O'Brien J; Mills JA. Dual SMAD inhibition and Wnt inhibition enable efficient and reproducible differentiations of induced pluripotent stem cells into retinal ganglion cells. Sci Rep 10:11828 (2020).
- Mazzara PG; Muggeo S; Luoni M; Massimino L Zaghi M; Valverde PT-T; Brusco S: Marzi MJ; Palma C; Colasante G; Iannielli A; Paulis M; Cordiglieri C;: Giannelli SG; Podini P; Gellera C; Taroni F; Nicassio F; Rasponi M; Broccoli V. Frataxin gene editing rescues Friedreich’s ataxia pathology in dorsal root ganglia organoid-derived sensory neurons. Nature Commun 11:4178 (2020).
- Sook SR; Badell-Grau RA; Kirkham ED; Jones KM; Kelly BP; WInston J; Waller-Evans H; Allen ND; Lloyd-Evans E. Detrimental effect of zwitterionic buffers on lysosomal homeostasis in cell lines and iPSC-derived neurons. AMRC Open Res 2:21 (2020).
- Angelova PR; Choi ML; Berezhnov AV; Horrocks MH; Hughes CD; De S; Redrigues M; Yapom R; Little D; Dolt KS; Kunath T; Devine MJ; Gissen P; Shchepinov MS; Sylantyev S; Pavlov EV; Klenerman D; Abramov AY; Handhi S'. Alpha synuclein aggregation drives ferroptosis: an interplay of iron, calcium and lipid peroxidation. Cell Death Differ :doi.org/10.1038/s41418-020-0542-z (2020).
- Veenvliet JV; Bolondi A; Kretzmer H; Haut L; Scholze-Wittler M; Schifferl D; Koch F; Pustet M; Heimann S; Buschow R; Wittler L; Timmermann B; Meissner A; Herrmann BG. Mouse embryonic stem cells self-organize into trunklike structures with neural tube and somites. bioRxiv :https://doi.org/10.1101/2020.03.04.974949 (2020).
- Ahfeldt T; Ordureau A; Bell C; Sarrafha L; Sun C; Piccinotti S; Crass T; Parfitt GM; Paulo JA; Yanagawa J; Uozumi T; Kiyota Y; Harper JW' Rubin LL. Pathogenic Pathways in Early-Onset Autosomal Recessive Parkinson's Disease Discovered Using Isogenic Human Dopaminergic Neurons. Stem Cell Rep in press:doi.org/10.1016/j.scemcr.2019.12.005 (2020).
- Chu J; Fitzgerald ML; Sehgal M; Manley W; Fitzgerald S; Naung H; Goldberg EM; Anderson SA. Enhanced maturation of human stem cell derived interneurons by mTOR activation . bioRxiv :dx.doi.org/10.1101/777714 (2019).
- Iannielli A; Ugonlini GS; Cordiglieri C; Bido S; Rubio A; Colasante G; Valtorta M; Cabassi T; Rasponi M; Broccoli V. Reconstitution of the Human Nigro-striatal Pathway on-a-Chip Reveals OPA1-Dependent Mitochondrial Defects and Loss of Dopaminergic Synapses. Cell Rep 29:4646-4656.e4 (2019).
- Mahajani S; Raina A; Fokken C; Kügler S; Bähr M. Homogenous generation of dopaminergic neurons from multiple hiPSC lines by transient expression of transcription factors. Cell Death Disease 10:898 (2019).
- Matsumoto R; Suga H; Aoi T; Bando H; Fukuoka H; Iguchi G; Narumi S; Hasegawa T; Muguruma K; Ogawa W; Takahashi Y. Congenital pituitary hypoplasia model demonstrates hypothalamic OTX2 regulation of pituitary progenitor cells. J Clin Investigation in press:doi.org/10.1172/JCI127378 (2019).
- Gantner CWB. Investigating Brain Repair and Development Using Stem Cells. Ph. D. Thesis, University of Melbourne : (2019).
- Huang M; Tailor J; Zhen Q: Gillmor AH; Miller ML; Weishaupt H; Chen J; Zheng T; Nash EK; McHenry LK; An Z; Ye F; Takashima Y; Clarke J; Ayetey H; Cavalli FMG; Luu B; Moriarity BS; Ilkhanizadeh S; Chavez L; Yu C; Kurian KM; Magnaldo T: Sevenet N; Kock P; Pollard SM; Dirks P; Snyder MP; Largaespada DA; Cho YJ; Phillips JJ; Swartling FJ; Morrissy AS; Kool M; Pfister MD; Smith A; Weiss WA; . Engineering Genetic Predisposition in Human Neuroepithelial Stem Cells Recapitulates Medulloblastoma Tumorigenesis. Cell Stem Cell 25:422-446.E7 (2019).
- Koutmani Y; Gampierakis IA; Polissidis A; Ximerakis M: Koutsoudaki PN; Polyzos A; Agrogiannis G; Karaliota S; Thomaidou D; Rubin LL; Politis PK; Karakis KP. CRH Promotes the Neurogenic Activity of Neural Stem Cells in the Adult Hippocampus. Stem Cell Rep 28:932-945.e7 (2019).
- Leitner D; Ramamoorthy M; Dejosez M; Zwaka TP. Immature mDA Neurons Ameliorate Motor Deficits in a 6-OHDA Parkinson's Disease Mouse Model and are Functional after Cryopreservation. Stem Cell Res in press:doi.org/10.1016/j.scr.2019.101617 (2019).
- Nagaraja S; Quezada MA; Gillespie SM; Arzt M; Lennon JJ; Woo PJ; Hovestadt V; Kambhampati M; Filbin MG; Suva ML; Nazarian J; Monje M. Histone Variant and Cell Context Determine H3K27M Reprogramming of the Enhancer Landscape and Oncogenic State. Molecular Cell 76:1-16 (2019).
- Ortiz-Vitali J. iPSC based gene correction and disease model of a new class of LGMD due to POGLUT1 mutation. Master's Thesis Univ of Texas: (2019).
- Li J-J; Lin X; Tang C; Lu Y-Q; Hu X; Zuo E; Li H; Ying W; Sun Y; Lai L-L; Chen H-Z; Guo X0X; Zhang Q-J; Wu S; Zhou C; Shen X; Wang Q: Lin T-T; Ma L-X; Wang N; Krainer AR; Shi L Yang H; Chen W-J. Disruption of splicing-regulatory elements using CRISPR/Cas9 rescues spinal muscular atrophy in human iPSCs and mice. National Sci Rev in press:nwz131 (2019).
- Lee K; Cho H; Rickert RW; Li QV; Pulecia J; Leslie CS; Huangfu D. FOXA2 Is Required for Enhancer Priming during Pancreatic Differentiation. Cell Reports 28:382-393 (2019).
- Lindsay CD; Roth JG; LeSafage BL; Heilshorn SC. Bioprinting of stem cell expansion lattices. Acta Biomaterialia in press:doi.org/10.1016/j.actbio.2019.05.014 (2019).
- Govier-Cole AE; Wood RJ; Fletcher JL; Gonsalvez DF; Merlo D; Cate HS; Murray SS; Xiao J. Inhibiting bone morphogenetic protein 4 type I receptor signaling promotes remyelination by potentiating oligodendrocyte differentiation. eNeuro :ENEURO.0399-18.2019 (2019).
- Freedman DA. The impact of environmental and genetic insults on adult and developing brain oligodendrogenesis. Doctoral Thesis, University of Buffalo : (2019).
- Graß T. Modeling familial Alzheimer's disease utilizing gene targeted human induced pluripotent stem cells. Doctoral Thesis, Freie Univerität Berlin : (2018).
- Harmanto Y; Maki T; Yakagi Y; Miyamoto S; Takahashi J. Xeno‐free culture for generation of forebrain oligodendrocyte precursor cells from human pluripotent stem cells. J Neuro Res 2019:1-18 (2019).
- Qadir MMF; Álvarez-Cubela S; Belle K; Sapir T; Messaggio F; Johnson KB; Umland O; Hardin D; Klein D; Pérez-Álvarez I; Fraker CA; Pastori RL Domínguez-Bendalla J. A Double Fail-Safe Approach to Prevent Tumorigenesis and Select Pancreatic β Cells from Human Embryonic Stem Cells. Stem Cell Rep 3:611-623 (2019).
- Wadhwani AR; Affaneh A; Van Gulden S; Kessler JA. Neuronal Apolipoprotein E4 increases cell death and p‐tau release in Alzheimer's disease. Ann Neurol doi: 10.1002/ana.25455.: (2019).
- Fujisawa S; Komatsubara M; Ogura-Ochi K; Tsukamoto-Yamauchi N; Toma K; Inagaki K; Wada W; Otsuka F; . Orexin A modulates prolactin production by regulating BMP-4 activity in rat pituitary lactotorope cells. Peptides 113:35 (2019).
- Ho WKW; Freem L; Zhao D; Painter KJ; Wolley TE; Gaffney EA; McGrew MJ; Tzika A; Milinkovitch MC; Schneider P; Drusko A; Matthäus F; Glover JD; Wells KL; Johansson JA; Davey MG; Sang HM; Clinton M; Headon DJ. Feather arrays are patterned by interacting signaling and cell density waves. PLOS Biol 17:e3000132 (2019).
- Ooi J; Langley SR; Xu X; Utami KH; Sim B; Huang Y; Harmston NP; Tay YL; Ziaei A; Zeng R; Low D; Aminkeng F; Sobota RM; Ginhoux F; Petretto E; Pouladi MA. Unbiased Profiling of Isogenic Huntington Disease hPSC-Derived CNS and Peripheral Cells Reveals Strong Cell-Type Specificity of CAG Length Effects. Cell Reports 26:2494-508.e7 (2019).
- Wang L; De Solis AJ; Goffer Y; Birkenback KE; Engle SE; Tanis R; Levenson JM; Lu X; Rausch R: Purohit M; Lee J-Y; Tan J; De Rosa MC; DOege CA; Aaron HL; Martins G; Brüning JC; Egli D; Costa R; Berbari N; Leibel RL; Stratigopoulos G. Ciliary gene RPGRIP1L is required for hypothalamic arcuate neuron development. JCI Insight 4:e123337 (2019).
- Chuang C-Y; Yand C-C; Soong B-W; Yu C-C; Chen S-H; Huang H-P; Kuo H-C. Modeling spinocerebellar ataxias 2 and 3 with iPSCs reveals a role for glutamate in disease pathology. Sci Rep 9:1166 (2019).
- van Rhijn, J-R. The role of FOXP2 in striatal circuitry. Ph.D. Thesis, Radboud University Nijmegen : (2019).
- Rajaei B; Massumi M; Wheeler M. Glucose‐Responsiveness of Pancreatic β‐Like (GRP β‐L) Cells Generated from Human Pluripotent Stem Cells. Current Protoclos Human Genetics 100:e71 (2019).
- Duong TT; Lim J; Vasireddy V; Papp T; Nguyen H; Leo L; Pan J; Zhou S; Chen HI; Bennet J; Mills JA. Comparative AAV-eGFP Transgene Expression Using Vector Serotypes 1–9, 7m8, and 8b in Human Pluripotent Stem Cells, RPEs, and Human and Rat Cortical Neurons. Stem Cells Int:Article ID: 7281912 (2019).
- Ma S; Viola R: Sui L; Cherubini V; Barbetti F; Eigli D. β Cell Replacement after Gene Editing of a Neonatal Diabetes-Causing Mutation at the Insulin Locus. Stem Cell Reports https://doi.org/10.1016/j.stemcr.2018.11.006: (2018).
- Wu J; Matthias N; Lo J; Ortiz-Vitali JL; Shieh AW; Wang SH; Darabi R. A Myogenic Double-Reporter Human Pluripotent Stem Cell Line Allows Prospective Isolation of Skeletal Muscle Progenitors. Cell Rep 25:1966-1981 (2018).
- Sun X; Song J; Huang H; Chen H; Qian K. Modeling hallmark pathology using motor neurons derived from the family and sporadic amyotrophic lateral sclerosis patient-specific iPS cells. Stem Cell Res Therapy 9:315 (2018).
- Javed AO; Li Y; Muffat J; Su K-C; Cohen MA; Lungjangwa T; Auborg P; Cheeseman IM; Jaenisch R. Microcephaly Modeling of Kinetochore Mutation Reveals a Brain-Specific Phenotype. Cell Rep 25:368-382.e5 (2018).
- Chen Z;l Ren X; Xu X; Zhang Z; Hui Y; Liu Z; Shi L; Fang Y; Ma L; Liu T; Terheyden-Keighley D; Liu L; Zhang X. Genetic Engineering of Human Embryonic Stem Cells for Precise Cell Fate Tracing during Human Lineage Development. Stem Cell Rep in press:https://doi.org/10.1016/j.stemcr.2018.09.014 (2018).
- Sakai-Takemura F; Narita A; Masuda S; Wakamatsu T; Watanabe N; Nishiyama T; Nogami K; Blanc M; Takeda S; Miyagoe-Suzuki Y. Premyogenic progenitors derived from human pluripotent stem cells expand in floating culture and differentiate into transplantable myogenic progenitors. Sci Reports 8:6555 (2018).
- Tu J; Cap D; Li L; Cheung H-H; Chan W-Y. MicroRNA profiling during directed differentiation of cortical interneurons from human-induced pluripotent stem cells. FEBS Open Bio 8:502-512 (2018).
- Schaefer SA; Higashi A; Loomis B; Schrepfer T; Wan G; Corfas G; Dressler GR; Duncan RK. From Otic Induction to Hair Cell Production: Pax2EGFP Cell Line Illuminates Key Stages of Development in Mouse Inner Ear Organoid Model. Stem Cells Development 27:237-251 (2018).
- Yamashita T; Miyamoto T; Bando T; Ono T; Kobayashi S; Doi A; Araki T; Kato Y; Shirakawa T; Suzuki Y; Yamauchi J; Yoshida S; Sato N. Differentiation of oligodendrocyte progenitor cells from dissociated monolayer and feeder-free cultured pluripotent stem cells. PLoS ONE 12:e171947 (2017).
- Donegan JJ; Tyson JA; Branch SY; Beckstead MJ; Anderson SA; Lodge DJ. Stem cell-derived interneuron transplants as a treatment for schizophrenia: preclinical validation in a rodent model. Mol Psychiatry 22:1942-1501 (2017).
- Xu X; Radulescu CI; Utami KH; and Pouladi MA. Obtaining Multi-electrode Array Recordings from Human Induced Pluripotent Stem Cell−Derived Neurons. Bio-protocol 7:e2609 (2017).
- Sluch VM; Chamling X; Liu MM; Berlinicke CA; Cheng J; Mitchell KL; Welsbie DS; Zack DJ. Enhanced Stem Cell Differentiation and Immunopurification of Genome Engineered Human Retinal Ganglion Cells. Stem Cells Translat Med 6:1972 (2017).
- Lin Y; Linask KL; Mallon B; Johnson K; Kleni M; Beers J; Xie W; Du Y; Liu C; Lai Y; Zou J; Haigney M; Yang H; Rao M; Chen G. Heparin Promotes Cardiac Differentiation of Human Pluripotent Stem Cells in Chemically Defined Albumin-Free Medium, Enabling Consistent Manufacture of Cardiomyocytes. Stem Cells Translat Med 6:527-538 (2017).
- Badenes SM; Fernandes TG; Cordeiro CSM; Boucher S; Kuninger D; Vemuri MC; Diogo MM; Cabral JMS. Defined Essential 8 medium and vitronectin efficiently support xeno-free expansion of human induced pluripotent stem cells in stirred microcarrier culture systems. PLoS ONE 11:e0155296 (2016).
- Carballo-Molina OA; Sánchez-Navarro A; López-Ornelas A; Lara-Rodarte R; Salazar P; Campos-Romo A; Ramos-Meija V; Velasco I. Semaphorin 3C Released from a Biocompatible Hydrogel Guides and Promotes Axonal Growth of Rodent and Human Dopaminergic Neurons. Tissue Eng Part A 22:850-861 (2016).
- Xia N; Zhang P; Fang F; Wang Z; Rothstein M; Agulo B; Chiang R; Taylor J; Reijo Pera RA. Transcriptional comparison of human induced and primary midbrain dopaminergic neurons. Scientific Reports 6:20270. doi:10.1038/srep20270 (2016).
- Koehler KR; Ine J; Longworth-Mills E; Liu X-P; Lee J; Holt JR; Hashino E. Generation of inner ear organoids with functional hair cells from pluripotent stem cells. Nat Biotechnol 35:583-589 (2017).
- Zhu S; Russ HA; Wang X; Zhang M; Ma T; Xu T; Tang S; Hebrok M; Deng S. Human pancreatic β-like cells converted from fibroblasts. Nature Commun 7:10080 (2016).
- Reynolds JC. Efficient in vitro development of photoreceptors from human pluripotent stem cells. MasterÕs Theses and Capstone Projects, Dominican University 169: (2015).
- Doi D; Samata B; Katsukawa M; Kikuchi T; Morizane A; Ono Y; Sekiguchi K; Nakagawa M; Parmar M; Takahashi J. Isolation of Human Induced Pluripotent Stem Cell-Derived Dopaminergic Progenitors by Cell Sorting for Successful Transplantation. Stem Cell Reports 2:337-350 (2014).
- Pollen AA; Nowakowski TJ; Shuga J; Wang X; Leyrat AA; Lui JH; Li Z; Szpankowski L; Fowler B; Chen P; Ramalingam N; Sun G; Thu M; Norris M; Lebofsky R; Toppani D; Kemp DW; Wong M; Clerkson B; Jones BN; Wu S; Knutsson L; Alvaredo B; Wang J; Weaver LS; May AP; Jones RC; Unger MA; Kriegtein AR. Low-coverage single-cell mRNA sequencing reveals cellular heterogeneity and activated signaling pathways in developing cerebral cortex. Nature Biotechnology 32:1053 (2014).
- Nakagawa M; Taniguchi Y; Senda S; Takizawa N; Ichisaka T; Asano K; Morizane A; Doi D; Takahasshi J; Nishizawa M; Yoshida Y; Toyoda T; Osafune K; Sekiguchi K; Yamanaka S. A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells. Scientific Reports 4: 3594 (2014).
- Prè D; Nestor MW; Sproul AA; Jacob S; Koppensteiner P; Chinchalongpom V; Zimmer M; Yamamoto A; Noggle SA; Arancio O. A time course analysis of the electrophysiological properties of neurons differentiated from human induced pluripotent stem cells (iPSCs). PLoS ONE 9(7): e103418 (2014).
- Petros TJ; Maurer CW; Anderson SA; . Enhanced derivation of mouse embryonic stem cell-derived cortical interneurons by induced expression of Nkx2.1. Stem Cell Research 11:647-656 (2013).
- Chiang P-M; Wong PC. Differentiation of an embryonic stem cell to hemogenic endothelium by defined factors: Essential role of bone morphogenetic protein 4. Development 138: 2833-2843 (2011).
- Najm; FJ; Zaremba A; Caprariello AV; Nayak S; Freundt EC; Scacheri PC; Miller RH; Tesar PJ. Rapid and robust generation of functional oligodendrocyte progenitor cells from epiblast stem cells. Nat Methods 8:957-962 (2011).