LDN-193189 is a cell permeable small molecule inhibitor of bone morphogenetic protein (BMP) type I receptors ALK2 and ALK3 (IC50 = 5nM and 30nM respectively)1. LDN-193189 was derived from structure-activity relationship studies of dorsomorphin and functions primarily through prevention of Smad1, Smad5, and Smad8 phosphorylation1-3. LDN-193189 only weakly inhibits ALK4, ALK5, and ALK71. BMP signaling coordinates developmental patterning and has essential physiological roles in mature organisms4,5. LDN-193189has been used to reduce ectopic ossification in a mouse model of fibrodysplasia ossificans progressiva1. Stemolecule LDN-193189 is a hydrochloride salt.
Discontinued Product Cat. No. 04-0019: Stemolecule LDN-193189 is the suggested replacement for the discontinued product Stemolecule LDN-193189 Free Base (Cat.No. 04-0019). While the free base form was only soluble in chloroform, this salt-based form is soluble in DMSO for your convenience.
Specifications
Product Name: Stemolecule LDN-193189
Catalog Number: 04-0074
Size:
- 2 mg (Cat. No. 04-0074)
- 10 mg (Cat. No. 04-0074-10)
- 2 mg as a 10 mM Stock Solution in DMSO (Cat. No. 04-0074-02)
Alternate Name(s):
- LDN193189
- 4-(6-(4-(piperazin-1-yl)phenyl)pyrazolo[1,5-a]pyrimidin-3-yl)quinoline hydrochloride
Chemical Formula: C25H22N6·HCl
Molecular Weight: 442.94
CAS Number: 1062368-24-4
Purity: Greater than 96 % by HPLC analysis
Formulation: Yellow powder
Solubility: Reconstitute in DMSO to the desired concentration. For reconstitution instructions please reference product specifications sheet.
Storage and Stability: Store powder at 4 °C protected from light. Information about the stability of Stemolecules in solution is largely not available. As a general guideline, we recommend that stock solution be freshly made and stored in aliquots at −20 °C, protected from light. The effect of storage of stock solutions should be verified for each application.
Quality Control: The purity of LDN-193189 was determined by HPLC analysis. The accurate mass was determined by mass spectrometry. No acute cytotoxicity was observed in mouse embryonic stem cells following a 6 hour exposure to 1 nM — 1 µm of LDN-193189.
References
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