Stemolecule™ LDN-193189

LDN-193189 is a cell permeable small molecule inhibitor of bone morphogenetic protein (BMP) type I receptors ALK2 and ALK3 (IC₅₀ = 5nM and 30nM respectively)¹. LDN-193189 was derived from structure-activity relationship studies of dorsomorphin and functions primarily through prevention of Smad1, Smad5, and Smad8 phosphorylation^1-3. LDN-193189 only weakly inhibits ALK4, ALK5, and ALK7¹. BMP signaling coordinates developmental patterning and has essential physiological roles in mature organisms^4,5. LDN-193189has been used to reduce ectopic ossification in a mouse model of fibrodysplasia ossificans progressiva¹. Stemolecule LDN-193189 is a hydrochloride salt.

Stemolecule LDN-193189 is also available as a 10 mM DMSO Stock Solution in DMSO  (2 mg in 451.5 μl) (Cat. No. 04-0074-02).

Discontinued Product Cat. No. 04-0019: Stemolecule LDN-193189 is the suggested replacement for the discontinued product Stemolecule LDN-193189 Free Base (Cat.No. 04-0019). While the free base form was only soluble in chloroform, this salt-based form is soluble in DMSO for your convenience.

Stemgent and the Stemolecule brand name are trademarks of REPROCELL Inc., Japan.

Product Name: Stemolecule LDN-193189

Catalog Number: 04-0074

Size:

• 2 mg (Cat. No. 04-0074)
• 10 mg (Cat. No. 04-0074-10)
• 2 mg as a 10 mM Stock Solution in DMSO (2 mg in 451.5 μl) (Cat. No. 04-0074-02)

Alternate Name(s):

• LDN193189
• 4-(6-(4-(piperazin-1-yl)phenyl)pyrazolo[1,5-a]pyrimidin-3-yl)quinoline hydrochloride

Chemical Formula: C₂₅H₂₂N₆·HCl

Molecular Weight: 442.94 (check CoA for exact formula weight including solvent of crystallization)

CAS Number: 1062368-24-4

Purity: Greater than 96 % by HPLC analysis

Formulation: Yellow to Orange solid or powder

Solubility: Reconstitute in DMSO to the desired concentration.

Storage and Stability: Store powder at 4 °C protected from light. Information about the stability of Stemolecules in solution is largely not available. As a general guideline, we recommend that stock solution be freshly made and stored in aliquots at −20 °C, protected from light. The effect of storage of stock solutions should be verified for each application.

Quality Control: The purity of LDN-193189 was determined by HPLC analysis. The accurate mass was determined by mass spectrometry. No acute cytotoxicity was observed in mouse embryonic stem cells following a 6 hour exposure to 1 nM — 1 µm of LDN-193189.

Specification Sheets:

• 04-0074 Specifications Sheet
• 04-0074-10 Specifications Sheet
• 04-0074-02 Specifications Sheet

Safety Data Sheets:

• 04-0074 and 04-0074-10 Safety Data Sheet
• 04-0074-02 Safety Data Sheet