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Stemolecule™ Wnt Inhibitor IWP-2

04-0034

Brand: Stemgent™

Wnt Inhibitor IWP-2 prevents palmitylation of Wnt proteins by Porcupine (Porcn), a membrane-bound O-acyltransferase, thereby blocking Wnt secretion and activity. It also blocks phosphorylation of the Lrp6 receptor and accumulation of both Dvl2 and β-catenin.


Note: prices shown do not include shipping and handling charges.

Product code Pack size The Americas   UK, Europe, Middle East, Africa
(USD)   UK (GBP) Europe (EUR)

04-0034

2 mg

$ 114.00

 

£ 93.44

€ 109.14

  USA store  
The Americas
Product code Pack size USD

04-0034

2 mg

$ 114.00

USA store
UK, Europe, Middle East, Africa
Product code Pack size UK (GBP)

04-0034

2 mg

£ 93.44

Europe (EUR)

04-0034

2 mg

€ 109.14


Stemolecule-04-0034

Stemolecule Wnt Inhibitor IWP-2 was identified in a high throughput screen for antagonists of the Wnt/β-catenin pathway. Wnt Inhibitor IWP-2 prevents palmitylation of Wnt proteins by Porcupine (Porcn), a membrane-bound O-acyltransferase, thereby blocking Wnt secretion and activity. It also blocks phosphorylation of the Lrp6 receptor and accumulation of both Dvl2 and β-catenin1.

Specifications

Product Name: Stemolecule Wnt Inhibitor IWP-2

Catalog Number: 04-0034

Size: 2 mg

Alternate Name(s): N-(6-Methyl-2-benzothiazolyl)-2-[(3,4,6,7-tetrahydro-4-oxo-3-phenylthieno[3,2 d]pyrimidin-2-yl)thio]-acetamide

Chemical Formula: C22H18N4O2S3

Molecular Weight: 466.6

CAS Number: 686770-61-6

Purity: Greater than 99 % by HPLC analysis

Formulation: White powder

Solubility: This molecule is reported to be soluble in DMSO at 5 mM.

Reconstitution: For a 5 mM concentrated stock solution of Wnt Inhibitor IWP-2, reconstitute the compound by adding 857.3 µL of DMSO to the entire contents of the vial. If precipitate is observed, warm the solution to 37°C for 2 to 5 minutes.  For cell culture, the media should be prewarmed prior to adding the reconstituted compound. Note: for most cells, the maximum tolerance to DMSO is less than 0.5%. 

Storage and Stability: Store powder at 4 °C protected from light. Information about the stability of Stemolecules in solution is largely not available. As a general guideline, we recommend that stock solution be freshly made and stored in aliquots at −20 °C, protected from light. The effect of storage of stock solutions should be verified for each application.

Quality Control: The purity of Wnt Inhibitor IWP-2 was determined by HPLC analysis. The accurate mass was determined by mass spectrometry. Cellular toxicity of Wnt Inhibitor IWP-2 was tested on mouse embryonic stem cells.

Documents

Specification Sheets:

Safety Data Sheets:

References

  1. Chen, B., Dodge, M.E., Tang, W., Lu, J., Ma, Z., Fan, C.W., Wei, S., Hao, W., Kilgore, J., Williams, N.S., Roth, M.G., Amatruda, J.F., Chen, C., and Lum, L. Small molecule-mediated disruption of Wnt-dependent signaling in tissue regeneration and cancer. Nat Chem Biol 5: 100-107 (2009).

Additional Publications