Stemolecule™ Wnt Inhibitor IWP-2

Stemolecule Wnt Inhibitor IWP-2 was identified in a high throughput screen for antagonists of the Wnt/β-catenin pathway. Wnt Inhibitor IWP-2 prevents palmitylation of Wnt proteins by Porcupine (Porcn), a membrane-bound O-acyltransferase, thereby blocking Wnt secretion and activity. It also blocks phosphorylation of the Lrp6 receptor and accumulation of both Dvl2 and β-catenin¹.

Specifications

Product Name: Stemolecule Wnt Inhibitor IWP-2

Catalog Number: 04-0034

Size: 2 mg

Alternate Name(s): N-(6-Methyl-2-benzothiazolyl)-2-[(3,4,6,7-tetrahydro-4-oxo-3-phenylthieno[3,2 d]pyrimidin-2-yl)thio]-acetamide

Chemical Formula: C₂₂H₁₈N₄O₂S₃

Molecular Weight: 466.6

CAS Number: 686770-61-6

Purity: Greater than 99 % by HPLC analysis

Formulation: White powder

Solubility: This molecule is reported to be soluble in DMSO at 5 mM.

Reconstitution: For a 5 mM concentrated stock solution of Wnt Inhibitor IWP-2, reconstitute the compound by adding 857.3 µL of DMSO to the entire contents of the vial. If precipitate is observed, warm the solution to 37°C for 2 to 5 minutes.  For cell culture, the media should be prewarmed prior to adding the reconstituted compound. Note: for most cells, the maximum tolerance to DMSO is less than 0.5%. 

Storage and Stability: Store powder at 4 °C protected from light. Information about the stability of Stemolecules in solution is largely not available. As a general guideline, we recommend that stock solution be freshly made and stored in aliquots at −20 °C, protected from light. The effect of storage of stock solutions should be verified for each application.

Quality Control: The purity of Wnt Inhibitor IWP-2 was determined by HPLC analysis. The accurate mass was determined by mass spectrometry. Cellular toxicity of Wnt Inhibitor IWP-2 was tested on mouse embryonic stem cells.

Documents

Specification Sheets:

• 04-0034 Specifications Sheet

Safety Data Sheets:

• 04-0034 Safety Data Sheet

References

1. Chen, B., Dodge, M.E., Tang, W., Lu, J., Ma, Z., Fan, C.W., Wei, S., Hao, W., Kilgore, J., Williams, N.S., Roth, M.G., Amatruda, J.F., Chen, C., and Lum, L. Small molecule-mediated disruption of Wnt-dependent signaling in tissue regeneration and cancer. Nat Chem Biol 5: 100-107 (2009).

Additional Publications

• Choi JH; Jun JH; Kim GD; Jho E-h; Baik SK; Bae SH; Kim GJ. Wnt Pathway Activation by Placental Mesenchymal Stem Cells Promotes Hepatic Regeneration in a Cirrhotic Rat Model. Research Square :https://doi.org/10.21203/rs.3.rs-850530/v1 (2021).
• Lovatt M; Yam GH-F; Peh GS; COlman A; Dunn NR; Mehta JS. Directed differentiation of periocular mesenchyme from human embryonic stem cells. Differentiation 99:https://doi.org/10.3892/ol.2018.9292 (2018)
• Gartz M; Lin C-W; Sussman MA; Lawlor MW; Strande JL. Duchenne muscular dystrophy (DMD) cardiomyocyte-secreted exosomes promote the pathogenesis of DMD-associated cardiomyopathy. Diseaase Models Mechanisms 13:dmm045559 (2020).
• Narcisi R; Koevoet WJLM; van Osch GJVM. Expansion and Chondrogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stromal Cells. Osteoporosis Osteoarthritis 2221:15-28 (2020).
• Böttcher M. Single-cell transcriptome analyses on developmental transitions in mouse pluripotent stem cells. Ph. D. Thesis, Univ Bielefeld : (2020).
• Lõhmussaar K; Kopper O; Korving J; Begthel H; Vreuls CPH; van Es JH; Clevers H.. Assessing the origin of high-grade serous ovarian cancer using CRISPR-modification of mouse organoids. Nature Commun 11:2660 (2020).
• Gartz M; Darlington A; Afzal MZ; Strande JL. Exosomes exert cardioprotection in dystrophin-deficient cardiomyocytes via ERK1/2-p38/MAPK signaling. Sci Rep 8:16519 (2018).
• Kondo M; Sugimoto M; Abe K. A Simplified and Efficient Protocol for Derivation and Maintenance of High‐Quality Mouse Primed Pluripotent Stem Cells Using Wnt Inhibition. Curr Protocols Stem Cell Biol 46:e60 (2018).
• Chhabra S; Liu L; Goh R; WarmflashA. The timing of signaling events in the BMP, WNT, and Nodal cascade determines self-organized fate patterning in human gastruloids. bioRxiv 2018:http://dx.doi.org/10.1101/440164 (2018).
• Matsumoto N; Aatyam A; Geha M; Lapchak PH; Dalle Lucca JJ; Tsokos MG; Tsokos GC. C3a Enhances the Formation of Intestinal Organoids through C3aR1 . Fronteirs in Immunology 8:1149-1160 (2017)
• Cho SW; Park J-S; Heo HJ; Park S-W; Song S; Kim I; Han Y-M; Yanashita JK; Youm JB; Han J; Koh GY. Dual modification of the mitochondrial permeability transition pore and redox signaling synergistically promotes cardiomyocyte differentiation from pluripotent stem cells. J Am Heart Assoc 2014;3:e000693 (2014).
• Tan H-K; Toh C-XDl Ma D; Yang B; Liu TM; Lu J; Wong C-W; Tan T-K; Li H; SYn C; Tan E-L; Lim B; Lim Y-P; Cook SA; Loh Y-H. Human finger-prick induced pluripotent stem cells facilitate the development of stem cell banking. Stem Cells Trans Medicine 3:586 (2014).
• Blauwkamp TA; Nigam S; Ardehali R; Weissman IL; Nusse R. Endogenous Wnt signalling in human embryonic stem cells generates an equilibrium of distinct lineage-specified progenitors. Nature Communications 3:1070 (2012).