Stemolecule™ hES Cell Cloning & Recovery Supplement

The hES Cell Cloning and Recovery Supplement is a 1000× concentrate supplement that contains thiazovivin at 2 mM concentration. The Supplement can be used in several areas of cell culture to enhance productivity and efficiency. The addition of the hES Cell Cloning and Recovery Supplement to cell culture media significantly improves the likelihood of successful sub-cloning of pluripotent stem cells (PSCs) from single cells^1,2. This Supplement was validated at a working concentration of 2 μM; however optimal conditions can vary, and they should be determined by the user. When added during a thaw, this Supplement boosts recovery and thawing efficiency. The use of this Supplement also increases attachment after passaging. The hES Cell Cloning and Recovery Supplement is a valuable tool for improving efficiency when working with human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) under stressful conditions.

Stemgent and the Stemolecule brand name are trademarks of REPROCELL Inc., Japan.

Product Name: hES Cell Cloning and Recovery Supplement

Catalog Number: 01-0014-500

Size: 5 × 100 µL

Storage and Stability: Store vials at 4 °C. Supplement is stable for a minimum of 3 months when stored as directed.

Quality Control: Human Pluripotent Stem (hPS) cells were trypsinized and plated with or without the hES Cell Cloning and Recovery Supplement. hES cells cultured with the hES Cell Cloning and Recovery Supplement were shown to have more AP positive colonies than cells cultured without the supplement.

Notice To Purchaser: Purchaser represents and warrants that it will use hES Cell Cloning and Recovery Supplement purely for research purposes, not for diagnostic use, not for resale, and not for use in humans or veterinary applications. Stemgent will not be held responsible for patent infringement or other violations that may occur with the use of our products.

Specification Sheets:

• 01-0014-500 Specifications Sheet

Safety Data Sheets:

• 01-0014-500 Safety Data Sheet

Protocols:

• Protocol: hES Cell Cloning and Recovery Supplement

1. Lin, T., Ambasudhan, R., Yuan, X., Li, W., Hilcove, S., Abujarour, R., Lin, X., Hahm, H.S., Hao, E., Hayek, A., and Ding S. A chemical platform for improved induction of human iPSCs. Nature Methods 6:805 (2009).
2. Xu, Y., Zhu, X., Hahm, H.S., Wei, W., Hao, E., Hayek, A., and Ding, S. Revealing a core signaling regulatory mechanism for pluripotent stem cell survival and self-renewal by small molecules. Proc Natl Acad Sci USA 107: 8129-8134 (2010).

Additional publications

• Verstegen NNAl Roos FJM; Burka K; Gehartg J; Jager M; de Wolf M; Bijvelds MJC; de Jonge HR; Ardisasmita AI; van Huizen NA; Roest HP; de Jonge J; Koch M; Pampaloni F; Fucha SA; Schene IF; Luider TM; van der Doef HPJ; Bodewes FAJA; de Kleine RHJ; Spee B; Kremers G-J; Clevers HJ; IJzermans JMN; Cuppen E; van der Laan LJW. Human extrahepatic and intrahepatic cholangiocyte organoids show region-specific differentiation potential and model cystic fibrosis-related bile duct disease. Sci Rep 10:21900 (2020).
• Hasaart Karlijn AL; Manders F; van der Hoorn M-L; Verheul M; Poplonski T; Kuijk E; Chuva de Sousa Lopes SM; van Boxtel R. Mutation accumulation and developmental lineages in normal and Down syndrome human fetal haematopoiesis. Sci Reports 10:12991 (2020).
• Sivapatham R; Zeng X.. Generation and characterization of patient-specific induced pluripotent stem cell for disease modeling. Methods Mol Biol 1353:25-44 (2014)