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Stemolecule™ hES Cell Cloning & Recovery Supplement

01-0014-500

Brand: Stemgent™

Stemgent hES Cell Cloning & Recover Supplement is a 1000× concentrate of Thiazovivin (2mM). In culture media it significantly improves the likelihood of primaryful sub-cloning from single cells, and increases attachment after passaging. This supplement is a ROCK inhibitor (see Thiazovivin, cat. # 04-0017 for more information).


Note: prices shown do not include shipping and handling charges.

Product code Pack size The Americas   UK, Europe, Middle East, Africa
(USD)   UK (GBP) Europe (EUR)

01-0014-500

5 × 100 μL

$ 161.00

 

£ 124.38

141.17

  USA store  
The Americas
Product code Pack size USD

01-0014-500

5 × 100 μL

$ 161.00

USA store
UK, Europe, Middle East, Africa
Product code Pack size UK (GBP)

01-0014-500

5 × 100 μL

£ 124.38

Europe (EUR)

01-0014-500

5 × 100 μL

141.17


The hES Cell Cloning and Recovery Supplement is a 1000X concentrate supplement that contains Thiazovivin at 2mM concentration. The hES Cell Cloning and Recovery Supplement can be used in several areas of cell culture to enhance productivity and efficiency. The addition of the hES Cell Cloning and Recovery Supplement to cell culture media significantly improves the likelihood of successful sub-cloning from single cells1,2. This supplement was validated at a working concentration of 2 uM, however optimal conditions can vary and should be determined by the user.When added during a thaw, this supplement boosts recovery and thawing efficiency. The use of the hES Cell Cloning and Recovery Supplement also increases attachment after passaging. The hES Cell Cloning and Recovery Supplement is a valuable tool for escalating efficiency when working with human embryonic stem (hES) and human induced pluripotent stem (hiPS) cells under stressful conditions.

Specifications

Product Name: hES Cell Cloning and Recovery Supplement

Catalog Number: 01-0014-500

Size: 5 × 100 µL

Storage and Stability: Store vials at 4 °C. Supplement is stable for a minimum of 3 months when stored as directed.

Quality Control: Human Pluripotent Stem (hPS) cells were trypsinized and plated with or without the hES Cell Cloning and Recovery Supplement. hES cells cultured with the hES Cell Cloning and Recovery Supplement were shown to have more AP positive colonies than cells cultured without the supplement.

Notice To Purchaser: Purchaser represents and warrants that it will use hES Cell Cloning and Recovery Supplement purely for research purposes, not for diagnostic use, not for resale, and not for use in humans or veterinary applications. Stemgent will not be held responsible for patent infringement or other violations that may occur with the use of our products.

Documents

Specification Sheets:

Safety Data Sheets:

Protocols:

References

  1. Lin, T., Ambasudhan, R., Yuan, X., Li, W., Hilcove, S., Abujarour, R., Lin, X., Hahm, H.S., Hao, E., Hayek, A., and Ding S. A chemical platform for improved induction of human iPSCs. Nature Methods 6:805 (2009).
  2. Xu, Y., Zhu, X., Hahm, H.S., Wei, W., Hao, E., Hayek, A., and Ding, S. Revealing a core signaling regulatory mechanism for pluripotent stem cell survival and self-renewal by small molecules. Proc Natl Acad Sci USA 107: 8129-8134 (2010).

Additional publications