Stemolecule™ Thiazovivin

Thiazovivin is a selective, cell permeable small molecule that directly targets Rho-associated kinase (ROCK)¹. In addition, Thiazovivin protects human embryonic stem cells (hESCs) in the absence of ECM by regulating E-cadherin mediated cell-cell interaction¹. This observation suggests that Thiazovivin promotes cell survival. In other studies Thiazovivin, in combination with inhibitors of the TGFβ receptor and MEK pathway, has shown to improve reprogramming efficiency by more than 200-fold².

Stemgent and the Stemolecule brand name are trademarks of REPROCELL Inc., Japan.

Product Name: Stemolecule Thiazovivin

Catalog Number: 04-0017

Size: 1 mg

Chemical Name: N-benzyl-2-(pyrimidin-4-ylamino)thiazole-4-carboxamide

Chemical Formula: C₁₅H₁₃N₅OS

Molecular Weight: 311.36

CAS Number: 1226056-71-8

Purity: Greater than 98 % by HPLC analysis

Formulation: Light brown powder

Solubility: Thiazovivin is soluble in DMSO at 100 mM.

Reconstitution: For a 10 mM concentrated stock solution of Thiazovivin, reconstitute the compound by adding 321.2 µL of DMSO to the entire contents of the vial. If precipitate is observed, warm the solution to 37 °C for 2 to 5 minutes. For use in cell culture, warm the media prior to adding thiazovivin.  As a general rule, cells in culture are sensitive to DMSO in the medium at 0.5% or less.

Storage and Stability: Store powder at 4 °C protected from light. Information about the stability of Stemolecules in solution is largely not available. As a general guideline, we recommend that stock solution be freshly made and stored in aliquots at −20 °C, protected from light. The effect of storage of stock solutions should be verified for each application.

Quality Control: The purity of Thiazovivin was determined by HPLC analysis. The accurate mass was determined by mass spectrometry. No acute cytotoxicity was observed in mouse embryonic stem cells following a 6 hour exposure to 1 nM — 100 µM of Thiazovivin.

Specification Sheets:

• 04-0017 Specifications Sheet

Safety Data Sheets:

• 04-0017 Safety Data Sheet

1. Xu, Y., Zhu, X., Hahm, H.S., Wei, W., Hao, E., Hayek, A., and Ding, S. Revealing a core signaling regulatory mechanism for pluripotent stem cell survival and self-renewal by small molecules. Proc Natl Acad Sci USA 107: 8129-8134 (2010).
2. Lin, T., Ambasudhan, R., Yuan, X., Li, W., Hilcove, S., Abujarour, R., Lin, X., Hahm, H.S., Hao, E., Hayek, A., and Ding, S. A chemical platform for improved induction of human iPSCs. Nat Methods 6: 805-808 (2009).

Additional Publications

• Hageb A; Thalheim T: Nattamai KJ; Möhrle B; Saçma M; Sakk V; Thielecke L; Cornils K; Grandy C; Port F; Gottschalk K-E; Mallm J-P; Glauche I; Galle J; Mulaw MA; Geiger H. Reduced adhesion of aged intestinal stem cells contributes to an accelerated clonal drift. Life Science Alliance 5:DOI: 10.26508/lsa.202201408 (2022).
• He X; Smith SE; Chen S; Lil H; Wu D; Meneses-Giles PI; Wang Y; Hembree M; Yi K; Zhao X; Guo F; Unruh JR; Maddera LE; Ye Z; Scott A; Perera A; Wang Y; Zhao C; Bae KM; Box A; Haug JS; Tao F; Hu D; Hansen DM; Qian P; Saha S; Dixon D; Anant S; Zhang D; Lin EH; Sun W; Wiedemann LM; Li L;. Tumor-initiating stem cell shapes its microenvironment into an immunosuppressive barrier and pro-tumorigenic niche. Cell Rep 36:109674 (2021).
• Yang F; Pham T-A; Brandenberg N; Lutolf MP; Ma J; Unser M. Robust phase unwrapping via deep image prior for quantitative phase imaging. arxiv :2009.11554.pdf (2020).
• Paterson YZ; Cribbs A; Espenel M; Smith EJ; Henson FMD; Guest DF. Genome-wide transcriptome analysis reveals equine embryonic stem cell-derived tenocytes resemble fetal, not adult tenocytes. Stem Cell Res Therap 11:184 (2020).
• Long P; Liu Z; Wu B; Chen J; Sun C; Wang F; Huang Y; Chen H; Li Q; Ma Y. Generation of an induced pluripotent stem cell line from chorionic villi of a Patau syndrome spontaneous abortion. Stem Cell Res 45:101789 (2020).
• Durens M; Nestor J; Herold K Niescier RF; Lunden FW; Phillips AW; Lin Y-C; Nestor MW. High-content interrogation of human induced pluripotent stem cell-derived cortical organoid platforms. bioRxiv http://dx.doi.org/10.1101/697623. : (2019).
• McClellan A; Evans R; Sze C; Kan S; Paterson Y; Guest D. A novel mechanism for the protection of embryonic stem cell derived tenocytes from inflammatory cytokine interleukin 1 beta. Sci Rep 9:2755 (2019).
• Duong TT; Lim J; Vasireddy V; Papp T; Nguyen H; Leo L; Pan J; Zhou S; Chen HI; Bennet J; Mills JA. Comparative AAV-eGFP Transgene Expression Using Vector Serotypes 1–9, 7m8, and 8b in Human Pluripotent Stem Cells, RPEs, and Human and Rat Cortical Neurons. Stem Cells Int:Article ID: 7281912 (2019).
• Kilander MB; Wang C-H; Chang C-H; Nestor JE; Herold K; Tsai J-W; Nestor MW; Lin Y-C. A rare human CEP290 variant disrupts the molecular integrity of the primary cilium and impairs Sonic Hedgehog machinery. Sci Rep 8:17335 (2018).
• Zhu S; Russ HA; Wang X; Zhang M; Ma T; Xu T; Tang S; Hebrok M; Deng S. Human pancreatic β-like cells converted from fibroblasts. Nature Commun 7:10080 (2016).
• Zimmerlan L; Park TS; Huo JS; Verma K; Pather SR; Talbot CC; Agarwal J; Steppan D; Zhang YW; Considine M; Guo H; Zhong X; Gutierrez C; Cope L; Canto-Soler MV; Friedman AD; Baylin SB; Zambdis ET. Tankyrase inhibition promotes a stable human naive pluripotent state with improved functionality. Development 2016:dev.138982 (2016).
• Paull D; Sevilla A; Zhou H; Hahn AK; Kim H; Napolitano C; Tsankov A; Shang L; Krumholz K; Jagaseesan P; Woodard CM; Sun B; Vilboux T; Zimmer M; Forero E; Moroziewicz DN; Martinez H; Malicdan MCV; Weiss KA; Vensand LB; Dusenberry CR; Polus H; Sy KTL; Kahler DJ; Gahl WA; Solomon SL; Chang S; Meissner A; Eggan K; Noggle SA. Automated, high-throughput derivation, characterization and differentiation of induced pluripotent stem cells. Nature Methods
• DeRosa BA; Belle KC; Thomas BJ; Cukier HN; Pericak-Vance MA; Vance JM; Dykxhoom DM. hVGAT-mCherry: A novel molecular tool for analysis of GABAergic neurons derived from human pluripotent stem cells. Mol Cell Neurosci 68:244 (2015).
• Poleganov MA; Eminli S; Beissert T; Herz S; Moon J-I; Goldmann J; Beyer A; Heck R; Burkhart I; Roldan DB; Tureci O; Yi K; Hamilton B; Sahin U. Efficient reprogramming of human fibroblasts and blood-derived endothelial progenitor cells using nonmodified RNA for reprogramming and immune evasion. Human Gene Therapy 26:751 (2015).
• Kahler DJ; Ahmad AS; Ritz A; Hua H; Moroziewicz DN; Sproul AA; Dusenberry CR; Shang L; Paull D; Zimmer M; Weiss KA; Egli D; Noggle SA. Improved Methods for Reprogramming Human Dermal Fibroblasts Using Fluorescence Activated Cell Sorting.. PLoS One 8(3):e59867 (2013).
• Nagy, K., Sung, H-K., Zhang, P., Laflamme, S., Vincent, P, Agha-Mohammadi, S., Woltjen, K., Monetti, C., Michael, I. P., Smith, L. C., Nagy, A. Induced Pluripotent Stem Cell Lines Derive from Equine Fibroblasts. Stem Cell Reviews and Reports 7:693 (2011).