Stemolecule™ Dorsomorphin
04-0024
Brand: Stemgent™
Dorsomorphin dihydrochloride (a.k.a. Compound C) is a potent inhibitor of AMP-activated protein kinase (AMPK) and bone morphogenic protein (BMP) signaling.
Note: prices shown do not include shipping and handling charges.
Product code | Pack size | The Americas | UK, Europe, Middle East, Africa | ||
---|---|---|---|---|---|
(USD) | UK (GBP) | Europe (EUR) | |||
04-0024 |
2 mg |
$ 132.00 |
£ 108.20 |
€ 126.37 |
|
USA store |
The Americas | ||
---|---|---|
Product code | Pack size | USD |
04-0024 |
2 mg |
$ 132.00 |
USA store |
UK, Europe, Middle East, Africa | ||
---|---|---|
Product code | Pack size | UK (GBP) |
04-0024 |
2 mg |
£ 108.20 |
Europe (EUR) | ||
04-0024 |
2 mg |
€ 126.37 |
Stemolecule Dorsomorphin dihydrochloride (also known as Compound C) is a potent inhibitor of AMP-activated protein kinase (AMPK) (Ki=109nM) and bone morphogenic protein (BMP) signaling1,2. It was identified in a screen for compounds that perturb dorsoventral axis formation in zebrafish3. Dorsomorphin functions through inhibition of BMP type I receptors ALK2, ALK3 and ALK6 and thus blocks BMP-mediated SMAD1/5/8 phosphorylation3. BMP signaling coordinates developmental patterning and have essential physiological roles in mature organisms4,5. Dorsomorphin has been used to probe BMP signaling in iron-hepcidin homeostasis, cardiomyogenesis and osteogenesis3,6,7.
Specifications
Product Name: Stemolecule Dorsomorphin
Catalog Number: 04-0024
Size: 2 mg
Alternate Name(s):
- Compound C
- 6-[4-(2-piperidin-1-ylethoxy)phenyl]-3-pyridin-4-ylpyrazolo[1,5-a]pyrimidine
Chemical Formula: C24H25N5O.2·HCl·H2O
Molecular Weight: 490.43
CAS Number: 866405-64-3
Purity: Greater than 99% by HPLC analysis
Formulation: Yellow solid
Solubility: For a 10 mM concentrated stock solution of Dorsomorphin, reconstitute the compound by adding 407.8 µL of DMSO to the entire contents of the vial. If precipitate is observed, warm the solution to 37 °C for 2 to 5 minutes. For cell culture, the media should be prewarmed prior to adding the reconstituted compound. Note: for most cells, the maximum tolerance to DMSO is less than 0.5%. This molecule is soluble in DMSO at 20 mM and water at 100 mM.
Storage and Stability: Store powder at 4 °C protected from light. Information about the stability of Stemolecules in solution is largely not available. As a general guideline, we recommend that stock solution be freshly made and stored in aliquots at −20 °C, protected from light. The effect of storage of stock solutions should be verified for each application.
Quality Control: The purity of Dorsomorphin was determined by HPLC analysis. The accurate mass was determined by mass spectrometry. Cellular toxicity of Dorsomorphin was tested on mouse embryonic stem cells.
Documents
Specification Sheets:
Safety Data Sheets:
Resources
- Gao, Y., Zhou, Y., Xu, A., and Wu, D. Effects of an AMP-activated protein kinase inhibitor, compound C, on adipogenic differentiation of 3T3-L1 cells. Biol Pharm Bull 31: 1716-1722 (2008).
- Anderson, G.J., and Darshan, D. Small-molecule dissection of BMP signaling. Nat Chem Biol 4: 15-16 (2008).
- Yu, P.B., Hong, C.C., Sachidanandan, C., Babitt, J.L., Deng, D.Y., Hoyng, S.A., Lin, H.Y., Bloch, K.D., and Peterson, R.T. Dorsomorphin inhibits BMP signals required for embryogenesis and iron metabolism. Nat Chem Biol 4: 33-41 (2008).
- Heisenberg, C.P., and Solnica-Krezel, L. Back and forth between cell fate specification and movement during vertebrate gastrulation. Curr Opin Genet Dev 18: 311-316 (2008).
- Cain, J.E., Hartwig, S., Bertram, J.F., and Rosenblum, N.D. Bone morphogenetic protein signaling in the developing kidney: Present and future. Differentiation 76: 831-842 (2008).
- Hao, J., Daleo, M.A., Murphy, C.K., Yu, P.B., Ho, J.N., Hu, J., Peterson, R.T., Hatzopoulos, A.K., and Hong, C.C. Dorsomorphin, a selective small molecule inhibitor of BMP signaling, promotes cardiomyogenesis in embryonic stem cells. PLoS One 3: e2904 (2008).
- Seib, F.P., Franke, M., Jing, D., Werner, C., and Bornhauser, M. Endogenous bone morphogenetic proteins in human bone marrow-derived multipotent mesenchymal stromal cells. Eur J Cell Biol 88: 257-271 (2009).
Additional Publications
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- Leibel SL; Winquist A; Tseu I; Wang J; Luo D; Shojaie S; Nathan N; Snyder E; Post M. Reversal of Surfactant Protein B Deficiency in Patient Specific Human Induced Pluripotent Stem Cell Derived Lung Organoids by Gene Therapy. Sci Rep 9:745 (2019).
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