Stemolecule™ Purmorphamine

04-0009

Brand: Stemolecule

Purmorphamine is a Smoothened agonist that promotes the differentiation of human and murine mesenchymal pr ogenitor cells into osteoblasts.

CAS Number: 483367-10-8
Alternate Name(s):
  • 2-(1-Naphthoxy)-6-(4-morpholinoanilino)- 9-cyclohexylpurine

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Product name Product code Pack size Price Price (USD) Price (GBP) Price (EUR)
Stemolecule™ Purmorphamine 04-0009 5 mg (select above) $ 128.00 £ 105.00 € 122.00

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Product Information

Purmorphamine is a small molecule that promotes the differentiation of human and murine mesenchymal progenitor cells into osteoblasts1,2. The mechanism of action study of Purmorphamine indicates that it is an agonist of Smoothened, a 7-transmembrane receptor of the hedgehog signaling pathway3,4. Purmorphamine can also be used to replace sonic hedgehog for the generation of motor neurons from human embryonic stem cells5.

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Stemgent and the Stemolecule brand name are trademarks of REPROCELL Inc., Japan.

Product Name: Stemolecule Purmorphamine

Catalog Number: 04-0009

Size: 5 mg

Alternate Name(s): 2-(1-Naphthoxy)-6-(4-morpholinoanilino)- 9-cyclohexylpurine

Chemical Formula: C31H32N6O2

Molecular Weight: 520.6

CAS Number: 483367-10-8

Purity: Greater than 96 % by HPLC analysis

Formulation: Pale beige solid

Solubility: For a 10 mM concentrated stock solution of Purmorphamine, reconstitute the compound by adding 960.4 µL of DMSO to the entire contents of the vial. If precipitate is observed, warm the solution to 37 °C for 2 to 5 minutes. For use in cell culture, warm the medium just prior to adding the reconstituted compound. Once the compound is added, mix and filter-sterilize the medium using a 0.2 uM low-protein binding filter. Purmorphamine is soluble in DMSO at 50 mM.

Storage and Stability: Store powder at 4 °C protected from light. Information about the stability of Stemolecules in solution is largely not available. As a general guideline, we recommend that stock solution be freshly made and stored in aliquots at −20 °C, protected from light. The effect of storage of stock solutions should be verified for each application.

Quality Control: The purity of Purmorphamine was determined by HPLC analysis. The accurate mass was determined by mass spectrometry. No acute cytotoxicity was observed in mouse embryonic stem cells following a 6 hour exposure to 1 nM — 100 uM of Purmorphamine.

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Safety Data Sheets:

  1. Wu, X., Ding, S., Ding, Q., Gray, N.S., and Shultz, P.G. A small molecule with osteogenesis-inducing activity in multipotent mesenchymal progenitor cells. J Am Chem Soc 124: 14520-14521 (2002).
  2. Beloti, M.M., Bellesini, L.S., and Rosa, A.L. Puromorphamine enhances osteogenic activity of human osteoblasts derived from bone marrow mesenchymal cells. Cell Biol Int 29: 537-541 (2005).
  3. Wu, X., Walker, J., Zhang, J., Ding, S., and Schultz, P.G. Purmorphamine induces osteogenesis by activation of the hedgehog signaling pathway. Chem Biol 11: 1229-1238 (2004).
  4. Sinha, S., and Chen, J.K. Purmorphamine activates the Hedgehog pathway by targeting Smoothened. Nat Chem Biol 2: 29-30 (2006).
  5. Li, X.J., Hu, B.Y., Jones, S.A., Zhang, Y.S., Lavaute, T., Du, Z.W., and Zhang, S.C. Directed differentiation of ventral spinal progenitors and motor neurons from human embryonic stem cells by small molecules. Stem Cells 26: 886-893 (2008).

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