Spheroid forming low adherence plasticware. Cell/protein repellent SP-polymer coating. Thousands of micro-wells laser-etched into the surface which generates massive numbers of uniform size embryoid bodies.
The EZSPHERE 35 mm Dish Type 903 is a product manufactured by Asahi Glass Company, for culturing of mammalian cells and the preparation of 3D cell spheroids. The dish size (diameter measurement) is designated in the product title, with "Type" referring to the configuration of the microwells etched into the plate. Each plate is individually laser-etched and provided in a sterile, ready-to-use format. The plates are made of polystyrene material that is coated with a biocompatible non-adherent surface. Mammalian cells will "slide" to bottom of the wells by gravity and aggregate with neighboring cells according to their biologically programmed narrative. EZSPHERE products are available in a variety of sizes and microwell configurations to suit your particular needs. The plates are an excellent way of creating iPS cell EB seed stocks for transferral to bioreactors such as the ABLE Bioreactor Magnetic Stir System or other suspension culture systems. No other plates or multiwell plate configurations can give you the yields and cost-performance of an EZSPHERE plate from AGC.
EZSPHERE Product Features
| Cat. No. | Product Description | Pack Size | Number of Wells | Length (µm) | Depth (µm) |
| AG4000-900 | EZSPHERE Dish 35 mm | 10 dishes | 2,300 | 500 | 200 |
| AG4010-900 | EZSPHERE Dish 60 mm | 10 dishes | 5,300 | 500 | 200 |
| AG4020-900 | EZSPHERE Dish 100 mm | 10 dishes | 14,000 | 500 | 200 |
| AG4000-902 | EZSPHERE Dish 35 mm Type 902 | 10 dishes | 2,300 | 500 | 200 |
| AG4000-903 | EZSPHERE Dish 35 mm Type 903 | 10 dishes | 1,000 | 800 | 400 |
| AG4000-904 | EZSPHERE Dish 35 mm Type 904 | 10 dishes | 600 | 800 | 300 |
| AG4810-900 | EZSPHERE Microplate 6 well | 5 plates | 2,400 | 500 | 20 |
| AG4860-900 | EZSPHERE Microplate 96 well | 5 plates | 80 | 500 | 200 |
Specifications
Product Name: EZSPHERE Dish 35 mm Type 903 (SP-polymer coating)
Catalog Number: AG4000-903SP
Size: 10 dishes
Storage and Stability: Store at Room Temperature
Sterility: Sterile
Notice To Purchaser: REPROCELL is a licensed AGC global distributor of EZSPHERE and EZ-Open Top Flask products everywhere, except for Japan.
Manufacturer: Asahi Glass Company (Japan)
Resources
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- Shimada H, Hashimoto Y, Nakada A, Shigeno K, Nakamura T, Accelerated generation of human induced pluripotent stem cells with retroviral transduction and chemical inhibitors under physiological hypoxia Biochemical and Biophysical Research Communications, Volume 417, Issue 2, Pages 659-664
- Aikawa N, Suzuki Y, Takaba K A Simple Protocol for the Myocardial Differentiation of Human iPS Cells Biological and Pharmaceutical Bulletin Vol. 38 (2015) No. 7 Pages 1070-1075
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- Hiroki Sato, Alimjan Idiris, Tatsuaki Miwa, Hiromichi Kumagai Microfabric Vessels for Embryoid Body Formation and Rapid Differentiation of Pluripotent Stem Cells Scientific Reports 6, Article number: 31063 (2016) doi:10.1038/srep31063
- Taku Nakayama, Shimpei Otsuka, Tatsuya Kobayashi, Hodaka Okajima, Kentaro Matsumoto, Yuichiro Hagiya, Keiji Inoue Taro Shuin, Motowo Nakajima, Tohru Tanaka, Shun-ichiro Ogura Dormant cancer cells accumulate high protoporphyrin IX levels and are sensitive to 5-aminolevulinic acid-based photodynamic therapy Scientific Reports 6, Article number: 36478 (2016) doi:10.1038/srep36478
- Ayako Aihara, Natsuki Abe, Koichiro Saruhashi, Tatsuro Kanaki, Taito Nishino Novel 3-D cell culture system for in vitro evaluation of anticancer drugs under anchorage-independent conditions Cancer Science Volume 107, Issue 12December 2016 Pages 1858−1866 DOI: 10.1111/cas.13095
- Waseem K. Raja, Alison E. Mungenast, Yuan-Ta Lin, Tak Ko, Fatema Abdurrob, Jinsoo Seo, Li-Huei Tsai Self-Organizing 3D Human Neural Tissue Derived from Induced Pluripotent Stem Cells Recapitulate Alzheimer's Disease Phenotypes PLOS ONE 11(9): e0161969. doi: 10.1371/journal.pone.0161969
- Hiroyuki Koike, Ran-Ran Zhang, Yasuharu Ueno, Keisuke Sekine, Yun-Wen Zheng, Takanori Takebe, Hideki Taniguchi Nutritional modulation of mouse and human liver bud growth through a branched-chain amino acid metabolism Development 2017 144: 1018-1024; doi: 10.1242/dev.143032