EZSPHERE™ Dish 35 mm Type 900

AG4000-900SP

Brand: EZSPHERE™ by Asahi Glass Company (AGC)

Purchase product: USA Store

Product Code Pack Size Price in
The Americas
Price in EMEA
AG4000-900SP 10 dishes USD $ 595.00 GBP £ 533.41 EUR € 605.43

Prices do not include shipping and handling charges.

ezsphere-dish-35-mm-type-900-sp-polymer-p301-88_medium

Micro-well specifications: 400-500 μm diameter, 100-200 μm deep, 2,300 micro-wells per dish.

Spheroid forming, low adherence plasticware. Cell/protein repellent SP-polymer coating. Thousands of micro-wells laser-etched into the surface which generates massive numbers of uniform size embryoid bodies.

Micro-well specifications: 400-500 µm diameter, 100-200 µm deep, 2700 micro-wells per dish

The EZSPHERE 35mm Dish Type 900 is a product manufactured by Asahi Glass Company, for culturing of mammalian cells and the preparation of 3D cell spheroids. The dish size (diameter measurement) is designated in the product title, with "Type" referring to the configuration of the microwells etched into the plate. Each plate is individually laser-etched and provided in a sterile, ready-to-use format. The plates are made of polystyrene material that is coated with a biocompatible non-adherent surface. Mammalian cells will "slide" to bottom of the wells by gravity and aggregate with neighboring cells according to their biologically programmed narrative. EZSPHERE products are available in a variety of sizes and microwell configurations to suit your particular needs. The plates are an excellent way of creating iPS cell EB seed stocks for transfer to bioreactors such as the ABLE Bioreactor Magnetic Stir System or other suspension culture systems. No other plates or multiwell plate configurations can give you the yields and cost-performance of an EZSPHERE plate from AGC.

Cat. No. Description Well Size (µm) Microwells per Dish/Well Pack Size
4000-900SP EZSPHERE SP dish, 35 mm 400-500 dia x 100-200 deep 2700 10
4010-900SP EZSPHERE SP dish, 60 mm 400-500 dia x 100-200 deep 6500 10
4020-900SP EZSPHERE SP dish, 100 mm 400-500 dia x 100-200 deep 17000 10
4810-900SP EZSPHERE SP Microplate, 6 well 400-500 dia x 100-200 deep 2700 5
4820-900SP EZSPHERE SP Microplate, 24 well 400-500 dia x 100-200 deep 470 5
4860-900SP EZSPHERE SP Microplate, 96 well 400-500 dia x 100-200 deep 95 5
4000-902SP EZSPHERE SP dish, 35 mm Type 902 500 dia x 200 deep 2700 10
4000-903SP EZSPHERE SP dish, 35 mm Type 903 800 dia x 400 deep 1250 10
4000-904SP EZSPHERE SP dish, 35 mm Type 904 800 dia x 300 deep 700 10

1536843730AGC_35mm_Dish_formated

Specifications

Product Name: EZSPHERE Dish 35mm Type 900 (SP-polymer coating)

Catalog Number: AG4000-900SP

Size: 10 dishes

Storage and Stability: Store at Room Temperature

Sterility: Sterile

Notice To Purchaser: REPROCELL is a licensed AGC global distributor of EZSPHERE and EZ-Open Top Flask products everywhere, except for Japan.

Manufacturer: Asahi Glass Company (Japan)

References

  1. Zhang RR, Takebe T, Miyazaki L, Takayama M, Koike H, Kimura M, Enomura M, Zheng YW, Sekine K, Taniguchi H Efficient Hepatic Differentiation of Human Induced Pluripotent Stem Cells in a Three-Dimensional Microscale Culture Stem Cells and Tissue Repair Methods in Molecular Biology Volume 1210, 2014, pp 131-141
  2. Shimada H, Hashimoto Y, Nakada A, Shigeno K, Nakamura T, Accelerated generation of human induced pluripotent stem cells with retroviral transduction and chemical inhibitors under physiological hypoxia Biochemical and Biophysical Research Communications, Volume 417, Issue 2, Pages 659-664
  3. Aikawa N, Suzuki Y, Takaba K A Simple Protocol for the Myocardial Differentiation of Human iPS Cells Biological and Pharmaceutical Bulletin Vol. 38 (2015) No. 7 Pages 1070-1075
  4. Matsuura K, Seta H, Haraguchi Y, Alsayegh K, Sekine H, Shimizu T, Hagiwara N, Yamazaki K, Okano T TRPV-1-mediated elimination of residual iPS cells in bioengineered cardiac cell sheet tissues Scientific Reports 6, Article number: 21747 (2016) doi:10.1038/srep21747
  5. Hiroki Sato, Alimjan Idiris, Tatsuaki Miwa, Hiromichi Kumagai Microfabric Vessels for Embryoid Body Formation and Rapid Differentiation of Pluripotent Stem Cells Scientific Reports 6, Article number: 31063 (2016) doi:10.1038/srep31063
  6. Taku Nakayama, Shimpei Otsuka, Tatsuya Kobayashi, Hodaka Okajima, Kentaro Matsumoto, Yuichiro Hagiya, Keiji Inoue Taro Shuin, Motowo Nakajima, Tohru Tanaka, Shun-ichiro Ogura Dormant cancer cells accumulate high protoporphyrin IX levels and are sensitive to 5-aminolevulinic acid-based photodynamic therapy Scientific Reports 6, Article number: 36478 (2016)doi:10.1038/srep36478
  7. Ayako Aihara, Natsuki Abe, Koichiro Saruhashi, Tatsuro Kanaki, Taito Nishino Novel 3-D cell culture system for in vitro evaluation of anticancer drugs under anchorage-independent conditions Cancer ScienceVolume 107, Issue 12December 2016 Pages 1858−1866 DOI: 10.1111/cas.13095
  8. Waseem K. Raja, Alison E. Mungenast, Yuan-Ta Lin, Tak Ko, Fatema Abdurrob, Jinsoo Seo, Li-Huei Tsai Self-Organizing 3D Human Neural Tissue Derived from Induced Pluripotent Stem Cells Recapitulate AlzheimerÕs Disease Phenotypes PLOS ONE 11(9): e0161969. doi: 10.1371/journal.pone.0161969
  9. Hiroyuki Koike, Ran-Ran Zhang, Yasuharu Ueno, Keisuke Sekine, Yun-Wen Zheng, Takanori Takebe, Hideki Taniguchi Nutritional modulation of mouse and human liver bud growth through a branched-chain amino acid metabolism Development 2017 144: 1018-1024; doi: 10.1242/dev.143032