Stemolecule Small Molecules provide small molecules used a in variety of experiments to inhibit or activate regulatory pathways important for stem cell biology. Each lot of Stemolecules is tested for cytotoxicity and purity. Stemolecules are packaged in convenient sizes to minimize the amount of weighing.
Doxycycline hyclate (dox) is a broad spectrum antibiotic derivative of tetracycline and an inhibitor of matrix metalloproteinases.
Stemgent hES Cell Cloning & Recover Supplement is a 1000× concentrate of Thiazovivin (2mM). In culture media it significantly improves the likelihood of primaryful sub-cloning from single cells, and increases attachment after passaging. This supplement is a ROCK inhibitor (see Thiazovivin, cat. # 04-0017 for more information).
04-0014 / 04-0014-10
A83-01 is a selective inhibitor of the transforming growth factor-beta (TGF-β) type I receptor ALK5, the Activin/Nodal receptor ALK4, and the nodal receptor ALK71.
ALK5 Inhibitor (also known as RepSox, E 616452, and SJN 2511) is a selective and ATP-competitive inhibitor of the TGF-β family type I receptor of activin receptor-like kinase (ALK5).
All-Trans Retinoic Acid (ATRA) is the oxidized form of Vitamin A, functioning as a signaling molecule for various developmental pathways that control differentiation and proliferation.
04-0004 / 04-0004-10 / 04-0004-02
CHIR99021 is a highly potent, specific and effective inhibitor of glycogen synthase kinase 3 beta (GSK-3β).
Cyclopamine is a steroid alkaloid isolated from the corn lily (Veratrum californicum) that is a Smoothened antagonist involved in both embryogenesis and cancer progression.
DAPT (a.k.a. GSI-IX or LY-374973) is a cell-permeable dipeptide that inhibits γ-secretase and indirectly inhibits Notch, γ -secretase substrate.
Dorsomorphin dihydrochloride (a.k.a. Compound C) is a potent inhibitor of AMP-activated protein kinase (AMPK) and bone morphogenic protein (BMP) signaling.
SRP002 / SRP002-2
A light-stable pan-RAR receptor agonist that maintains the same biological activity as ATRA (all-trans retinoic acid).
Forskolin is a natural product adenylate cyclase activator that increases cyclic AMP levels.
KAAD-cyclopamine is a sonic hedgehog antagonist that targets Smoothened, a 7-transmembrane receptor of the hedgehog signaling pathway.
04-0074 / 04-0074-10 / 04-0074-02
LDN193189 is a cell permeable, small molecule inhibitor of bone morphogenetic protein (BMP) type I receptors ALK2 and ALK3.
04-0006 / 04-0006-10 / 04-0006-02
PD03225901 inhibits mitogen-activated protein kinase (MAPK/ERK kinase or MEK) and demonstrates potential antineoplastic activity.
Purmorphamine is a Smoothened agonist that promotes the differentiation of human and murine mesenchymal pr ogenitor cells into osteoblasts.
04-0010 / 04-0010-05 / 04-0010-10
SB421542 is an inhibitor of the transforming growth factor-beta 1 (TGF-?1) activin receptor-like kinases (ALKs).
Sodium butyrate (butyric acid sodium salt) has been shown to direct the differentiation of mouse ESCs cells into hepatocytes.
Thiazovivin is a Rho-associated kinase (ROCK) inhibitor that protects human ESCs in the absence of ECM by regulating E-cadherin mediated cell-cell interaction. Also see ES Cell Cloning & Recovery Supplement – Thiazovivin (01-0014-500).
Valproic acid is a histone deacetylase (HDAC) inhibitor which improves reprogramming efficiency by at least 100 fold, and it is reported to regulate the differentiation and proliferation of various cell types.
Wnt Inhibitor IWP-2 prevents palmitylation of Wnt proteins by Porcupine (Porcn), a membrane-bound O-acyltransferase, thereby blocking Wnt secretion and activity. It also blocks phosphorylation of the Lrp6 receptor and accumulation of both Dvl2 and ?-catenin.
Wnt Inhibitor IWP-3 prevents palmitylation of Wnt proteins by Porcupine (Porcn), a membrane-bound O-acyltransferase, thereby blocking Wnt secretion and activity.
04-0036 / 04-0036-50
Wnt Inhbitor IWP-4 prevents palmitylation of Wnt proteins by Porcupine (Porcn), a membrane-bound O-acyltransferase, thereby blocking Wnt secretion and activity.
XAV939 is an inhibitor of the Wnt / ?-catenin pathway which modulates a number of stem cell behaviors.
04-0012 / 04-0012-10 / 04-0012-02
Y27632 is an inhibitor of Rho-associated kinase (ROCK) which is widely used to enhance survival of dissociated PSCs. It is common to supplement cell culture medium with 10 µM of ROCK Inhibit or during cell passage or while establishment of spheroids during the first 24 hours.