04-0016
Doxycycline hyclate (dox) is a broad spectrum antibiotic derivative of tetracycline and an inhibitor of matrix metalloproteinases.
01-0014-500
Stemgent hES Cell Cloning & Recover Supplement is a 1000× concentrate of Thiazovivin (2mM). In culture media it significantly improves the likelihood of primary sub-cloning of pluripotent stem cells from single cells, and increases attachment after passaging. This supplement is a ROCK inhibitor (see Thiazovivin, cat. # 04-0017 for more information).
04-0014 / 04-0014-10
A83-01 is a selective inhibitor of the transforming growth factor-beta (TGF-β) type I receptor ALK5, the Activin/Nodal receptor ALK4, and the nodal receptor ALK71.
04-0015
ALK5 Inhibitor (also known as RepSox, E 616452, and SJN 2511) is a selective and ATP-competitive inhibitor of the TGF-β family type I receptor of activin receptor-like kinase (ALK5).
04-0021
All-Trans Retinoic Acid (ATRA) is the oxidized form of Vitamin A, functioning as a signaling molecule for various developmental pathways that control differentiation and proliferation.
04-0004 / 04-0004-10 / 04-0004-02
CHIR99021 is a highly potent, specific and effective inhibitor of glycogen synthase kinase 3 beta (GSK-3β).
04-0022
Cyclopamine is a steroid alkaloid isolated from the corn lily (Veratrum californicum) that is a Smoothened antagonist involved in both embryogenesis and cancer progression.
04-0041
DAPT (a.k.a. GSI-IX or LY-374973) is a cell-permeable dipeptide that inhibits γ-secretase and indirectly inhibits Notch, γ -secretase substrate.
04-0024
Dorsomorphin dihydrochloride (a.k.a. Compound C) is a potent inhibitor of AMP-activated protein kinase (AMPK) and bone morphogenic protein (BMP) signaling.
SRP002 / SRP002-2
A light-stable pan-RAR receptor agonist that maintains the same biological activity as ATRA (all-trans retinoic acid).
04-0025
Forskolin is a natural product adenylate cyclase activator that increases cyclic AMP levels.
04-0028
KAAD-cyclopamine is a sonic hedgehog antagonist that targets Smoothened, a 7-transmembrane receptor of the hedgehog signaling pathway.
04-0074 / 04-0074-10 / 04-0074-02
LDN193189 is a cell permeable, small molecule inhibitor of bone morphogenetic protein (BMP) type I receptors ALK2 and ALK3.
04-0006 / 04-0006-10 / 04-0006-02
PD03225901 inhibits mitogen-activated protein kinase (MAPK/ERK kinase or MEK) and demonstrates potential antineoplastic activity.
04-0009
Purmorphamine is a Smoothened agonist that promotes the differentiation of human and murine mesenchymal pr ogenitor cells into osteoblasts.
04-0010 / 04-0010-10 / 04-0010-05
SB421542 is an inhibitor of the transforming growth factor-beta 1 (TGF-?1) activin receptor-like kinases (ALKs).
04-0005
Sodium butyrate (butyric acid sodium salt) has been shown to direct the differentiation of mouse ESCs cells into hepatocytes.
04-0017
Thiazovivin is a Rho-associated kinase (ROCK) inhibitor that protects human ESCs in the absence of ECM by regulating E-cadherin mediated cell-cell interaction. Also see ES Cell Cloning & Recovery Supplement – Thiazovivin (01-0014-500).
04-0007
Valproic acid is a histone deacetylase (HDAC) inhibitor which improves reprogramming efficiency by at least 100 fold, and it is reported to regulate the differentiation and proliferation of various cell types.
04-0034
Wnt Inhibitor IWP-2 prevents palmitylation of Wnt proteins by Porcupine (Porcn), a membrane-bound O-acyltransferase, thereby blocking Wnt secretion and activity. It also blocks phosphorylation of the Lrp6 receptor and accumulation of both Dvl2 and ?-catenin.
04-0035
Wnt Inhibitor IWP-3 prevents palmitylation of Wnt proteins by Porcupine (Porcn), a membrane-bound O-acyltransferase, thereby blocking Wnt secretion and activity.
04-0036 / 04-0036-50
Wnt Inhbitor IWP-4 prevents palmitylation of Wnt proteins by Porcupine (Porcn), a membrane-bound O-acyltransferase, thereby blocking Wnt secretion and activity.
04-0046
XAV939 is an inhibitor of the Wnt / ?-catenin pathway which modulates a number of stem cell behaviors.
04-0012 / 04-0012-10 / 04-0012-02
Y27632 is an inhibitor of Rho-associated kinase (ROCK) which is widely used to enhance survival of dissociated PSCs. It is common to supplement cell culture medium with 10 µM of ROCK Inhibit or during cell passage or while establishment of spheroids during the first 24 hours.
