CI-huOB Immortalized Human Osteoblasts

CI-huOB are immortalized human osteoblasts. Osteoblasts are specialized cells primarily responsible for bone formation and bone mineralization. They produce and secrete the organic components of the bone matrix, including collagen, which provides the framework for deposition of calcium and other minerals, the so-called mineralization.

CI-huOBs can be expanded in standard monolayer culture but undergo differentiation when cultured in our huOB Differentiation Medium (INS-ME-1007), where they deposit calcium and initiate mineralization. When combined with 3D cell culture conditions, CI-huOBs even form so-called mini-bones.

Characteristics

• Positive for alkaline phosphatase activity
• Calcium deposits (Alizarin Red staining)
• Long term spheroid 3D culture

Culture Conditions

• Culture Medium: huOB Maintenance Medium for CI-huOB Osteoblasts (Cat No. INS-ME-1006)
• Differentiation Medium: huOB Differentiation Medium for CI-huOB Osteoblasts (Cat No. INS-ME-1007)
• Culture Matrix: not required
• Cryopreservation Medium: Freezing Solution for Immortalized Cell Lines (Cat No. INS-SU 1004)

CI-huOB stained with Phalloidin and DAPI.

Buying and Ordering the Cells

For non-profit organizations, cells are available for perpetual academic research under the Limited Research Use License. Please contact us about licenses for commercial organizations.

inscreenex company name and inscreenex brand name are the property of inscreenex GmbH, Germany.

Product Name

CI-huOB

Product Description

 Immortalized Human Osteoblasts

Strain

CI-huOB

Catalog Number

INS-CI-1005

Donor Information

• Race: Caucasian
• Sex: Female
• Age: 63 years old

Precryopreservation Culture Conditions

• Culture Medium: huOB Maintenance Medium for CI-huOB Osteoblasts (Cat No. INS-ME-1006)
• Differentiation Medium: huOB Differentiation Medium for CI-huOB Osteoblasts (Cat No. INS-ME-1007)
• Culture Matrix: not required
• Cryopreservation Medium: Freezing Solution for Immortalized Cell Lines (Cat No. INS-SU 1004)
• BSL 1

Size

1 vial, 0.5 × 10⁶ cells per vial

Storage

Store in liquid nitrogen vapor phase.

Quality Control

• Viability: >75%
• Bacteria: Negative
• Fungi: Negative
• Mycoplasma: Negative
• Virus (HIV1&2, HBV, HCV): Negative

Sterility

Sterile

Notice To Purchaser

This product is intended for Research Use Only.  Commercial Use may require additional agreements. Please contact us for more information.