Freezing Medium for Immortalized Cell Lines

Medium for Cryopreservation of Immortalized Cells

This product is a replacement for INS-SU-1004.

This freezing medium is optimized for the cryopreservation of Inscreenex immortalized cells. This freezing medium is supplied pre-mixed and ready-to-use. Freezing medium is optimized for high viability, and it contains DMSO and serum.

Cryopreservation of Immortalized Cells

1. Aspirate medium.
2. Wash with PBS and aspirate PBS.
3. Add Trypsin/EDTA (TE) solution to the cells and incubate at room temperature or 37 °C for 5-10min, or until the cells detach.
4. Examine the cells under a microscope. When the cells start to detach, gently tap the side of the vessel to loosen the remaining cells.
5. Resuspend cells in 2% FBS in PBS and transfer to a 15 mL conical bottom tube.
6. Centrifuge cells at 200 × g for 4 min.
7. Aspirate supernatant and gently resuspend the cell pellet in Freezing medium (approx. 1 million cells/mL).
8. Transfer cell suspension into cryovial(s) and place them into a freezing container (“Mr.Frosty” or similar).
9. Place the freezing container at −80 °C for 16-24 h.
10. Transfer cryovials to liquid nitrogen vapor for long-term storage.

inscreenex company name and inscreenex brand name are the property of inscreenex GmbH, Germany.

Product Name

Freezing Medium

Catalog Number

INS-SU-1027

Size

30 mL

Storage and Stability

Store Freezing Medium at −20 °C. Medium is stable until the expiration date listed on the bottle if stored as indicated.

Sterility

Sterile

Notice To Purchaser

REPROCELL is an authorized distributor of select cell culture products from inscreenex.