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CI-huBroBEC Immortalized Human Bronchial Basal Epithelial Cells

INS-CI-1025

Brand: inscreenex®

Immortalized human bronchial basal epithelial cells.

Currency: 

Product name Catalog number Pack size Price Price (USD) Price (GBP) Price (EUR)
CI-huBroBEC Immortalized Human Bronchial Basal Epithelial Cells INS-CI-1025 1 vial (select above) $ 2,200.00 £ 1,760.00 € 2,156.00

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Note: prices shown do not include shipping and handling charges.

CI-huBroBECs are immortalized bronchial basal epithelial cells. Bronchial epithelial cells line the respiratory airways, including the bronchi and bronchioles, where they act as a defensive barrier and maintain the airway structure. CI-huBroBECs show a basal-like phenotype in standard monolayer conditions and can be differentiated into a pseudostratified airway epithelium in ALI culture conditions, where they form ciliated cells, as well as mucus-producing goblet-like cells.

CI-huBroBECs are a newer and improved variant of our CI-huAEC cells, which they directly replace. CI-huBroBECs were derived from CI-huAECs by single-cell cloning.

Characteristics

  • TEER of >1500 Ω×sqcm in Air-Liquid-Interface culture
  • Tight junctions (ZO1)
  • Basal-like in 2D monolayer (TP63, KRT5)
  • Differentiation in ALI (CFTR, MUC5, Acetylated Tubulin)
  • Susceptible to SARS-CoV-2 infection

CI-huBroBEC stained with Phalloidin and DAPI.

Buying and Ordering the Cells

For non-profit organizations, cells are available for perpetual academic research under the Limited Research Use License. Please contact us about licenses for commercial organizations.


inscreenex company name and inscreenex brand name are the property of inscreenex GmbH, Germany.

Product Name

CI-huBroBEC

Product Description

 Immortalized Human Bronchial Basal Epithelial Cells

Strain

CI-huBroBEC

Passage Number

Passage 7

Catalog Number

INS-CI-1025

Donor Information

  • Race: Caucasian
  • Sex: Female
  • Age: 20 years old

Precryopreservation Culture Conditions

  • Culture Medium: huBroBEC Medium (Cat No. INS-ME-1025)
  • Culture Matrix: Epithelial Cell Coating Solution  (Cat. No.INS-SU-1020-100ml)
  • Cryopreservation Medium: Freezing Solution for Immortalized Cell Lines (Cat. No. INS-SU-1004)
  • BSL 1

Size

1 vial, 1 × 106 cells per vial

Storage

Store in liquid nitrogen vapor phase.

Quality Control

  • ICC for ZO-1, KRT5, TP63: Pass
  • TEER: >1000 Ω×cm2
  • Viability: >90%
  • Bacteria: Negative
  • Fungi: Negative
  • Mycoplasma: Negative
  • Virus (HIV1&2, HBV, HCV): Negative

Sterility

Sterile

Notice To Purchaser

This product is intended for Research Use Only.  Commercial Use may require additional agreements. Please contact us for more information.