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CI-huArlo Immortalized Human Alveolar Epithelial Cells

INS-CI-1031

Brand: inscreenex®

Immortalized human alveolar epithelial cells.

Currency: 

Product name Catalog number Pack size Price Price (USD) Price (GBP) Price (EUR)
CI-huArlo Immortalized Human Alveolar Epithelial Cells INS-CI-1031 1 vial (select above) $ 2,200.00 £ 1,760.00 € 2,156.00

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Note: prices shown do not include shipping and handling charges.

CI-huArlo cells are immortalized human alveolar epithelial cells. The cell line is an improved version of CI-hAELVi alveolar epithelial cell line. It was derived from CI-huALEVi by single-cell printing, and it shows an overall improved alveolar phenotype, most notably a tighter barrier function. 

While the cells do show an AT-1 phenotype in standard culture conditions, they adopt certain AT-2 characteristics in extended ALI culture conditions including secretion of surfactants, formation of microvilli and lamellar structures).

There is a newer and improved alveolar cell line available, CI-huArlo, which is a direct derivative of CI-hAELVi.

This CI-hAELVi cell line is the result of a collaboration between HIPS, Saarbrücken (Germany), and inscreenex.

Characteristics

  • TEER of >1500 Ω/sqcm in Liquid-Liquid-Interface culture
  • TEER of >2000Ω/sqcm in Air-Liquid-Interface culture
  • Tight junctions (ZO1, Occludin)
  • Monolayer growth on semipermeable Inserts.
  • Susceptible to SARS-CoV-2 infection

Culture Conditions

  • Culture Medium: huAEC Medium Kit for Alveolar Cells (Cat No. INS-ME-1013)
  • Culture Matrix: huAEC Coating  (Cat. No. INS-SU-1018-100ml)
  • Cryopreservation Medium: Freezing Solution for Immortalized Cell Lines (Cat. No. INS-SU-1004)

CI-huArlo stained with Phalloidin and DAPI.

Buying and Ordering the Cells

For non-profit organizations, cells are available for perpetual academic research under the Limited Research Use License. Please contact us about licenses for commercial organizations.


inscreenex company name and inscreenex brand name are the property of inscreenex GmbH, Germany.

Product Name

CI-huArlo

Product Description

Immortalized Human Alveolar Epithelial Cells

Strain

CI-huArlo

Catalog Number

INS-CI-1031

Donor Information

  • Not available

Precryopreservation Culture Conditions

  • Culture Medium: huAEC Medium Kit for Alveolar Cells (Cat No. INS-ME-1013)
  • Culture Matrix: huAEC Coating  (Cat. No. INS-SU-1018-100ml)
  • Cryopreservation Medium: Freezing Solution for Immortalized Cell Lines (Cat. No. INS-SU-1004)
  • BSL 1

Size

1 vial, 5 × 105 cells per vial

Storage

Store in liquid nitrogen vapor phase.

Quality Control

  • ICC for occludin, ZO-1: Pass
  • TEER: >1000 Ω×cm2
  • Viability: >80%
  • Bacteria: Negative
  • Fungi: Negative
  • Mycoplasma: Negative
  • Virus (HIV1&2, HBV, HCV): Negative

Sterility

Sterile

Notice To Purchaser

This product is intended for Research Use Only.  Commercial Use may require additional agreements. Please contact us for more information.