CI-hAELVi Immortalized Human Alveolar Epithelial Cells

CI-hAELVi cells are immortalized human alveolar epithelial cells. Alveolar epithelial cells play a crucial role in the respiratory system by lining the alveoli, tiny air sacs within the lungs where gas exchange occurs. CI-hAELVis have been extensively used to model the air-blood-barrier in vitro.

While the cells do show an AT-1 phenotype (expression of Caveolin, no surfactant secretion) in standard culture conditions, they adopt certain AT-2 characteristics in extended ALI culture conditions including secretion of surfactants, formation of microvilli and lamellar structures).

There is a newer and improved alveolar cell line available, CI-huArlo, which is a direct derivative of CI-hAELVi.

This CI-hAELVi cell line is the result of a collaboration between HIPS, Saarbrücken (Germany), HZI, Braunschweig (Germany), and inscreenex.

Characteristics

• TEER of >1500 Ω/sqcm in Liquid-Liquid-Interface culture
• TEER of >2000Ω/sqcm in Air-Liquid-Interface culture
• Tight junctions (ZO1, Occludin)
• Susceptible to SARS-CoV-2 infection

Culture Conditions

• Culture Medium: huAEC Medium Kit for Alveolar Cells (Cat. No. INS-ME-1013)
• Culture Matrix: huAEC Coating  (Cat. No. INS-SU-1018-100ml)
• Cryopreservation Medium: Freezing Solution for Immortalized Cell Lines (Cat. No. INS-SU-1004)

CI-hALEVi stained with Phalloidin and DAPI.

Buying and Ordering the Cells

For non-profit organizations, cells are available for perpetual academic research under the Limited Research Use License. Please contact us about licenses for commercial organizations.

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inscreenex company name and inscreenex brand name are the property of inscreenex GmbH, Germany.

Product Name

CI-hAELVi

Product Description

Immortalized Human Alveolar Epithelial Cells

Strain

CI-hAELVi

Passage Number

Passage 7

Catalog Number

INS-CI-1015

Donor Information

• Race: Caucasian
• Sex: Male

Precryopreservation Culture Conditions

• Culture Medium: huAEC Medium Kit for Alveolar Cells (Cat. No. INS-ME-1013)
• Culture Matrix: huAEC Coating  (Cat. No. INS-SU-1018-100ml)
• Cryopreservation Medium: reezing Solution for Immortalized Cell Lines (Cat. No. INS-SU-1004)
• BSL 1

Size

1 vial, 5 × 10⁵ cells per vial

Storage

Store in liquid nitrogen vapor phase.

Quality Control

• ICC for Caveolin-1, occludin, ZO-1, pan-Cytokeratin, Cytokeratin183: Pass
• TEER: >1000 Ω×cm²
• Viability: >75%
• Bacteria: Negative
• Fungi: Negative
• Mycoplasma: Negative
• Virus (HIV1&2, HBV, HCV): Negative

Sterility

Sterile

Notice To Purchaser

This product is intended for Research Use Only.  Commercial Use may require additional agreements. Please contact us for more information.