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CI-hAELVi Immortalized Human Alveolar Epithelial Cells

INS-CI-1015

Brand: inscreenex®

Immortalized human alveolar epithelial cells.

Currency: 

Product name Catalog number Pack size Price Price (USD) Price (GBP) Price (EUR)
CI-hAELVi Immortalized Human Alveolar Epithelial Cells INS-CI-1015 1 vial (select above) $ 2,200.00 £ 1,760.00 € 2,156.00

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Note: prices shown do not include shipping and handling charges.

CI-hAELVi cells are immortalized human alveolar epithelial cells. Alveolar epithelial cells play a crucial role in the respiratory system by lining the alveoli, tiny air sacs within the lungs where gas exchange occurs. CI-hAELVis have been extensively used to model the air-blood-barrier in vitro.

While the cells do show an AT-1 phenotype (expression of Caveolin, no surfactant secretion) in standard culture conditions, they adopt certain AT-2 characteristics in extended ALI culture conditions including secretion of surfactants, formation of microvilli and lamellar structures).

There is a newer and improved alveolar cell line available, CI-huArlo, which is a direct derivative of CI-hAELVi.

This CI-hAELVi cell line is the result of a collaboration between HIPS, Saarbrücken (Germany), HZI, Braunschweig (Germany), and inscreenex.

Characteristics

  • TEER of >1500 Ω/sqcm in Liquid-Liquid-Interface culture
  • TEER of >2000Ω/sqcm in Air-Liquid-Interface culture
  • Tight junctions (ZO1, Occludin)
  • Susceptible to SARS-CoV-2 infection

Culture Conditions

  • Culture Medium: huAEC Medium Kit for Alveolar Cells (Cat. No. INS-ME-1013)
  • Culture Matrix: huAEC Coating  (Cat. No. INS-SU-1018-100ml)
  • Cryopreservation Medium: Freezing Solution for Immortalized Cell Lines (Cat. No. INS-SU-1004)

CI-hALEVi stained with Phalloidin and DAPI.

Buying and Ordering the Cells

For non-profit organizations, cells are available for perpetual academic research under the Limited Research Use License. Please contact us about licenses for commercial organizations.


inscreenex company name and inscreenex brand name are the property of inscreenex GmbH, Germany.

Product Name

CI-hAELVi

Product Description

Immortalized Human Alveolar Epithelial Cells

Strain

CI-hAELVi

Passage Number

Passage 7

Catalog Number

INS-CI-1015

Donor Information

  • Race: Caucasian
  • Sex: Male

Precryopreservation Culture Conditions

  • Culture Medium: huAEC Medium Kit for Alveolar Cells (Cat. No. INS-ME-1013)
  • Culture Matrix: huAEC Coating  (Cat. No. INS-SU-1018-100ml)
  • Cryopreservation Medium: reezing Solution for Immortalized Cell Lines (Cat. No. INS-SU-1004)
  • BSL 1

Size

1 vial, 5 × 105 cells per vial

Storage

Store in liquid nitrogen vapor phase.

Quality Control

  • ICC for Caveolin-1, occludin, ZO-1, pan-Cytokeratin, Cytokeratin183: Pass
  • TEER: >1000 Ω×cm2
  • Viability: >75%
  • Bacteria: Negative
  • Fungi: Negative
  • Mycoplasma: Negative
  • Virus (HIV1&2, HBV, HCV): Negative

Sterility

Sterile

Notice To Purchaser

This product is intended for Research Use Only.  Commercial Use may require additional agreements. Please contact us for more information.