Brightase-C
NP892-431, NP892-432
Brand: Nippi
Brightase-C is a recombinant Grimontia hollisae collagenase that is expressed in Brevibacillus.
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Currency:
| Product name | Product code | Pack size | Price | Price (USD) | Price (GBP) | Price (EUR) |
|---|---|---|---|---|---|---|
| Brightase-C (40 mg) | NP892-431 | 40 mg | (select above) | $ 268.00 | £ 216.00 | € 253.00 |
| Brightase-C (2 × 40 mg) | NP892-432 | 2 × 40 mg | (select above) | $ 534.00 | £ 430.00 | € 502.00 |
Note: prices shown do not include shipping and handling charges.
Product Information
Tissue Dissociating enzyme for isolating primary cells
Brightase-C is a recombinant Grimontia hollisae collagenase that is expressed in Brevibacillus. Unlike the commonly used C. histolyticum collagenase, Brightase-C can cleave sequences with glutamic acid and degrade undenatured type VI collagen. Brightase-C is highly purified to remove contaminating proteinases, yielding improved run-to-run and lot-to-lot reproducibility.
Brightase-C and Brightase-TH were used in combination to digest mouse pancreas and isolate functional beta cells.
No animal products are used in the production of Brightase-C.
NIPPI company name and logo is the property of NIPPI Inc., Japan.
Product Name
Brightase-C Recombinant Collagenase
Catalog Number
NP892-431: 40 mg
NP892-432: 2x40 mg (80 mg total)
Formulation
Lyophilized from 2 mL of a 20 mg/mL solution in 20 mM HEPES, pH 7.5, 30 mM NaCl, 2 mM CaCl2
Storage and Stability
Store at −20 °C soon after arrival. Thaw before use.
Stock Solution
Prepare a stock solution by adding 2 mL Sterile Water per vial and agitating gently at 4 °C. Divide stock solutions into single-use aliquots and store at −20 °C. Avoid repeated freezing and thawing.
Working solutions should contain ≥1 mM CaCl2 for optimal activity and stability.
Quality Control
- Activity: ≥6,000 U/mg
- Endotoxin: ≤10 EU/mg
Notice To Purchaser
This product is for research use only, not for therapeutic or diagnostic purposes. It is not allowed to sell this product to a third party or use it for commercial purposes without permission from REPROCELL.
- Tanaka, K., Okitsu, T., Teramura, N. et al. Recombinant collagenase from Grimontia hollisae as a tissue dissociation enzyme for isolating primary cells. Scientific Reports volume 10, 3927 (2020). DOI: 10.1038/s41598-020-60802-z