Human Keratinocyte Growth Supplement (HKGS)

Human Keratinocyte Growth Supplement (HKGS) is a sterile, concentrated (100X) solution intended for use with EpiLife™ medium or Medium 154 to culture human epidermal keratinocytes.

HKGS is an ionically-balanced supplement containing bovine pituitary extract (BPE), recombinant human insulin-like growth factor-I, hydrocortisone, bovine transferrin, and human epidermal growth factor. Each 5-mL bottle of HKGS contains all of the growth factors, hormones, and tissue extracts necessary for the culture of human epidermal keratinocytes and is the correct amount of supplement for a 500-mL bottle of medium.

When a 500-mL bottle of medium is supplemented with HKGS, the final concentrations of the components in the supplemented medium are:

• Bovine pituitary extract (BPE): 0.2% v/v
• Recombinant human insulin-like growth factor-I: 0.01 μg/mL
• Hydrocortisone: 0.18 μg/mL
• Bovine transferrin: 5 μg/mL

• Human epidermal growth factor: 0.2 ng/mL

For Research Use Only. Not for use in diagnostic procedures.

 Gibco™ is part of ThermoFisher.

• Cell Type: Keratinocyte Cells
• Concentration: 100 X
• For Use With (Application): Mammalian Cell Culture, Primary Cell Culture
• Product Line: Cascade Biologics
• Quantity: 5 mL
• Source: Human
• Species: Human
• Form: Liquid
• Product Type: Growth Supplement
• Sterility: Sterile
• Unit Size: Each
• Contents & Storage: Store in freezer (–5 to –20°C).

Safety Data Sheet:

• S0015 – Human Keratinocyte Growth Supplement (HKGS)