FibColl® Atelocollagen Inserts 24

FibColl® Atelocollagen Insert 24 is a novel cell culture insert, sized to fit a standard 24-well plate,  that uses a membrane entirely made from atelocollagen. Unlike other collagen-coated plastic membranes, FibColl® pores are not clogged, and the fiber structure of atelocollagen enables cell culture in an in vivo mimicking environment. The microporous structure between atelocollagen fibrils allows the permeation of molecules even over 600 kDa, making it suitable for barrier function assessment and co-culture models.

Example: Epithelial Tight Junctions

Immunostaining (red: ZO-1); blue: nuclear

TEER measurement

TEER Results

Epithelial cells form tight junctions that restrict the flow of ions and molecules between the luminal and basement membrane sides of the cell layer.  Canine kidney tubular epithelial cells (MDCK cells) were cultured for 2 weeks on FibColl.  During the course of the culture, tight junctions formed as shown the development of Transepithelial Electrical Resistance (TEER), which indicates that the free flow of ions across the epithelial barrier was impeded.

KOKEN company name and logo and AteloCell brand are the property of KOKEN Corp. Ltd., Japan.

Product Name: FibColl Atelocollagern Inserts 24

Catalog Number: KKN-FAI-24

Size: Pack of 24 Inserts

Insert Dimensions:

Insert: 19mm dia x 16mm
Membrane: 6.4mm dia x 35 µm

Storage and Stability: Store at Room Temperature

Sterility: Sterile

Notice To Purchaser: REPROCELL is a licensed global distributor of KOKEN’s collagen-derived products everywhere, except for Japan.

Manufacturer: Koken Co., Ltd. (Japan)

Brochure:

FibColl® Atelocollagen Inserts 24 — high permeability ateocollagen insert for 24 well plate