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Off-the-shelf hypoimmune cell lines

REPROCELL has developed high-quality HLA knockout iPSC lines using StemEdit—our state-of-the-art genome editing technology, utilizing a proprietary nuclease system. is also well-suited for clinical gene-editing projects.

We offer the following two products to meet the research needs of our customers:

StemEdit™ Human iPSC non-HLA class 1/2 (B2M/CIITA Homo double KO)

Benefits of StemEdit™ human iPSCs

By using our cell lines, you can immediately start your research using HLA knockout iPSCs without the hassle of editing iPSC lines yourself. In both cell lines, either one or both alleles (homozygous) of the target genes (B2M, CIITA) are knocked out and this reliably suppresses HLA expression.

The introduction of the new hypoimmune StemEdit™ Human iPSC lines marks a significant milestone in advancing immune research and regenerative medicine. By enabling researchers to study and manipulate immune cell interactions with unprecedented precision, these cell lines exemplify REPROCELL's commitment to empowering scientific discovery. We will continue to innovate and provide world-class tools to support breakthroughs in cell therapy and immunology.

— Dr. Chikafumi Yokoyama, CEO of REPROCELL Inc.

The Science of StemEdit™ human iPSCs

StemEdit human iPSCs grown in vitro stain positive for standard pluripotency markers

StemEdit Human iPSC non-HLA class 1 (B2M Homo KO) and non-HLA class 1/2 (B2M/CIITA Homo double KO) cells were cultured using NutriStem hPSC XF Medium (01-0005) on iMatrix-511 (NP892-011). Pluripotency for both cell lines was confirmed by the strong expression of Nanog, Oct3/4, and SSEA4.

Below: (A.) stained B2M Homo KO cells; (B.) B2M/CIITA Homo double KO cells.

A

B

StemEdit™human iPSCs exhibit the expected karyotype

Karyotyping of StemEdit Human iPSC non-HLA class 1/2 (B2M/CIITA Homo double KO)(RCRP043E) and StemEdit Human iPSC non-HLA class 1 (B2M Homo KO)" (RCRP044E) was performed, and both strains were found to have normal karyotypes (46, XX).

Confirmation of HLA class 1 expression suppression

HLA class 1 expression levels in both strains and the parent strain (VCT-37-F35) were evaluated by flow cytometry analysis using an anti-HLA-ABC antibody. HLA class I expression was confirmed in the parent strain, and HLA class I expression was significantly suppressed in B2M/CIITA Homo double KO (RCRP043E) and B2M Homo KO (RCRP044E), where the B2M gene was knocked out.

Related Products

The items below are all available from the REPROCELL product catalog:

Sartorius® NutriStem™ hPSC XF
Culture Medium

Matrixome® iMatrix™-511
Stem Cell Culture Substrate