iMatrix-511 Stem Cell Culture Substrate

iMatrix-511 is a highly purified and refined laminin-511 E8 fragments, produced in CHO cells.

iMatrix-511 features make it an ideal matrix for pluripotent stem cell culture:

• Promotes greater stem cell adhesion than all other matrix proteins that have been tested
• Easy to use (liquid format)
• E8 fragments retain integrin binding specificity and capacity and display higher potency than natural Laminin-511
• Equivalent performance, but lower cost than the legacy iMatrix-511 product

Laminin is localized to the basement membrane and plays a key role in cell adhesion and proliferation. Laminin-511 (α5, β1 laminin) binds to integrin α6β1 to promote cell signaling. Laminin-511 provides an ideal matrix for the proliferation of a wide variety of cell types including stem and iPS cells.

iMatrix-511 is functionally equivalent to iMatrix-511 SILK (Cat. No. NP892-021). The only difference is the expression system used for bioproduction. The iMatrix-511 SILK product is produced in recombinant silkworm cocoon, while iMatrix-511  is produced in CHO-S cells. Both are E8 fragments that have been purified using the same processes.

Specifications

Product Name: iMatrix-511 Stem Cell Culture Substrate

Catalog Numbers:

• NP892-011
• NP892-012

Sizes:

• NP891-011: 2 × 175 µg
• NP891-012: 6 × 175 µg

Molecular Weight: 150 kDa

Purity: > 95 % pure

Formulation: Purified Laminin-511 E8 proteolytic fragment

Storage and Stability: Store at 4 °C and protect from light exposure. Stable for 2 years from manufacturing date.

Concentration: 500 µg/mL

Source: CHO cells

Quality Control: Integrin binding K_d < 10 nM

Sterility: Sterile

Notice To Purchaser: REPROCELL is a licensed distributor of Matrixome cell culture substrates to the global market.

Recommended Usage: iMatrix-511 is suitable for use as a substrate for culture of various cell types, including ES/iPS cells.

Manufacturer: Matrixome Corporation (Japan)

Documents

Safety Data Sheet:

• NP892-01 Safety Data Sheet

Specifications Sheets:

• NP892-01 Specifications Sheet

Product Flyers:

• iMatrix™ Cell Culture Substrates
• iMatrix™ 511 Cell Culture Substrate
  

Protocols:

• Nippi iMatrix-511 Cell Culture Substrate: Use with Stemgent® StemRNA™ 3^rd Gen Reprogramming Kit and NutriStem™ hPSC XF Culture Medium

References

1. Takayama K. et al., "Laminin 411 and 511 promote the cholangiocyte differentiation of human induced pluripotent stem cells". Biochemical and Biophysical Research Commun.474 (1): 91-96 (2016).
2. Nishimura K. et al., "Estradial facilitates functional integration of iPSC-derived dopaminergic neurons into striatal neuronal circuits via activation of integrin a5b1". Stem Cell Reports6  (4): 511-524 (2016).
3. Matsuno K. et al., "Redefining definitive endoderm subtypes by robust induction of human induced pluripotent stem cells". Differentiation2016.04.002.
4. Hayashi R. et al., "Co-ordinated ocular development from human iPS cells and recovery of corneal function". Nature531, 368-80 (2016),
5. Sasaki K. et al., "Robust in vitro induction of human germ cell fate from pluripotent stem cells". Cell Stem Cell 17 (2):178-194 (2015).
6. Okumura N. et al., "Laminin-511 and -521 enable efficient in vitro expansion of human corneal endothelial cells". Invest Ophthalmal Vis Sci.56 (5), 2933-42 (2015).
7. Nakagawa M. et al., "A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells". Scientific Reports 4: 3594 (2014).
8. Miyazaki T. et al. "Laminin E8 fragments support efficient adhesion and expansion of dissociated human pluripotent stem cells." Nature Communications 3: 1236 (2012).
9. Taniguchi Y. et al., "The C-terminal region of laminin β-chains modulates the integrin-binding affinities of laminins." J. Biol. Chem.284 (12): 7820-31 (2009).
10. Ido H. et al., "The requirement of the glutamic acid residue at the third position from the carboxyl termini of the laminin gamma-chains in integrin-binding by laminins." J. Biol. Chem.282 (15): 11144-54 (2017).

Additional Publications

• Bakare AB; Meshrkey F; Lowe B; Molder C; Rao RR; Zhan J; Iyer S. MitoCellPhe reveals mitochondrial morphologies in single fibroblasts and clustered stem cells. Am J Phys Cell Physiol 321:C735-748 (2021).
• Meshrkey F; Ayuso AC; Rao RR; Iyer S. Quantitative analysis of mitochondrial morphologies in human induced pluripotent stem cells for Leigh syndrome. Stem Cell Res 57:102572 (2021).
• Devito LG; Cooper F; D'Angelo I; Smith J; Healy L. Generation of FOUR iPSC lines (CRICKi004-A; CRICKi005-A; CRICKi006-A, CRICKi007-A) from Spinal muscle atrophy patients with lower extremity dominant (SMALED) phenotype. Stem Cell Res 65:102954 (2022).
• Supakul S; Leventoux N; Tabuchi J; Mimura M; Ito D; Maeda S; Okano H. Establishment of KEIOi005-A iPSC line from urine-derived cells (UDCs) of a mild Alzheimer’s disease (AD) donor with multiple risk SNPs for sporadic Alzheimer’s disease (sAD). Stem Cell Res 62:102802 (2022).
• Rajasingh S; Sigamani V; Selvam V; Gurusamy S; Kirankumar S; Vasanthan J; Rajasingh J. Comparative analysis of human induced pluripotent stem cell-derived mesenchymal stem cells and umbilical cord mesenchymal stem cells. J Cell Mol Med 25:8904 (2021).
• Fiacco E; Alowaysi M; Astro V; Adamo A. Derivation of two naturally isogenic iPSC lines (KAUSTi006-A and KAUSTi006-B) from a mosaic Klinefelter Syndrome patient (47-XXY/46-XY). Stem Cell Res 49:102049 (2020).
• Alowaysi M; Fiacco E; Astro V; Adamo A. Establishment of iPSC lines from a high-grade Klinefelter Syndrome patient (49-XXXXY) and two genetically matched healthy relatives (KAUSTi003-A, KAUSTi004-A, KAUSTi004-B, KAUSTi005-A, KAUSTi005-B, KAUSTi005-C). Stem Cell Res 49:102008 (2020).
• Shimada M; Tsukada K; Kagawa N; Matsumoto Y. Reprogramming and differentiation-dependent transcriptional alteration of DNA damage response and apoptosis genes in human induced pluripotent stem cells. J Radiation Res 2019:1-10 (2019).
• Nakajima M; Yoshimatsu S; Sato T; Nakamura M; Okahara J; Sasaki E; Shiozawa S; Okano H. Establishment of induced pluripotent stem cells from common marmoset fibroblasts by RNA-based reprogramming. Biochem Biophys Research Commun in press:https://doi.org/10.1016/j.bbrc.2019.05.175 (2019).
• Liu L-P; Li Y-M; Guo N-N; LI S; Ma X; Zhang Y-X; Gao Y; Huang J-L; Zheng D-X; Wang L-Y; Xu H; Hui L; Zheng Y-W. Therapeutic Potential of Patient iPSC-Derived iMelanocytes in Autologous Transplantation. Cell Reports 27:455-466.e5 (2019).
• Harmanto Y; Maki T; Yakagi Y; Miyamoto S; Takahashi J. Xeno‐free culture for generation of forebrain oligodendrocyte precursor cells from human pluripotent stem cells. J Neuro Res 2019:1-18 (2019).
• Tsujimara T; Takase O; Yoshikawa M; Sano E; Hayashi M; Hoshi K; Takato T; Toyoda A; Okano H; Hishikawa K. Controlling gene activation by enhancers through a drug-inducible topological insulator. bioRxiv http://dx.doi.org/10.1101/534073 (2019).