StemEdit
Next-Level Gene Editing Service

From Research to Clinical Translation

StemEdit is REPROCELL’s advanced genome editing platform for iPSCs, designed to support programs from early research through clinical development. REPROCELL uses this platform to enable partners to kick-start their gene-editing-based stem cell projects toward the clinic. Begin with research-grade editing and seamlessly transition to clinical-grade iPSC lines without restarting your cell line development.

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AI-Designed Precision Editing

StemEdit leverages OpenCRISPR-1™, a de novo AI-designed gene editor created using large language models trained on extensive CRISPR-Cas datasets. The system is engineered for performance in human cells, and it demonstrates:

• High on-target editing efficiency
• Reduced off-target activity
• Lower predicted immunogenicity

These features are especially valuable in both preclinical research and clinical-stage programs, where precision and safety are critical.

OpenCRISPR-1™ is licensed from Profluent Bio. See press release, REPROCELL Launches StemEdit: Clinical Gene Editing Services and New iPSC Lines Using AI-Designed Editing System

StemEdit: Custom Gene Editing Services for iPSCs

StemEdit provides tailored genome editing services to generate the precise genetic modifications your project requires. Programs can begin with research-grade editing and screening, then, when ready, transition seamlessly into clinical-grade iPSC development using aligned processes and documentation.

Capabilities & Deliverables

StemEdit enables a wide range of complex genetic modifications in iPSCs with confidence, reproducibility, and full traceability.

Our services support both research and clinical translation, delivering precisely engineered cell lines tailored to your project needs.

Capabilities include:

• Knock-in of large gene fragments (e.g., reporters, regulatory elements via landing pad technology)
• Knock-in of biallelic mutations for disease models or functional studies
• Knock-out of single or multiple genes

StemEdit Service Overview & Deliverables

StemEdit provides end-to-end genome editing services for iPSCs, supporting programs from research through clinical translation. Our workflow ensures precise, reproducible edits, full characterization, and deliverables aligned with downstream clinical requirements.

Step-by-Step Process

1. Project Scoping & Design
   • Align objectives and target edits with research and clinical goals
2. Guide & Editor Optimization
   • AI- and bioinformatics-driven designs for precise on-target editing with minimal off-target effects
3. Execution
   • Delivery into iPSCs and gene editing
   • Deep sequencing and off-target profiling (WGS with at least 50×)
4. Clone Selection & Validation
   • Phenotypic and genotypic quality control
   • Comprehensive reporting for parental and edited iPSC lines
5. Documentation & Support
   • Deliverables structured for downstream clinical applications
   • Support for regulatory compliance and seamless transition to clinical-grade lines