StemEdit™ Human iPSC non-HLA class 1 (B2M Homo KO)
RCRP044E
Brand: StemEdit™
Human iPSCs with a Knockout in the B2M (HLA Class I) gene
Currency:
| Product name | Catalog number | Pack size | Price | Price (USD) | Price (GBP) | Price (EUR) |
|---|---|---|---|---|---|---|
| StemEdit™ Human iPSC non-HLA class 1 (B2M Homo KO) | RCRP044E | 1 × 10⁶ cells | (select above) | $ Ask | £ Ask | € Ask |
Note: prices shown do not include shipping and handling charges.
Recommended Products
- NutriStem™ hPSC XF Culture Medium for Human iPS and ES Cells 01-0005 / 01-0005-100
- iMatrix-511 Stem Cell Culture Substrate NP892-011 / NP892-012
Ready-to-use Research-Grade HLA Class I knockout iPSCs generated using REPROCELL’s StemRNA™ Reprogramming Technology.
These cells feature all the advantages of our RNA reprogramming technology, including no retention of reprogramming factors and easy culture in NutriStem hPSC XF Medium (01-0005). They have been engineered to contain a B2M (HLA Class I) knockout mutation, which is carefully characterized and offers significant time savings compared to creating your own reprogrammed and edited cell lines.
Explore our other StemEdit™ Research iPSCs lines.
The parent line, StemRNA Clinical iPSC Clone VCT-37-F35, is also available for clinical and research use. Please contact us for more info: info-us@reprocell.com.
| Cat. No.: | RCRP044E |
| Parent Strain ID |
StemRNA Clinical iPSC Clone VCT-37-F35 |
| Donor Race: | Caucasian |
| Donor Sex: | Female |
| Donor Age: | 23 |
| Reprogramming Technology: | StemRNA Reprogramming Technology |
| Tissue Source: | Skin (Fibroblasts) |
| Karyotype: | Normal (46 XX) |
| Microbiology: | Negative for bacteria, virus, mycoplasma |
StemEdit Human iPSC non-HLA class 1 (B2M Homo KO) cells were cultured in NutriStem hPSC XF Medium (01-0005) on iMatrix-511 (NP892-011) and stained with pluripotency markers. Robust expression of Nanog, Oct3/4, and SSEA4 confirmed pluripotency.
Analysis of HLA Class I expression. HLA Class I expression (Red Curve) is measured by flow cytometry. Both the B2M Mutant (middle panel) and the Double Mutant (right panel) demonstrate strong suppression of HLA Class I expression compared to the Wild Type cells (left panel).
The Role of B2M
In humans, the B2M protein is required for the presentation of HLA proteins A-G on the cell surface. Disruption of the B2M gene reduces T-cell activation and interferes with the T-cell-mediated immune response through inhibition of Class I HLA signaling.
Stemgent and the StemEdit brand name are trademarks of REPROCELL Inc., Japan.
Product Name: StemEdit Human iPSC non-HLA class 1 (B2M Homo KO)
Product Description: Human iPS Cells established from fibroblasts, reprogrammed with REPROCELL's StemRNA Reprogramming technology, with a knockout in the B2M (HLA Class I) gene
Parental Strain ID: StemRNA Clinical iPSC Clone VCT-37-F35
Tissue Source: Skin (fibroblasts)
Catalog Number: RCRP044N
Donor Information
- Race: Caucasian
- Sex: Female
- Age: 23 years old
Precryopreservation Culture Conditions
- Culture Medium: NutriStem® hPSC XF Culture Medium (Cat. No. 01-0005)
- Culture Matrix: iMatrix-511 (Cat. No. NP892-011)
- Cryopreservation Medium: NutriFreez™ D10 Freezing Medium (Cat. No. 01-0020-50)
Size: 1 vial, 1 × 106 cells per vial
Storage: Store in liquid nitrogen vapor phase.
Quality Control
- Pluripotency by ICC: Pass
- Viability: > 80 %
- Bacteria: Negative
- Mycoplasma: Negative
- Virus (HIV1&2, HBV, HCV, HTLV1&2, Parvovirus B19): Negative
- Karyotype: 46, XX
Sterility: Sterile
Notice To Purchaser: This product is intended for Research Use Only. Commercial Use may require additional agreements. Please contact info-us@reprocell.com for more information. This Product is not intended for Clinical or Diagnostic Use.