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Stemgent StemAb Oct4 Antibody (Affinity Purified), Rabbit anti-Mouse/Human

Code: 09-0023-base
  • $197.00
  • (Delivery from $41.50)
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Oct4 (Octamer-4, also known as POU5F1) is a homeodomain transcription factor of the POU family which is involved in the regulation of pluripotency during normal development. The expression of Oct4 is associated with an undifferentiated phenotype in embryonic stem (ES) cells, while gene knockdown of Oct4 promotes differentiation. As such, it is frequently used as a marker for undifferentiated ES cells or induced pluripotent stem (iPS) cells. Oct4 is one of the key transcription factors used to reprogram mouse and human fibroblasts to a pluripotent state. The Oct4 Antibody (Affinity Purified)was screened on human and mouse ES cells using immunocytochemistry and flow cytometry and selected as the best Oct4 antibody available for researchers needing to demonstrate pluripotency.

 

 

Oct4 staining of human ES cells

 

Immunocytochemistry analysis of Oct4 on H1 human ES cells. Cells were treated with StemAb Oct4 Antibody (Affinity Purified) using a 1:100 dilution followed by a secondary Cy3 conjugated antibody (red). DAPI staining was performed to visualize nuclei (blue).

FACS Analysis of Oct4-stained H1 cells

Flow cytometry analysis of Oct4 on H1 human ES cells. Purple histogram represents Oct4 Antibody and open histogram represents isotype control at the same concentration.

Product Name

StemAb Oct4 Antibody (Affinity Purified), Rabbit anti-Mouse/Human

Catalog Number

09-0023

Size

100 µL

Formulation

Phosphate-buffered solution, pH 7.4, 1% BSA and 0.02% sodium azide

Storage and Stability

Store at 4 °C protected from light. Do not freeze. Stable for 6 months from date of receipt when stored as directed.

Quality Control

Tested by immunocytochemistry and flow cytometry to ensure product quality.

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Recommended Usage

The suggested use of this antibody is a 1:100 dilution for immunocytochemistry and a 1:5 dilution for flow cytometry. For an application specific protocol, please see Protocol: Immunocytochemistry and Protocol: Immunoflourescence Staining of Cells for Flow Cytometry.

Isotype

Rabbit IgG

Immunogen

Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of human Oct4

Reactivity

Mouse, Human

Preparation

The antibody was purified by immunogen affinity chromatography.

Clone

Polyclonal

  1. Looijenga, L.H., Stoop, H., de Leeuw, H.P., de Gouveia Brazao, C.A., Gillis, A.J., van Roozendaal, K.E., van Zoelen, E.J., Weber, R.F., Wolffenbuttel, K.P., van Dekken, H., Honecker, F., Bokemeyer, C., Perlman, E.J., Schneider, D.T., Kononen, J., Sauter, G., Oosterhuis, J.W. POU5F1 (OCT3/4) identifies cells with pluripotent potential in human germ cell tumors. Cancer Res 63: 2244-2250(2003).
  2. Zaehres, H., Lensch, M.W., Daheron, L., Stewart, S.A., Itskovitz-Eldor, J., Daley, G.Q. High-efficiency RNA interference in human embryonic stem cells. Stem Cells 23: 299-305(2005).
  3. Takahashi, K., and Yamanaka, S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126, 663-676(2006).
  4. Takahashi, K., Tanabe K., Ohnuki, M., Narita, M., Ichisaka T., Tomoda K. and Yamanaka, S. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131: 861-872(2007).
  5. Park, I.H., Arora, N., Huo, H., Maherali, N., Ahfeldt, T., Shimamura, A., Lensch, M.W., Cowan, C., Hochedlinger, K., and Daley, G.Q. Disease-specific induced pluripotent stem cells. Cell 134: 877-886(2008).

Additional Publications

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