StemAb™ Alkaline Phosphatase Staining Kit II

00-0055

Brand: Stemgent™

Purchase product: USA Store

Product Code Pack Size Price in NCSA Price in EMEA
00-0055 50 assays USD $ 97.00 GBP £ 74.79 EUR € 84.88

Prices do not include shipping and handling charges.

stemab-alkaline-phosphatase-staining-kit-ii-p194-78_medium

Alkaline phosphatase (AP) is expressed at high levels in pluripotent stem cells. AP dephosphorylates nucleotides, proteins, and alkaloids under alkaline conditions. After staining, undifferentiated cells appear red or purple whereas differentiated cells appear colorless.

The undifferentiated state of embryonic stem (ES) and induced pluripotent stem (iPS) cells can be characterized by a high level of alkaline phosphatase (AP) expression which, along with the expression of other surface markers, indicates undifferentiated cells with the potential to self-renew. AP is a hydrolase enzyme responsible for dephosphorylating molecules such as nucleotides, proteins, and alkaloids under alkaline conditions. When fixed ES or iPS cells are stained using the AP Staining Kit, undifferentiated cells appear red or purple, whereas differentiated cells appear colorless.

Specifications

Product Name: StemAb Alkaline Phosphatase Staining Kit II

Catalog Number: 00-0055

Size: 50 assays

Storage and Stability: Store at 4 °C. Do not freeze. Stable for 6 months from date of receipt when stored as directed.

Quality Control: StemAb Alkaline Phosphatase Staining Kit II is functionally tested on human iPS cells and mouse ES cells to ensure product quality.

Kit Contents:

  • Fix Solution: 25 mL (Cat. No. 09-0042)
  • AP Staining Solution A: 10 mL (Cat. No. 09-0043)
  • AP Staining Solution B: 10 mL (Cat. No. 09-0065)
  • AP Staining Solution C: 10 mL (Cat. No. 09-0066)

Documents

Protocols

References

  1. Pease, S., Braghetta, P., Gearing, D., Grail, D., and Williams, R.L. (1990) Isolation of embryonic stem (ES) cells in media supplemented with recombinant leukemia inhibitory factor (LIF). Dev Biol. 141: 344-352.
  2. Smith, A.G., Nichols, J., Robertson, M., and Rathjen, P.D. (1992) Differentiation inhibiting activity (DIA/LIF) and mouse development. Dev. Biol. 151: 339-351.
  3. Takahashi, K., Tanabe K., Ohnuki, M., Narita, M., Ichisaka T., Tomoda K. and Yamanaka, S. (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131: 861-872.
  4. Yu, J., Vodyanik, M.A., Smuga-Otto, K., Antosiewicz-Bourget, J., Frane, J.L., Tian, S., Nie, J., Jonsdottir, G.A., Ruotti, V., Stewart, R., Slukvin, I.I., and Thomson, J.A. (2007) Induced pluripotent stem cell lines derived from human somatic cells. Science 318: 1917-1920.

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