The mammalian Krüppel-like transcription factor 4 (Klf4) is an evolutionarily conserved zinc finger-containing transcription factor with diverse regulatory functions in cell growth, proliferation, differentiation and embryogenesis. It also plays a significant role in the process of tumorigenesis. Klf4 is one of the key transcription factors that have been used to reprogram mouse and human fibroblasts to induced pluripotent stem (iPS) cells.
Immunocytochemistry analysis of Klf4 on NIH3T3 cells transfected with a mouse Klf4 expression construct (left) and HeLa cells transfected with a human Klf4 expression construct (right). Two days post transfection cells were stained with Klf4 Antibody (Purified) at a 1:100 dilution followed by a secondary Cy 3 antibody (red). DAPI staining was performed to visualize nuclei (blue).
Klf4 Antibody (Purified), Mouse anti-Mouse/Human
Phosphate-buffered solution, pH 7.2, 0.1% sodium azide, and 0.2% BSA
Storage and Stability
Store at 4 °C protected from light. Do not freeze. Stable for 6 months from date of receipt when stored as directed.
Tested by immunocytochemistry to ensure product quality.
The suggested use of this antibody is a 1:100 dilution for immunocytochemistry. For an application specific protocol, please see Protocol: Immunocytochemistry.
Mouse IgG1, κ
Human Klf4 recombinant protein
This antibody was purified by affinity chromatography.