Stemolecule Doxycycline hyclate (dox) is a broad spectrum antibiotic derivative of tetracycline and an inhibitor of matrix metalloproteinases1. Tetracycline-controlled transcriptional activation is a method of inducible expression whereby transcription is reversibly turned on or off in the presence of tetracycline or one of its derivatives such as dox2. For this type of reprogramming method, dox-inducible lentiviral reagents are used to induce the expression of virally transduced genes and generate induced pluripotent stem (iPS) cells from somatic cells3-7.
Stemolecule Doxycycline Hyclate
(2Z,4S,5S,6R,12aS)-2-[amino(hydroxy)methylidene]-4-(dimethylamino)-5,10,11,12a-tetrahydroxy-6-methyl-4a,5,5a,6-tetrahydro-4H-tetracene-1,3,12-trione; ethanol; hydrochloride
C22H24N2O8· HCl · ½(H2O) · ½(C2H6O)
Greater than 98% by TLC analysis
Yellow to yellow with a green cast powder
For a 10 mM stock solution of doxycycline hyclate, reconstitute the compound by adding 1.95 mL of water to the entire contents of the vial.
Storage and Stability
Store powder at 4 °C protected from light. Information about the stability of Stemolecules in solution is largely not available. As a general guideline, we recommend that stock solution be freshly made and stored in aliquots at −20 °C, protected from light. The effect of storage of stock solutions should be verified for each application.
The purity of doxycycline hyclate was determined by TLC analysis. The accurate mass was determined by mass spectrometry. Cellular toxicity of doxycycline hyclate was tested on mouse embryonic stem cells.