Gene Editing Services

Thanks to the discovery of CRISPR-Cas9, gene editing is more accessible than ever before. However, some genetic modifications remain challenging and there are even more factors to consider if your cells are intended for clinical use.

CRISPR: Clustered Regularly Interspaced Short Palindromic Repeats.

By outsourcing your clinical gene editing to REPROCELL, you can achieve the genotype you need before moving on to expensive cell bank manufacturing processes.

By evaluating the percentage of target cells and determining their transfection efficiency (go/no-go decision point) StemEdit saves time as the SNIPER pre-screen is performed before laborious clone selection.

SNIPER: Specification of Newly Integrated Position and Exclusion of Random-integration.

Our StemEdit clinical gene editing service uses advanced CRISPR-SNIPER gene editing technology to develop your engineered stem cells. Due to the increased screening specificity of CRISPR-SNIPER, we can successfully achieve complex genetic edits with high accuracy at an early stage.

❯ About CRISPR-SNIPER gene editing technology

Note: CRISPR-SNIPER modified cells are developed, manufactured or supplied by GenAhead Bio Inc. under license from ERS Genomics Limited and Broad Institute.

HLA expression was measured by flow cytometry in iPS cell lines with double knockout of B2M and CIITA genes. Since the regulation of Class II expression occurs in post-differentiated cells, the expression of Class II is compared in macrophages induced to differentiate from iPS cells (right: using anti-HLA-DR antibody). The expression of HLA of both Classes is suppressed in the B2M/CIITA knockout line. These results indicate that the generation of iPS cells to suppress the immune rejection is possible with genome editing technology. Other gene editing services are also available, so please contact us if you have any requests using the inquiry form below.

Since HLA's mediate the immunological response to therapeutic cells, these B2M/CIITA knockout iPSCs are a perfect place to start the development of cellular therapeutics.

Creating hypoimmune clinical iPSCs by B2M/CIITA double knock-out

HLA Class I and II expression in B2M/CIITA double knock-out hypoimmune cells. HLA expression was measured by flow cytometry in unedited parental iPSC line and after knock out of B2M and CIITA genes. Expression of HLA I and II is suppressed in B2M/CIITA knockout lines. Results indicate that the generation of iPSC for the purpose of suppressing an immune rejection is feasible with Crispr SNIPER gene editing technology.

(A.) HLA Class I expression was measured in the parental iPSC-derived macrophages by flow cytometry.

(B.) Undifferentiated iPSCs (“Parental iPSCs”) do not express HLA II. Expression of HLA Class II occurs only in cells post differentiation. Expression of HLA II gene HLA-DR was therefore measured in iPSC-derived macrophages.