Embryonic (ES) and induced pluripotent stem cells (iPSCs) are renowned for their fastidious nature. Grown without antibiotics and requiring delicate handling, it can take years to master the art of iPSC culture. It takes a high degree of operator skill to create and care for these cells, but selecting the right products can make it easier.
In this article, we have listed our customers' top 10 stem cell culture products. These products are available for purchase in both Europe and the US via our website.
1. Stemfect RNA Transfection Kit
Our Stemfect RNA Transfection Kit has been favored by researchers for years. Boasting a 90% transfection efficiency for several cell types, it is perhaps unsurprising that the kit is so popular among industry scientists and academics. A variety of RNA molecules can be transfected using this reagent, which has been used in dozens of publications to date.
2. StemRNA 3rd Gen Reprogramming Kit
The StemRNA 3rd Gen Reprogramming Kit is one of the most efficient footprint-free technologies on the market. This kit can create iPSCs from skin, blood, and even urine. Cells are ready to pick from just 10 days and no screening is required due to the absence of DNA or viral vectors.
3. Nutristem hPSC XF Culture Medium
NutriStem is a GMP-grade culture medium designed to support the feeder-free culture of stem cells. It has been used to support a variety of iPSC-derived cells, including cardiomyocytes, neurons, hepatocytes, and endothelial progenitor cells. A Drug Master File for NutriStem is available to support clinical applications.
4. StemRNA Neuro - Human neurons
Created by the company that first commercialized iPSC-derived cardiomyocytes and hepatocytes, StemRNA Neuro is the latest offering by REPROCELL. Formerly ReproNeuro, these cells are matured in just 2 weeks and are available from healthy and Alzheimer's donors.
5. Stemolecule CHIR99021
Stemolecule CHIR99021 is our most popular small molecule. It is the most selective inhibitor of GSK-3β reported to date but does not exhibit cross-reactivity against cyclin-dependent kinases. By blocking GSK-3β, CHIR99021 enables the self-renewal of embryonic stem cells.
6. Stemfactor FGF-basic, human recombinant
Stemfactor bFGF is a ~17 kDa protein consisting of 155 amino acid residues. FGF-basic is added to ES cell culture medium to maintain cells in their undifferentiated state. Members of the FGF superfamily play a central role during prenatal development, postnatal growth, and tissue regeneration by promoting cellular proliferation and differentiation.
7. iMatrix 511 Stem Cell Culture Substrate
iMatrix-511 stem cell culture substrate is our recommended matrix for iPSC and ESC culture. This liquid matrix contains purified E8 fragments that retain integrin binding specificity and capacity, and display higher potency than natural Laminin-511.
8. Primate ES Cell Medium
Our primate ES cell medium is the gold standard for feeder-dependant stem cell cultures. Simply add bFGF before use, if needed - no mixing required. Each lot of our primate ES cell medium is functionally tested using human iPSCs and is tested for osmolarity, pH, sterility, and Mycoplasma.
9. Stemolecule Y27632
Y27632 is a cell-permeable small molecule that is widely used in naive stem cell protocols. This ROCK inhibitor enhances the survival and cloning efficiency of dissociated human ES cells without affecting their self-renewal properties or pluripotency.
10. ABLE Biott Bioreactor System
The ABLE bioreactor is a lab-scale system that allows researchers to culture stem cells more efficiently. It takes five hours to culture two billion human iPSCs using the ABLE Bioreactor - a feat that would take 24 hours using manual cell culture.
About the author
Zara Puckrin BSc, digital marketing manager, REPROCELL Europe
Zara is a GCU graduate who loves minimalism, marketing, and molecular biology. You can contact her on LinkedIn.
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