StemAb™ Nanog Antibody (Affinity Purified), Rabbit anti-Mouse/Human

09-0020

Brand: Stemgent™

Purchase product: USA Store

Product Code Pack Size Price in NCSA Price in EMEA
09-0020 100 μL USD $ 285.00 GBP £ 220.64 EUR € 250.43

Prices do not include shipping and handling charges.

stemab-nanog-antibody-affinity-purified-rabbit-anti-mouse-human-p263-404_medium

Nanog is a member of the homeobox family of DNA binding transcription factors that has been shown to maintain embryonic stem (ES) cell self-renewal independently of leukemia inhibitory factor (LIF) and Stat3. Nanog mRNA is present in pluripotent mouse and human cell lines and is absent from differentiated cells. Functionally, Nanog works together with other key pluripotent factors (Oct4, Sox2, and Lin28) to reprogram human fibroblasts and generate induced pluripotent stem (iPS) cells. These key factors form a regulatory network to support or limit each other's expression level, maintaining the properties of ES cells. The Nanog Antibody (Affinity Purified) was screened on human and mouse ES cells using immunocytochemistry and selected as the best Nanog antibody available for researchers needing to demonstrate pluripotency.

Specifications

Product Name: StemAb Nanog Antibody (Affinity Purified), Rabbit anti-Mouse/Human

Catalog Number: 09-0020

Size: 100 µL

Formulation: Phosphate-buffered solution, pH 7.2, 1% BSA, 20% glycerol, and 0.01% thimerosal

Storage and Stability: Store at 4 °C protected from light. Do not freeze. Stable for 6 months from date of receipt when stored as directed.

Quality Control: Tested by immunocytochemistry to ensure product quality.

Recommended Usage: For immunocytochemistry, the suggested use of this antibody is a 1:100 dilution. For an application specific protocol, please see Protocol: Immunocytochemistry.

Concentration: 0.5 mg/mL

Isotype: Rabbit IgG

Immunogen: Recombinant protein fragment containing a sequence corresponding to a region within amino acids 109 and 300 of human Nanog

Reactivity: Mouse, Human

Preparation: This antibody was purified by antigen affinity chromatography.

Clone: Polyclonal

Documents

Protocols

References

  1. Mitsui K; Tokuzawa Y; Itoh H; Segawa K; Murakami M; Takahashi K; Maruyama M; Maeda M; Yamanaka S. The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells. Cell 113:631-640 (2003).

Additional Publications