StemRNA™ Human iPSC 771-3G

Ready to use IPS Cells made using the Cutting-Edge StemRNA 3^rd Gen Reprogramming Technology

 Taking full advantage of the power of the  3rd Gen Technology, these cells feature:

• No possibility of integration or retention of Reprogramming Vectors
• Immediate thawing into NutriStem hPSC XF Culture medium (Cat. No. 01-0005) in iMatrix-511 substrate (Cat. No. NP892-011) with easy conversion to other standard iPSC Media.
• The possibility of saving significant time compared to reprogramming your own cells.

RPChiPS7713G1 Cells were cultured in NutriStem hPSC XF Medium
on iMatrix-511 and stained for common pluripotency markers and DAPI.

Specifications

Product Name: StemRNA Human iPSC 771-3G

Catalog Number: RCRP005N

Product Description: Human iPS Cells established from endothelial progenitor cells (derived from peripheral blood), reprogrammed with the Stemgent StemRNA 3^rd Gen Reprogramming technology

Strain: RPChiPS7713G1

Passage Number: Passage 15

Donor Information:

• Race: Caucasian
• Sex: Male
• Age: 32 years old

Precryopreservation Culture Conditions:

• Culture Medium: NutriStem® hPSC XF Culture Medium (Cat. No. 01-0005)
• Culture Matrix: iMatrix-511 (Cat. No. NP892-011)
• Cryopreservation Medium: NutriFreez^TM D10 Cryopreservation Medium (Cat. No. 01-0020-50)

Size: 1 vial, 1 × 10⁶ cells per vial

Storage: Store in liquid nitrogen vapor phase.

Quality Control

• Pluripotency by ICC: Pass
• Viability: > 80 %
• Bacteria: Negative
• Mycoplasma: Negative
• Virus (HIV1&2, HBV, HCV, HTLV1&2, Parvovirus B19): Negative
• Karyotype: 46, XY

Sterility: Sterile

Notice To Purchaser: This product is intended for Research Use Only.  Commercial Use may require additional agreements. Please contact info-us@reprocell.com for more information.

Documents

Product Flyer:

• StemRNA™ Human iPSCs

Specification Sheets:

• RCRP005N Specifications Sheet

Protocol for Thawing and Plating:

• Protocol for Thawing and Plating

Lot Datasheets / CoA:

• Lot J1901-07 Datasheet

References

Additional Publications

• Francois L; Boskovic P; Knerr J; Je W; Sigismondao G; Schwan C; More TH; Schlotter M; Krijgsveld J; Hiller K; Grosse R; lichter P; Radlwimmer B. BCAT1 redox function maintains mitotic fidelity. Cell Reports 41:111524 (2022).
• Shimada H; Sato Y; Sasaki T; Shimozawa A: imaizumi K; Shindo T; Miyao S; Kiyama K; Kondo T; Shibata S; Ishii S; Kuromitsu J; Aoyagi H; Ito D; Okano H. A next-generation iPSC-derived forebrain organoid model of tauopathy with tau fibrils by AAV-mediated gene transfer. Cell Reports Methods 2:100289 (2022).
• Park G; Shin M; Lee W; Hotta A; Kobayashi T; Kosodo Y. Direct visualization of the transition status during neural differentiation by dual-fluorescent reporter human pluripotent stem cells. Stem Cell Reports 17:1903 (2022).