StemEdit™ Human iPSC non-HLA class 1/2 (B2M/CIITA Homo double KO)
RCRP043E
Brand: StemEdit™
Human iPSCs with Knockouts in the B2M (HLA Class I) and CIITA (HLA Class II) genes.
Currency:
| Product name | Catalog number | Pack size | Price | Price (USD) | Price (GBP) | Price (EUR) |
|---|---|---|---|---|---|---|
| StemEdit™ Human iPSC non-HLA class 1/2 (B2M/CIITA Homo double KO) | RCRP043E | 1 × 10⁶ cells | (select above) | $ Ask | £ Ask | € Ask |
Note: prices shown do not include shipping and handling charges.
Recommended Products
- NutriStem™ hPSC XF Culture Medium for Human iPS and ES Cells 01-0005 / 01-0005-100
- iMatrix-511 Stem Cell Culture Substrate NP892-011 / NP892-012
Ready to use Research-Grade HLA Class I/Class II double knockout iPSCs made using REPROCELL’s RNA Reprogramming Technology.
These cells feature all the advantages of our RNA reprogramming technology, including no retention of reprogramming factors and easy culture in NutriStem hPSC XF Medium (01-0005). They have been engineered to contain a B2M (HLA Class I) and CIITA (HLA Class II) double knockout mutation, which is carefully characterized and offers significant time savings compared to creating your own reprogrammed and edited cell lines.
The parent line, StemRNA Clinical iPSC Clone VCT-37-F35, is also available for clinical and research use. Please contact us for more info: info-us@reprocell.com.
| Cat. No.: | RCRP043E |
| Parent Strain ID |
StemRNA Clinical iPSC Clone VCT-37-F35 |
| Donor Race: | Caucasian |
| Donor Sex: | Female |
| Donor Age: | 23 |
| Reprogramming Technology: | StemRNA Reprogramming Technology |
| Tissue Source: | Blood (EPCs) |
| Karyotype: | Normal (46 XX) |
| Microbiology: | Negative for bacteria, virus, mycoplasma |
StemEdit Human iPSC non-HLA class 1/2 (B2M/CIITA Homo double KO) cells were cultured in NutriStem hPSC XF Medium(01-0005) on iMatrix-511 (NP892-011) and stained with pluripotency markers. Robust expression of Nanog, Oct3/4, and SSEA4 confirmed pluripotency.
Analysis of HLA Class I expression. HLA Class I expression (Red Curve) is measured by flow cytometry. Both the B2M Mutant (middle panel) and the Double Mutant (right panel) show strong suppression of HLA Class I expression compared to the Wild Type cells (left panel).
The Role of B2M and CIITA.
In humans, the B2M protein is required for the presentation of HLA proteins A-G on the cell surface. Disruption of the B2M gene reduces T-cell activation and interferes with the T-cell-mediated immune response through inhibition of Class I HLA signaling. CIITA regulates the expression of the HLA Class II complex. Knocking out CIITA leads to reduced activity of HLA Class II and suppression of the immune response.
Stemgent and the StemEdit brand name are trademarks of REPROCELL Inc., Japan.
Product Name: : StemEdit Human iPSC non-HLA class 1/2 (B2M/CIITA Homo double KO)
Product Description: Human iPS Cells established from fibroblasts, reprogrammed with REPROCELL's StenRNA Reprogramming technology, with knockouts in the B2M (HLA Class I) and CIITA (HLA Class II) genes.
Parental Strain ID: StemRNA Clinical iPSC Clone VCT-37-F35
Tissue Source: Skin (fibroblasts)
Catalog Number: RCRP043E
Donor Information
- Race: Caucasian
- Sex: Female
- Age: 23 years old
Precryopreservation Culture Conditions
- Culture Medium: NutriStem® hPSC XF Culture Medium (Cat. No. 01-0005)
- Culture Matrix: iMatrix-511 (Cat. No. NP892-011)
- Cryopreservation Medium: NutriFreez™ D10 Freezing Medium (Cat. No. 01-0020-50)
Size: 1 vial, 1 × 106 cells per vial
Storage: Store in liquid nitrogen vapor phase.
Quality Control
- Pluripotency by ICC: Pass
- Viability: > 80 %
- Bacteria: Negative
- Mycoplasma: Negative
- Virus (HIV1&2, HBV, HCV, HTLV1&2, Parvovirus B19): Negative
- Karyotype: 46, XX
Sterility: Sterile
Notice To Purchaser: This product is intended for Research Use Only. Commercial Use may require additional agreements. Please contact info-us@reprocell.com for more information. This Product is not intended for Clinical or Diagnostic Use.