iPSC Reprogramming

At REPROCELL, you can gain access to the most rapid integration-free reprogramming method on the market: StemRNA™ 3rd Gen reprogramming technology. StemRNA™ is up to 50 times more efficient than any other non-integrative methodology on the market*, allowing you to access the highest quality iPSCs for your research. Our RNA technology has been used across a diverse range of iPSC projects in the research community, from enhancing in vivo research models^[2] to optimising human iPSC reprogramming^[3].

* Compared with Episomal reprogramming methodology Epi5 (0.03 - 0.04%), StemRNA™ 3rd generation is at least 50 times more efficient (2.00 - 4.00%).

Figure 1. REPROCELL’s StemRNA™ methodology. In just three steps you can access foot-print free, high quality iPSCs using the most efficient reprogramming methodology on the market.

The Benefits of RNA Reprogramming

RNA reprogramming is the optimal method for iPSC establishment^[1,2] and our latest generation technology can be used to transform samples from skin, blood and urine. StemRNA™ uses RNA transgenes, rather than DNA or viral vectors, which is rapidly degraded by cells. The resultant iPSCs therefore possess low transgene persistence, increased chromosomal stability and reduced clonal variation^[4]. As RNA reprogramming leads to decreased copy number variation (CNV), it also reduces the risk of oncogenesis and abnormal karyology^[1].

The Evolution of StemRNA™

At REPROCELL, our dedicated R&D team are always looking for ways to improve our services. For example, since the introduction of our reprogramming technology in 2012, we have increased the number of sources your RNA-iPSCs can be derived from and have extended our human tissue network to allow rapid access to patient samples (Figure 2).

Figure 2. The evolution of StemRNA™ 3^rd Gen Reprograming Technology.

Flexible, Customized Reprogramming

Whether you have your own cells that require reprogramming or wish to source tissues from a select patient group, our expert scientists have the resources and expertise to achieve your research goals. Although typical clinical samples used for REPROCELL reprogramming services are human blood or urine, other options may be possible including alternative species and tissues or cell-lines. You can access our custom iPSC services FAQs here, or find out more on our blog.

Resources

• Scientific Review: The past present and future of RNA reprogramming technology
• White paper: The science behind StemRNA™ 3rd Generation Reprogramming Technology

References

1. Schlaeger et al. A comparison of non-integrating reprogramming methods. Nature Biotechnology 33:1 (2015).
2. Nakajima et al. Establishment of induced pluripotent stem cells from common marmoset fibroblasts by RNA-based reprogramming. Biochemical and Biophysical Research Communications.In Press (2019).
3. Gagliano et al. Microfluidic reprogramming to pluripotency of human somatic cells. Nature Protocols 14 (2019).
4. Paull et al. Automated, high-throughput derivation, characterization and differentiation of induced pluripotent stem cells. Nature Methods 12:9 (2015).
5. Warren et al. Highly Efficient Reprogramming to Pluripotency and Directed Differentiation of Human Cells with Synthetic Modified mRNA. Cell Stem Cell 7:618 (2010).
6. Yakubov et al. Reprogramming of Human Fibroblasts to Pluripotent Stem Cells using mRNA of Four Transcription Factors. Biochemical Biophysical Research Communication. 394:189 (2010).