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Skin Biopsies from Psoriasis Donors

Drug Discovery Assay – reference number: B119

This experiment assesses whether test articles cause a reduction in inflammatory gene expression release in psoriasis skin biopsies, with Betamethasone as a reference compound. It uses clinical skin biopsies obtained from psoriasis donors to explore test article effect on gene expression via rtPCR.

Assay Type: Skin
Tissue: Skin Disease Biopsies
Target: Study Dependant
Control Compound: Betamethasone
Study Type: Ex vivo cultures
Functional Endpoint: Inflammation

Testing Information


The specific results that will be provided are on the anti-inflammatory effect of test articles across psoriasis skin biopsies.

Test Article Requirements

Test article(s) to be provided by the Sponsor in storable aliquots at required test concentrations with information on diluent vehicle used. Stock solutions are prepared in deionized water unless otherwise requested. Sponsor to provide sufficient test article to run the entire study.

Suggested Testing

We suggest test conditions are assessed in singlet across 5–10 donors. 

The set up for a typical full-thickbness human skin tissue explant experiment-1

Diagram showing a typical setup for our skin disease explant assays 

Study Outline

Rationale and Experimental Design

Full-thickness skin biopsies are obtained from our clinical networks. Biopsies are taken at a size of 3mm2 and then transferred into fortified media, leaving the epidermis exposed to air. Biopsies are incubated in optimum conditions for a maximum of 24 hrs, then cultured in the presence of the test article for a further 24 hrs.

An example of the conditions assessed for 1 test article is detailed below (it is recommended a minimum of 5–10 donors should be used for each condition):

  • Control (diseased biopsy)
  • Test article (treated, disease biopsy)
  • Control 2 (non-diseased biopsy)

Exclusion Criteria

No specific exclusion criteria are in place other than to reject macroscopically diseased/necrotic tissue.


Recommended end point analysis includes gene expression, RNA sequencing or immunohistochemistry.

Data from Psoriasis skin biopsies

Psoriasis Patient Biopsies Characterisation by qRT-PCR

Characterization by qRT-PCR: Gene expression profiles are different between non-plaque and plaque biopsies for key psoriasis genes.