StemAb™ Sox2 Antibody (Affinity Purified), Rabbit anti-Mouse/Human

09-0024

Brand: Stemgent™

Purchase product: USA Store

Product Code Pack Size Price in NCSA Price in EMEA
09-0024 100 μL USD $ 275.00 GBP £ 309.67 EUR € 351.49

Prices do not include shipping and handling charges.

stemab-sox2-antibody-affinity-purified-rabbit-anti-mouse-human-p266-183_medium

Sox2, also known as SRY (sex determining region Y)-box 2, is a transcription factor essential for maintaining self-renewal of undifferentiated embryonic stem (ES) cells. Chromatin immunoprecipitation experiments have shown that Sox2 and Oct4 bind to thousands of gene regulatory sites, highlighting the importance of these transcription factors in early embryonic development. The knockdown of Sox2gene expression by siRNA in human ES cells results in loss of the undifferentiated stem cell state. Sox2 is one of the key transcription factors used to reprogram mouse and human fibroblasts to a pluripotent state. The Sox2 Antibody (Affinity Purified) was screened on human and mouse ES cells using immunocytochemistry and flow cytometry and selected as the best antibody available for researchers needing to demonstrate pluripotency.

153684094809-0024_figure1

Figure 1. Immunocytochemistry analysis of Sox2 on R1 mouse ES cells.
Cells were stained with Sox2 Antibody (Affinity Purified) using a 1:100 dilution followed by a secondary Cy3 conjugated antibody (red). DAPI staining was performed to visualize nuclei (blue).

References

  1. Rodda DJ; CHew J-L; Lim L-H; Loh Y-H; Wang B; Ng H-H; Robson P. Transcriptional Regulation of Nanog by OCT4 and SOX2. J Biol Chem 280:24731-7 (2005).
  2. Boyer, L.A., Lee, T.I., Cole, M.F., Johnstone, S.E., Levine, S.S., Zucker, J.P., Guenther, M.G., Kumar, R.M., Murray, H.L., Jenner, R.G., Gifford, D.K., Melton, D.A., Jaenisch, R., and Young, R.A. (2005) Core transcriptional regulatory circuitry in human embryonic stem cells. Cell 122: 947-956.
  3. Fong, H., Hohenstein, K.A., and Donovan, P.J. (2008) Regulation of self-renewal and pluripotency by Sox2 in human embryonic stem cells. Stem Cell 26: 1931-1938.
  4. Takahashi, K., and Yamanaka, S. (2006). Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126, 663-676.
  5. Takahashi, K., Tanabe K., Ohnuki, M., Narita, M., Ichisaka T., Tomoda K. and Yamanaka, S. (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131: 861-872.
  6. Park, I.H., Arora, N., Huo, H., Maherali, N., Ahfeldt, T., Shimamura, A., Lensch, M.W., Cowan, C., Hochedlinger, K., and Daley, G.Q. (2008) Disease-specific induced pluripotent stem cells. Cell 134: 877-886.

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