Skip to megamenu (after main content)
Meet us next:   ELRIG UK 2025 – 20 March  ●  IBD Innovate 2025 – 9-10 April  ●  NIH Spring Vendor Exhibit 2025 – 24 April  ●  AACR Annual Meeting 2025 – 27-30 April  ●  ISCT Conference 2025 – 7-9 May  ●  more on our events calendar

Recombinant human TGF-β3 protein

QK054

Brand: Qkine

Human transforming growth factor beta 3 (TGF-β3) protein is a member of the TGF-β family, a family involved in regulating cell survival, proliferation and differentiation. TGF-β3 is used in human pluripotent stem cell maintenance media.

Qkine TGF-β3 is a highly pure and bioactive 25.4 kDa protein dimer. Qkine growth factors are animal-origin free (AOF) and carrier-protein free (CF) for reproducible results.

Qkine 3-for-2 product campaign

Currency: 

Product name Catalog number Pack size Price Price (USD) Price (GBP) Price (EUR)
Recombinant human TGF-β3 protein, 25 µg QK054-0025 25 µg (select above) $ 355.00 £ 255.00 € 298.00
Recombinant human TGF-β3 protein, 50 µg QK054-0050 50 µg (select above) $ 515.00 £ 380.00 € 444.00
Recombinant human TGF-β3 protein, 100 µg QK054-0100 100 µg (select above) $ 760.00 £ 560.00 € 655.00
Recombinant human TGF-β3 protein, 500 µg QK054-0500 500 µg (select above) $ 3,100.00 £ 2,300.00 € 2,687.00
Recombinant human TGF-β3 protein, 1000 µg QK054-1000 1000 µg (select above) $ 4,950.00 £ 3,600.00 € 4,205.00

Note: prices shown do not include shipping and handling charges.

Qkine company name and logo are the property of Qkine Ltd. UK.

Alternative protein names
Transforming growth factor beta-3, TGF-b3, TGFB3, prepro-transforming growth factor beta-3, arrhythmogenic right ventricular dysplasia 1 (ARVD, ARVD1)
Species reactivity

human

species similarity:
mouse – 100%
rat – 99%
porcine – 98%
bovine – 99%


Summary

  • High purity TGF-β3 (Uniprot: P10600)
  • >98%, by SDS-PAGE quantitative densitometry
  • 12.7 kDa monomer, 25.4 kDa dimer
  • Expressed in E. coli
  • Animal origin-free (AOF) and carrier protein-free
  • Manufactured in Cambridge, UK
  • Lyophilized from acetonitrile, TFA
  • Resuspend in 10 mM HCl at >100 µg/ml (provided with protein and free of charge), prepare single use aliquots, add carrier protein if desired and store frozen at –20°C or –80°C
Handling and Storage FAQ

Featured applications

  • Human pluripotent stem cell maintenance
  • Mesoderm differentiation

Bioactivity

Zebrafish FGF2 / bFGF Qk002 protein bioactivity lot #011

TGF-β3 activity is determined using a TGF-β3 responsive firefly luciferase reporter in HEK293T cells. EC50 = 50 pg/ml. Bioactivity is determined using a TGF-β3 responsive firefly luciferase reporter in HEK293T cells. Cells are treated (in triplicate) with a serial dilution of TGF-β3 for 6 hours. Firefly luciferase activity is measured and normalised to the control Renilla luciferase activity. Data from Qk054 batch #104369.

Purity

Zebrafish FGF2 / bFGF Qk002 protein purity SDS-PAGE lot #011

TGF-β3 migrates as a single band at 25 kDa in non-reducing (NR) conditions and 13 kDa upon reduction (R).  No contaminating protein bands are visible. Purified recombinant protein (3 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250.  Data from Qk054 batch #104369.

Further quality assays

  • Mass spectrometry: single species with expected mass
  • Endotoxin: <0.005 EU/μg protein (below level of detection)
  • Recovery from stock vial:  >95%

Comparison with other suppliers

Qkine TGF-β3 (Qk054) bioactivity was compared with an alternative supplier (Supplier B) using a TGF-β3 responsive firefly luciferase reporter in HEK293T cells (as above). Qkine TGF-β3 and Supplier 1 were found to have equivalent bioactivity, Qk054 EC50 = 50 pg/ml, Supplier 1 EC50 = 67 pg/ml (no significant difference).

Protein background

Transforming growth factor beta 3 (TGF-β3) is a member of the TGF-β family (and TGF-β superfamily). Like other TGF-β family members – TGF-β1 and 2 – TGF-β3 signals via receptor complexes consisting of Type I and Type II serine/threonine kinase receptors, activating intracellular SMAD transcription factors [1]. The TGF-β family have a high degree of overlap/redundancy and regulate a variety of cellular functions, including cell survival, proliferation, and differentiation [2]. TGF-β3 has isoform-specific roles in embryonic palate fusion [3] and wound healing [4].

In culture, TGF-β3 is often used in human pluripotent cell maintenance. TGF-β3 is a component of B8 media – the low-cost weekend-free iPSC media developed in the lab of Paul Burridge, Northwestern University [5]. In the development of B8 media, Kuo et al. proved that while TGF-β1 can maintain iPSCs at 2 ng/ml, TGF-β3 can maintain iPSCs in as little as 0.1 ng/ml, making it a cost-effective alternative to TGF-β1 [5].

Background references

  1. T. Huang, S. L. Schor and A. P. Hinck. Biological Activity Differences between TGF-β1 and TGF-β3 Correlate with Differences in the Rigidity and Arrangement of Their Component Monomers. Biochemistry 2014, 53, 36, 5737–5749. doi.org/10.1021/bi500647d
  2. M. Morikawa, R. Derynck and K. Miyazono. TGF-β and the TGF-β Family: Context-Dependent Roles in Cell and Tissue Physiology. Cold Spring Harb Perspect Biol. 2016 May 2;8(5):a021873. doi: 10.1101/cshperspect.a021873
  3. G, Proetzel, S. A. Pawlowski, M. V. Wiles, M. Yin, G. P. Boivin, P. N. Howles, J. Ding, M. W. Ferguson and T. Doetschman. Transforming growth factor-beta 3 is required for secondary palate fusion. Nat Genet. 1995 Dec;11(4):409-14. doi: 10.1038/ng1295-409
  4. B. Bandyopadhyay, J. Fan, S. Guan, Y. Li, M. Chen, D. T. Woodley, W and Li. A “traffic control” role for TGFbeta3: orchestrating dermal and epidermal cell motility during wound healing. J Cell Biol. 2006 Mar 27;172(7):1093-105. doi: 10.1083/jcb.200507111
  5. H. H. Kuo, X. Gao, J. M. DeKeyser, K. A. Fetterman, E. A. Pinheiro, C. J. Weddle, H. Fonoudi, M. V. Orman, M. Romero-Tejeda, M. Jouni, M. Blancard, T. Magdy, C. L. Epting, A. L. George Jr and P. W. Burridge. Negligible-Cost and Weekend-Free Chemically Defined Human iPSC Culture. Stem Cell Reports. 2020 Feb 11;14(2):256-270. doi: 10.1016/j.stemcr.2019.12.007

Publications using recombinant human TGF-β3 protein (Qk054)

Nutritional requirements of human induced pluripotent stem cells.
In Stem Cell Reports on 13 June 2023 by Lyra-Leite, D. M. et al.
View publication

Thalamocortical organoids enable in vitro modeling of 22q11.2 microdeletion associated with neuropsychiatric disorders.
In Cell Stem Cell on 7 March 2024 by Shin, D., Kim, C.N., Ross, J., Frost, N.A., Sohal, V.S. et al.
View publication

Refined home-brew media for cost-effective, weekend-free hiPSC culture and genetic engineering.
In Open Research Europe 2024 by Truszkowski, L., Bottini, S., Bianchi, S. et al.
View publication