Recombinant human TGF-β3 protein
QK054
Brand: Qkine
Human transforming growth factor beta 3 (TGF-β3) protein is a member of the TGF-β family, a family involved in regulating cell survival, proliferation and differentiation. TGF-β3 is used in human pluripotent stem cell maintenance media.
Qkine TGF-β3 is a highly pure and bioactive 25.4 kDa protein dimer. Qkine growth factors are animal-origin free (AOF) and carrier-protein free (CF) for reproducible results.

Currency:
Product name | Catalog number | Pack size | Price | Price (USD) | Price (GBP) | Price (EUR) |
---|---|---|---|---|---|---|
Recombinant human TGF-β3 protein, 25 µg | QK054-0025 | 25 µg | (select above) | $ 355.00 | £ 255.00 | € 298.00 |
Recombinant human TGF-β3 protein, 50 µg | QK054-0050 | 50 µg | (select above) | $ 515.00 | £ 380.00 | € 444.00 |
Recombinant human TGF-β3 protein, 100 µg | QK054-0100 | 100 µg | (select above) | $ 760.00 | £ 560.00 | € 655.00 |
Recombinant human TGF-β3 protein, 500 µg | QK054-0500 | 500 µg | (select above) | $ 3,100.00 | £ 2,300.00 | € 2,687.00 |
Recombinant human TGF-β3 protein, 1000 µg | QK054-1000 | 1000 µg | (select above) | $ 4,950.00 | £ 3,600.00 | € 4,205.00 |
Note: prices shown do not include shipping and handling charges.
Qkine company name and logo are the property of Qkine Ltd. UK.
Alternative protein names
Species reactivity
human
species similarity:
mouse – 100%
rat – 99%
porcine – 98%
bovine – 99%
Summary
- High purity TGF-β3 (Uniprot: P10600)
- >98%, by SDS-PAGE quantitative densitometry
- 12.7 kDa monomer, 25.4 kDa dimer
- Expressed in E. coli
- Animal origin-free (AOF) and carrier protein-free
- Manufactured in Cambridge, UK
- Lyophilized from acetonitrile, TFA
- Resuspend in 10 mM HCl at >100 µg/ml (provided with protein and free of charge), prepare single use aliquots, add carrier protein if desired and store frozen at –20°C or –80°C
Featured applications
- Human pluripotent stem cell maintenance
- Mesoderm differentiation
Bioactivity

TGF-β3 activity is determined using a TGF-β3 responsive firefly luciferase reporter in HEK293T cells. EC50 = 50 pg/ml. Bioactivity is determined using a TGF-β3 responsive firefly luciferase reporter in HEK293T cells. Cells are treated (in triplicate) with a serial dilution of TGF-β3 for 6 hours. Firefly luciferase activity is measured and normalised to the control Renilla luciferase activity. Data from Qk054 batch #104369.
Purity

TGF-β3 migrates as a single band at 25 kDa in non-reducing (NR) conditions and 13 kDa upon reduction (R). No contaminating protein bands are visible. Purified recombinant protein (3 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250. Data from Qk054 batch #104369.
Further quality assays
- Mass spectrometry: single species with expected mass
- Endotoxin: <0.005 EU/μg protein (below level of detection)
- Recovery from stock vial: >95%
Comparison with other suppliers
Protein background
Transforming growth factor beta 3 (TGF-β3) is a member of the TGF-β family (and TGF-β superfamily). Like other TGF-β family members – TGF-β1 and 2 – TGF-β3 signals via receptor complexes consisting of Type I and Type II serine/threonine kinase receptors, activating intracellular SMAD transcription factors [1]. The TGF-β family have a high degree of overlap/redundancy and regulate a variety of cellular functions, including cell survival, proliferation, and differentiation [2]. TGF-β3 has isoform-specific roles in embryonic palate fusion [3] and wound healing [4].
In culture, TGF-β3 is often used in human pluripotent cell maintenance. TGF-β3 is a component of B8 media – the low-cost weekend-free iPSC media developed in the lab of Paul Burridge, Northwestern University [5]. In the development of B8 media, Kuo et al. proved that while TGF-β1 can maintain iPSCs at 2 ng/ml, TGF-β3 can maintain iPSCs in as little as 0.1 ng/ml, making it a cost-effective alternative to TGF-β1 [5].
Background references
- T. Huang, S. L. Schor and A. P. Hinck. Biological Activity Differences between TGF-β1 and TGF-β3 Correlate with Differences in the Rigidity and Arrangement of Their Component Monomers. Biochemistry 2014, 53, 36, 5737–5749. doi.org/10.1021/bi500647d
- M. Morikawa, R. Derynck and K. Miyazono. TGF-β and the TGF-β Family: Context-Dependent Roles in Cell and Tissue Physiology. Cold Spring Harb Perspect Biol. 2016 May 2;8(5):a021873. doi: 10.1101/cshperspect.a021873
- G, Proetzel, S. A. Pawlowski, M. V. Wiles, M. Yin, G. P. Boivin, P. N. Howles, J. Ding, M. W. Ferguson and T. Doetschman. Transforming growth factor-beta 3 is required for secondary palate fusion. Nat Genet. 1995 Dec;11(4):409-14. doi: 10.1038/ng1295-409
- B. Bandyopadhyay, J. Fan, S. Guan, Y. Li, M. Chen, D. T. Woodley, W and Li. A “traffic control” role for TGFbeta3: orchestrating dermal and epidermal cell motility during wound healing. J Cell Biol. 2006 Mar 27;172(7):1093-105. doi: 10.1083/jcb.200507111
- H. H. Kuo, X. Gao, J. M. DeKeyser, K. A. Fetterman, E. A. Pinheiro, C. J. Weddle, H. Fonoudi, M. V. Orman, M. Romero-Tejeda, M. Jouni, M. Blancard, T. Magdy, C. L. Epting, A. L. George Jr and P. W. Burridge. Negligible-Cost and Weekend-Free Chemically Defined Human iPSC Culture. Stem Cell Reports. 2020 Feb 11;14(2):256-270. doi: 10.1016/j.stemcr.2019.12.007
Publications using recombinant human TGF-β3 protein (Qk054)
Nutritional requirements of human induced pluripotent stem cells.
In Stem Cell Reports on 13 June 2023 by Lyra-Leite, D. M. et al.
View publication
Thalamocortical organoids enable in vitro modeling of 22q11.2 microdeletion associated with neuropsychiatric disorders.
In Cell Stem Cell on 7 March 2024 by Shin, D., Kim, C.N., Ross, J., Frost, N.A., Sohal, V.S. et al.
View publication
Refined home-brew media for cost-effective, weekend-free hiPSC culture and genetic engineering.
In Open Research Europe 2024 by Truszkowski, L., Bottini, S., Bianchi, S. et al.
View publication