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Recombinant human noggin protein

QK034

Brand: Qkine

Human noggin is a bone morphogenetic protein (BMP) antagonist which regulates cell differentiation and growth. Noggin is used in the culture of intestinal, pancreatic, lung and tumor-derived organoids and the maintenance of undifferentiated embryonic stem cells (ESC) and for stem cell differentiation into neural and microglial lineages.

Qkine human noggin (Qk034) is a highly pure, animal origin-free, bioactive 46 kDa dimer for reproducible results in organoid culture.

Qkine 3-for-2 product campaign

Currency: 

Product name Catalog number Pack size Price Price (USD) Price (GBP) Price (EUR)
Recombinant human noggin protein, 25 µg QK034-0025 25 µg (select above) $ 280.00 £ 205.00 € 240.00
Recombinant human noggin protein, 50 µg QK034-0050 50 µg (select above) $ 410.00 £ 305.00 € 357.00
Recombinant human noggin protein, 100 µg QK034-0100 100 µg (select above) $ 620.00 £ 455.00 € 532.00
Recombinant human noggin protein, 500 µg QK034-0500 500 µg (select above) $ 2,500.00 £ 1,840.00 € 2,150.00
Recombinant human noggin protein, 1000 µg QK034-1000 1000 µg (select above) $ 3,950.00 £ 2,900.00 € 3,388.00

Note: prices shown do not include shipping and handling charges.

Qkine company name and logo are the property of Qkine Ltd. UK.

Alternative protein names
Synostoses (multiple) syndrome 1, Symphalangism 1 (proximal)
Species reactivity

Human

Species similarity:
mouse – 99%
rat – 99%
bovine – 99%
porcine – 99%

Frequently used together with:

Summary

  • High purity human noggin protein (Uniprot: Q13253)
  • >98%, by SDS-PAGE quantitative densitometry
  • 46 kDa (dimer)
  • Expressed in E. coli
  • Animal origin-free (AOF) and carrier protein-free.
  • Manufactured in Qkine's Cambridge, UK laboratories
  • Lyophilized from acetonitrile, TFA
  • Resuspend in 10 mM HCl at >100 µg/ml (provided with protein and free of charge), prepare single use aliquots, add carrier protein if desired and store frozen at -20°C or -80°C
Handling and Storage FAQ

Featured applications

  • Tumor organoid culture
  • Epithelial organoid culture (WNR media)
  • Stem cell differentiation into neural and microglial lineages

Bioactivity

Human Noggin Qk034 protein bioactivity lot #104285

Human noggin is a BMP inhibitor, and its activity is determined by inhibition of BMP-2 (Qk007) activity in a BMP-2 responsive firefly luciferase reporter assay. HEK293T cells are treated in triplicate with a serial dilution of noggin and a standard concentration of BMP-2 for 6 hours. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity. EC50 = 1.5 nM (70.1 ng/ml). Data from Qk034 lot #104291.

 

Purity

Human Noggin Qk034 protein purity SDS-PAGE lot 104285

Noggin protein (Qk034) has an unusual migration in non-reduced (NR) SDS-PAGE due to the non-covalent dimer which is the active protein. Similar migration in SDS-PAGE is seen for gremlin-1, a related BMP antagonist. The identity of the purified dimeric protein was confirmed using mass spectrometry. Upon reduction, the protein monomer migrates at 23 kDa. Purified recombinant human noggin protein (7 μg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptoethanol, R) and non-reduced conditions (NR) and stained with Coomassie Brilliant Blue R250. Data from Qk034 lot #104285.

Further quality assays

  • Mass spectrometry: single species with expected mass
  • Analytical reversed-phase: single sharp peak
  • Endotoxin: <0.005 EU/μg protein (below level of detection)
  • Recovery from stock vial:  >95%

Qkine human noggin is as biologically active as a comparable alternative supplier protein

Quantitative luciferase assay with Qkine human noggin (Qk034, green) and alternative supplier noggin (Supplier B, black) inhibition of BMP-2 (Qk007). Cells were treated in triplicate with a serial dilution of noggin and a standard concentration of BMP-2 for 6 hours. Firefly luciferase activity was measured and normalized to control Renilla luciferase activity.

Protein background

Noggin is a 64 kDa homodimeric glycoprotein expressed during embryonic development and in adult cells [1]. Noggin is an extracellular antagonist of BMP cell signaling. BMPs are part of the larger TGFβ family and regulate embryonic, fetal and adult development in all vertebrates [1, 2]. Noggin binds to BMP-2, -4, -6 and -7, preventing interaction of the ligands with their receptor, and consequently blocking downstream activation of signaling cascades [2,3].

Through its control of BMP signaling, noggin plays a crucial role in embryonic patterning, as well as in the development of the neural tube, teeth, hair follicles, and the eye [4]. In cell cultures, noggin is used in the maintenance of pluripotent stem cells, differentiation of iPSCs to neural lineages, and in a large variety of organoid culture systems including intestine, liver and lung organoids [4, 5].

Background references

  1. Krause, C., Guzman, A. and Knaus, P. Noggin, Int. J. Biochem. Cell Biol., 43:4 (2011). doi.org/10.1016/j.biocel.2011.01.007.
  2. La Rosa, I., Camargo, L.S., Pereira, M.M. et al. Effects of bone morphogenic protein 4 (BMP4) and its inhibitor, Noggin, on in vitro maturation and culture of bovine preimplantation embryos. Reprod Biol Endocrinol 9:18 (2011). doi.org/10.1186/1477-7827-9-18.
  3. Rifas, L. The Role of Noggin in Human Mesenchymal Stem Cell Differentiation. J. Cell. Biochem. 100:824–834 (2007). doi.org/10.1002/jcb.21132.
  4. Urbischek, M., Rannikmae, H., Foets, T. et al. Organoid culture media formulated with growth factors of defined cellular activity. Sci Rep 9, 6193 (2019). doi.org/10.1038/s41598-019-42604-0
  5. Wang,G., Zhang, H., Zhao, Y., Li, J., Cai, J., Wang, P., Meng, S., Feng, J., Miao, C., Ding, M., Li, D. and Deng, H. Noggin and bFGF cooperate to maintain the pluripotency of human embryonic stem cells in the absence of feeder layers. Biochem Biophys Res Commun 330:3 (2005). doi.org/10.1016/j.bbrc.2005.03.058.

Publications using recombinant human noggin protein (Qk034)

Human epidermis organotypic cultures, a reproducible system recapitulating the epidermis in vitro
In Experimental Dermatology on 28 April 2023 by Agarwal, R. et al.
 View publication 

Spatial profiling of early primate gastrulation in utero
In Nature on 16 June 2022 by Bergmann, S., Penfold, C.A., Slatery, E. et al.
 View publication 

The effect of extracellular matrix on the precision medicine utility of pancreatic cancer patient–derived organoids
In JCI Insight on 5 December 2023 by Lumibao, J. C., Okhovat, S. et al.
 View publication