NutriStem™ hIPSC XF Culture Medium for Human iPS and ES Cells

01-0005 / 01-0005-100

Brand: NutriStem™ by Biological Industries®

Purchase product: USA Store

Product Code Pack Size Price in
The Americas
Price in EMEA
01-0005 500 mL USD $ 215.00 GBP £ 215.00 EUR € 244.03
01-0005-100 100 mL USD $ 65.00 GBP £ 55.00 EUR € 62.43

Prices do not include shipping and handling charges

nutristem-hpsc-xf-medium-p199-74_image

Notice to clinical users: A Drug Master File for NutriStem medium has been filed with the US FDA. Please contact us for more information.

NutriStem hPSC XF Medium (formerly called NutriStem XF/FF Culture Medium) is a fully defined xeno-free, low growth factor human embryonic stem (ES) and induced pluripotent stem (iPS) cell feeder-free culture medium that enables maintenance and expansion of pluripotent stem cells. NutriStem Medium can be used to culture pluripotent stem cells for at least 20 passages while retaining pluripotency marker expression, robust proliferation with a normal karyotype, and the ability to differentiate into cells of all three germ layers in vitro and in vivo. NutriStem Medium offers the ability to culture cells in a completely xeno-free medium without the need for high levels of basic FGF and other stimulatory growth factors and cytokines. In addition, the superior cell attachment and proliferation observed with NutriStem Medium aid high-throughput screening applications.

  • Xeno-free, feeder-free conditions contain no animal components
  • Easy, one-step transition from feeder-dependent culture, no adaptation required
  • Maintains pluripotency, normal morphology, karyotype, and differentiation potential of human ES and iPS cells over long term culture
  • Robust attachment and high cloning efficiency from single cells

 

1536921201NutriStem_Fig1
Morphology of Human H1 ES cells Grown in NutriStem hPSC XF Medium for 20 passages.
1536921184NutriStem_Fig2
Expression of Pluripotency Markers in hES Cells Cultured in NutriStem hPSC XF medium.
1536842370010005_figure3Proliferation Growth Rate of Human H1 ES cells Cultured in NutriStem hPSC XF Medium versus Competitor Medium.

Specifications

Product Name: NutriStem hPSC XF Medium

Catalog Number

  • 01-0005 (Biological Industries Cat # 05-100-1A): 500 mL
  • 01-0005-100 (Biological Industries Cat # 05-100-1B): 100 mL

Storage and Stability: Store at −20°C. This product is stable for a minimum of 3 months when stored as directed.

Quality Control: NutriStem Medium tested negative for mycoplasma. Human pluripotent stem cells expressed the pluripotency markers SSEA-4, TRA-1-60, and TRA-1-81 after three continuous passages in NutriStem Medium

Sterility: Sterile

Notice To Purchaser: REPROCELL is an authorized global distributor of select cell culture products from Biological Industries, Inc. (Israel).

Manufacturer: Biological Industries, Inc. (Israel)

Related Products:

  • StemRNA 3rd Gen Reprogramming Kit
  • StemRNA-SR Reprogramming Kit
  • CryoStem Freezing Medium

Documents

Product Flyers

Protocols

Application Notes

References